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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00776
  • pan locus tag?: SAUPAN002672000
  • symbol: SAOUHSC_00776
  • pan gene symbol?: uvrB
  • synonym:
  • product: excinuclease ABC subunit B

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_00776
  • symbol: SAOUHSC_00776
  • product: excinuclease ABC subunit B
  • replicon: chromosome
  • strand: +
  • coordinates: 756470..757501
  • length: 1032
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGGTTGAACATTATCCTTTTAAAATACATTCTGATTTTGAGCCTCAAGGTGACCAACCG
    CAAGCAATTAAAGAAATCGTGGAAGGTATTAAAGCGGGGAAAAGACATCAAACTTTATTA
    GGTGCTACTGGCACAGGGAAAACATTTACGATGAGTAATGTTATTAAAGAAGTTGGGAAA
    CCAACGTTAATTATCGCGCATAACAAAACATTAGCAGGACAATTATATAGTGAGTTTAAA
    GAATTTTTTCCTGAAAACAGGGTGGAATACTTTGTAAGTTACTATGATTATTATCAACCA
    GAGGCATACGTACCGTCTACTGACACTTTTATTGAAAAAGATGCCTCAATCAATGATGAA
    ATTGATCAACTACGACATTCTGCTACAAGTGCATTATTTGAACGCGATGATGTAATTATT
    ATTGCTAGTGTAAGTTGTATATATGGTTTAGGTAATCCTGAAGAATATAAAGATTTAGTA
    GTAAGTGTTCGAGTTGGTATGGAAATGGATAGAAGTGAATTACTTAGAAAACTTGTAGAT
    GTGCAATATACACGAAATGACATCGATTTCCAACGAGGAACGTTTCGAGTGCGTGGTGAT
    GTAGTGGAAATATTCCCAGCCTCTAAAGAAGAACTTTGTATAAGGGTTGAGTTTTTCGGC
    GATGAGATTGACCGTATCCGAGAAGTTAACTACCTAACAGGTGAAGTGTTGAAAGAAAGA
    GAACATTTTGCGATATTCCCAGCTTCTCACTTCGTAACACGTGAAGAAAAGTTGAAAGTT
    GCGATTGAACGTATTGAAAAAGAATTGGAAGAACGATTGAAAGAATTACGAGATGAGAAT
    AAATTACTAGAAGCGCAAAGGTTAGAACAGCGTACCAACTATGATTTAGAAATGATGCGA
    GAGATGGGATTCTGTTCAGGAATTGAAAACTATTCCGTACATTTAACTTTGCGACCACTG
    GGTTCGACACCATATACTTTATTGGATTACTTTGGCGATGATTGGTTAGGGAAGGTGCGA
    ACAAGTCCCTGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1032

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_00776
  • symbol: SAOUHSC_00776
  • description: excinuclease ABC subunit B
  • length: 343
  • theoretical pI: 4.74824
  • theoretical MW: 39815.8
  • GRAVY: -0.468222

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Genetic information processingDNA metabolismDNA replication, recombination, and repairexcinuclease ABC subunit B (TIGR00631; EC 3.1.25.-; HMM-score: 556.6)
    Genetic information processingDNA metabolismDNA replication, recombination, and repairtranscription-repair coupling factor (TIGR00580; EC 3.6.1.-; HMM-score: 56.3)
    Genetic information processingDNA metabolismDNA replication, recombination, and repairATP-dependent DNA helicase RecG (TIGR00643; EC 3.6.4.12; HMM-score: 18.5)
    DNA phosphorothioation system restriction enzyme (TIGR04095; HMM-score: 11.6)
    Cellular processesCellular processesChemotaxis and motilityflagellar biosynthesis protein FlhF (TIGR03499; HMM-score: 8.5)
  • TheSEED:  
    DNA MetabolismDNA repairDNA repair, UvrABC system Excinuclease ABC subunit B 
  • PFAM:
    P-loop_NTPase (CL0023) ResIII; Type III restriction enzyme, res subunit (PF04851; HMM-score: 49.9)
    DEAD; DEAD/DEAH box helicase (PF00270; HMM-score: 20.6)
    AAA_30; AAA domain (PF13604; HMM-score: 19.5)
    AAA; ATPase family associated with various cellular activities (AAA) (PF00004; HMM-score: 15.9)
    Kinesin; Kinesin motor domain (PF00225; HMM-score: 15.5)
    TrwB_AAD_bind; Type IV secretion-system coupling protein DNA-binding domain (PF10412; HMM-score: 14.5)
    Microtub_bd; Microtubule binding (PF16796; HMM-score: 14.3)
    DUF853; Bacterial protein of unknown function (DUF853) (PF05872; HMM-score: 14.2)
    DUF87; Domain of unknown function DUF87 (PF01935; HMM-score: 13.6)
    AAA_25; AAA domain (PF13481; HMM-score: 12.5)
    AAA_22; AAA domain (PF13401; HMM-score: 11.9)
    no clan definedRsa3; Ribosome-assembly protein 3 (PF14615; HMM-score: 8.6)

Structure, modifications & interactions[edit source | edit]

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.108
    • Ymax_pos: 67
    • Cmax: 0.117
    • Cmax_pos: 58
    • Smax: 0.126
    • Smax_pos: 22
    • Smean: 0.086
    • D: 0.099
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MVEHYPFKIHSDFEPQGDQPQAIKEIVEGIKAGKRHQTLLGATGTGKTFTMSNVIKEVGKPTLIIAHNKTLAGQLYSEFKEFFPENRVEYFVSYYDYYQPEAYVPSTDTFIEKDASINDEIDQLRHSATSALFERDDVIIIASVSCIYGLGNPEEYKDLVVSVRVGMEMDRSELLRKLVDVQYTRNDIDFQRGTFRVRGDVVEIFPASKEELCIRVEFFGDEIDRIREVNYLTGEVLKEREHFAIFPASHFVTREEKLKVAIERIEKELEERLKELRDENKLLEAQRLEQRTNYDLEMMREMGFCSGIENYSVHLTLRPLGSTPYTLLDYFGDDWLGKVRTSP

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 1.16 1.17 1.18 1.19 1.20 1.21 1.22 1.23 1.24 1.25 1.26 1.27 1.28 1.29 1.30 1.31 1.32 1.33 1.34 1.35 1.36 1.37 1.38 1.39 1.40 1.41 1.42 1.43 1.44 1.45 1.46 1.47 1.48 1.49 1.50 1.51 1.52 1.53 1.54 1.55 1.56 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)
  2. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-3661
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]