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Pangenome04-0298108BA0217611819-9771193COLECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50N315NCTC8325NewmanRF122ST398T0131TCH60TW20USA300_FPR3757USA300_TCH1516VC40
NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00779
  • pan locus tag?: SAUPAN002675000
  • symbol: SAOUHSC_00779
  • pan gene symbol?: uvrB2
  • synonym:
  • product: excinuclease ABC subunit B

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_00779
  • symbol: SAOUHSC_00779
  • product: excinuclease ABC subunit B
  • replicon: chromosome
  • strand: +
  • coordinates: 758680..759654
  • length: 975
  • essential: no DEG

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGATTGATGAATCACATGTGACATTACCGCAAGTTCGAGGCATGTATAACGGAGACAGA
    GCGCGTAAACAAGTTTTGGTGGATCATGGGTTTAGATTACCGAGTGCATTAGATAACCGT
    CCACTTAAATTTGAAGAATTTGAAGAAAAGACAAAACAACTTGTGTATGTATCTGCAACG
    CCTGGACCATACGAAATTGAACATACGGATAAGATGGTTGAACAAATTATTCGTCCTACT
    GGTTTACTGGATCCTAAGATTGAGGTTAGACCTACTGAAAATCAAATTGACGATTTATTA
    AGTGAAATTCAAACAAGAGTTGAGCGTAATGAACGCGTACTTGTTACAACGCTCACTAAA
    AAGATGAGTGAAGATTTAACCACATACATGAAAGAAGCGGGTATTAAAGTTAATTATCTG
    CATTCAGAAATCAAGACATTAGAACGAATTGAAATAATTAGAGACTTACGAATGGGTACA
    TATGATGTTATCGTAGGTATTAATTTATTAAGAGAGGGTATTGATATACCAGAAGTTTCT
    CTAGTTGTCATATTAGATGCAGATAAAGAAGGGTTTTTACGTTCTAACCGCTCATTAATT
    CAAACAATAGGTAGAGCTGCGCGTAACGATAAAGGTGAAGTCATTATGTATGCCGATAAA
    ATGACTGATTCGATGAAGTATGCAATTGATGAGACACAACGTCGTCGAGAAATACAGATG
    AAACATAATGAAAAACATGGTATTACACCTAAAACAATTAATAAAAAAATACATGATTTA
    ATTAGTGCTACTGTTGAAAATGACGAAAATAATGACAAAGCACAAACTGTGATACCTAAG
    AAGATGACGAAAAAAGAACGTCAAAAGACAATCGACAATATAGAAAAAGAAATGAAACAA
    GCAGCGAAAGATTTAGATTTCGAGAAAGCTACAGAATTAAGAGATATGTTATTTGAATTA
    AAAGCAGAAGGGTGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    975

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_00779
  • symbol: SAOUHSC_00779
  • description: excinuclease ABC subunit B
  • length: 324
  • theoretical pI: 6.21157
  • theoretical MW: 37476.8
  • GRAVY: -0.672531

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Genetic information processingDNA metabolismDNA replication, recombination, and repairexcinuclease ABC subunit B (TIGR00631; EC 3.1.25.-; HMM-score: 533.6)
    Genetic information processingDNA metabolismDNA replication, recombination, and repairATP-dependent DNA helicase RecG (TIGR00643; EC 3.6.4.12; HMM-score: 33.7)
    Genetic information processingDNA metabolismDNA replication, recombination, and repairprimosomal protein N' (TIGR00595; EC 3.6.1.-; HMM-score: 31.1)
    DNA phosphorothioation system restriction enzyme (TIGR04095; HMM-score: 31)
    Genetic information processingDNA metabolismDNA replication, recombination, and repairATP-dependent DNA helicase, RecQ family (TIGR00614; EC 3.6.4.12; HMM-score: 30.2)
    Genetic information processingDNA metabolismDNA replication, recombination, and repairATP-dependent DNA helicase RecQ (TIGR01389; EC 3.6.4.12; HMM-score: 29.9)
    Genetic information processingDNA metabolismDNA replication, recombination, and repairtranscription-repair coupling factor (TIGR00580; EC 3.6.1.-; HMM-score: 20.8)
    DEXH box helicase, DNA ligase-associated (TIGR04121; EC 3.6.4.-; HMM-score: 18.3)
    Genetic information processingDNA metabolismDNA replication, recombination, and repairexcinuclease ABC subunit C (TIGR00194; EC 3.1.25.-; HMM-score: 17)
    helicase/secretion neighborhood putative DEAH-box helicase (TIGR03817; HMM-score: 15.3)
    CRISPR type I-A/APERN-associated protein Csa5 (TIGR01878; HMM-score: 5.7)
  • TheSEED:  
    DNA MetabolismDNA repairDNA repair, UvrABC system Excinuclease ABC subunit B 
  • PFAM:
    P-loop_NTPase (CL0023) UvrB; Ultra-violet resistance protein B (PF12344; HMM-score: 85.8)
    Helicase_C; Helicase conserved C-terminal domain (PF00271; HMM-score: 75.6)
    no clan definedUVR; UvrB/uvrC motif (PF02151; HMM-score: 36.6)
    PDDEXK (CL0236) Sen15; Sen15 protein (PF09631; HMM-score: 13.9)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.115
    • Ymax_pos: 31
    • Cmax: 0.109
    • Cmax_pos: 21
    • Smax: 0.22
    • Smax_pos: 22
    • Smean: 0.099
    • D: 0.109
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MIDESHVTLPQVRGMYNGDRARKQVLVDHGFRLPSALDNRPLKFEEFEEKTKQLVYVSATPGPYEIEHTDKMVEQIIRPTGLLDPKIEVRPTENQIDDLLSEIQTRVERNERVLVTTLTKKMSEDLTTYMKEAGIKVNYLHSEIKTLERIEIIRDLRMGTYDVIVGINLLREGIDIPEVSLVVILDADKEGFLRSNRSLIQTIGRAARNDKGEVIMYADKMTDSMKYAIDETQRRREIQMKHNEKHGITPKTINKKIHDLISATVENDENNDKAQTVIPKKMTKKERQKTIDNIEKEMKQAAKDLDFEKATELRDMLFELKAEG

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator: SAOUHSC_01333 (LexA*)
    LexA* (TF) important in SOS response

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-3661
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]