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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01287
  • pan locus tag?: SAUPAN003626000
  • symbol: SAOUHSC_01287
  • pan gene symbol?: glnA
  • synonym:
  • product: glutamine synthetase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_01287
  • symbol: SAOUHSC_01287
  • product: glutamine synthetase
  • replicon: chromosome
  • strand: +
  • coordinates: 1242808..1244148
  • length: 1341
  • essential: yes [1] DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGCCAAAACGTACTTTCACTAAAGACGACATTCGTAAATTTGCAGAAGAGGAAAATGTA
    AGATATTTAAGATTACAATTCACTGATATTTTAGGAACAATTAAAAATGTTGAAGTGCCT
    GTAAGCCAATTAGAAAAAGTACTTGATAACGAAATGATGTTTGACGGTTCTTCTATCGAA
    GGTTTCGTACGTATCGAAGAATCAGATATGTACTTACATCCAGATTTAGATACTTGGGTA
    ATCTTCCCATGGACTGCTGGACAAGGTAAAGTTGCACGTTTAATTTGTGATGTATATAAA
    ACAGATGGAACACCATTTGAAGGGGATCCTCGTGCAAACTTAAAACGTGTATTAAAAGAA
    ATGGAAGATTTAGGCTTCACAGACTTTAACCTAGGACCTGAACCAGAATTCTTCTTGTTT
    AAGTTGGATGAAAAAGGGGAACCAACTTTAGAACTTAATGATGATGGTGGATATTTCGAT
    TTAGCACCTACAGATTTAGGTGAAAACTGTCGTCGTGATATTGTTTTAGAATTAGAGGAT
    ATGGGCTTCGATATTGAAGCTAGTCACCATGAAGTTGCCCCTGGTCAACATGAAATTGAC
    TTTAAATATGCAGATGCTGTTACAGCATGTGATAATATCCAAACATTTAAATTGGTTGTT
    AAAACAATCGCACGTAAACATAATTTACACGCAACATTTATGCCTAAACCATTATTCGGT
    GTGAATGGTAGCGGTATGCACTTTAACGTTTCATTATTCAAAGGTAAAGAAAATGCATTC
    TTTGATCCAAATACTGAAATGGGCTTAACGGAAACTGCATATCAATTTACAGCAGGTGTG
    CTTAAAAATGCACGCGGATTTACTGCTGTATGTAACCCGTTAGTAAACTCATATAAACGT
    TTAGTACCTGGTTATGAAGCACCATGTTATATTGCATGGAGTGGTAAAAACCGTTCACCA
    TTAATCCGTGTACCATCTTCAAGAGGATTATCTACTCGTATCGAAGTACGTTCAGTAGAT
    CCAGCTGCAAACCCATACATGGCGTTAGCTGCAATCTTAGAAGCTGGACTAGATGGTATT
    AAAAATAAATTAAAAGTTCCAGAACCAGTTAACCAAAATATTTACGAAATGAACCGTGAA
    GAACGTGAAGCAGTAGGCATTCAAGACTTACCTTCAACACTTTATACTGCATTAAAAGCA
    ATGCGTGAAAATGAAGTTATTAAAAAAGCTTTAGGAAATCATATCTATAATCAATTTATT
    AATTCAAAATCAATTGAATGGGATTACTACAGAACTCAAGTCTCTGAATGGGAAAGAGAT
    CAGTACATGAAGCAATATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1341

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_01287
  • symbol: SAOUHSC_01287
  • description: glutamine synthetase
  • length: 446
  • theoretical pI: 4.83865
  • theoretical MW: 50840.4
  • GRAVY: -0.438117

Function[edit source | edit]

  • reaction:
    EC 6.3.1.2?  ExPASy
    Glutamate--ammonia ligaseATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine
  • TIGRFAM:
    MetabolismAmino acid biosynthesisGlutamate familyglutamine synthetase, type I (TIGR00653; EC 6.3.1.2; HMM-score: 581.8)
    glutamine synthetase, type III (TIGR03105; EC 6.3.1.2; HMM-score: 367.9)
  • TheSEED:  
    Amino Acids and DerivativesGlutamine, glutamate, aspartate, asparagine; ammonia assimilationGlutamine synthetases Glutamine synthetase type I (EC 6.3.1.2) 
    Glutamine, glutamate, aspartate, asparagine; ammonia assimilationGlutamine, Glutamate, Aspartate and Asparagine Biosynthesis Glutamine synthetase type I (EC 6.3.1.2) 
    Cell Wall and CapsuleCell Wall and Capsule - no subcategoryPeptidoglycan Biosynthesis Glutamine synthetase type I (EC 6.3.1.2) 
    Nitrogen MetabolismNitrogen Metabolism - no subcategoryAmmonia assimilation Glutamine synthetase type I (EC 6.3.1.2) 
  • PFAM:
    GCS (CL0286) Gln-synt_C; Glutamine synthetase, catalytic domain (PF00120; HMM-score: 453.9)
    no clan definedGln-synt_N; Glutamine synthetase, beta-Grasp domain (PF03951; HMM-score: 110.4)
    Gln-synt_N_2; Glutamine synthetase N-terminal domain (PF16952; HMM-score: 12.3)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 10
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.107
    • Ymax_pos: 44
    • Cmax: 0.116
    • Cmax_pos: 44
    • Smax: 0.128
    • Smax_pos: 38
    • Smean: 0.083
    • D: 0.098
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MPKRTFTKDDIRKFAEEENVRYLRLQFTDILGTIKNVEVPVSQLEKVLDNEMMFDGSSIEGFVRIEESDMYLHPDLDTWVIFPWTAGQGKVARLICDVYKTDGTPFEGDPRANLKRVLKEMEDLGFTDFNLGPEPEFFLFKLDEKGEPTLELNDDGGYFDLAPTDLGENCRRDIVLELEDMGFDIEASHHEVAPGQHEIDFKYADAVTACDNIQTFKLVVKTIARKHNLHATFMPKPLFGVNGSGMHFNVSLFKGKENAFFDPNTEMGLTETAYQFTAGVLKNARGFTAVCNPLVNSYKRLVPGYEAPCYIAWSGKNRSPLIRVPSSRGLSTRIEVRSVDPAANPYMALAAILEAGLDGIKNKLKVPEPVNQNIYEMNREEREAVGIQDLPSTLYTALKAMRENEVIKKALGNHIYNQFINSKSIEWDYYRTQVSEWERDQYMKQY

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Roy R Chaudhuri, Andrew G Allen, Paul J Owen, Gil Shalom, Karl Stone, Marcus Harrison, Timothy A Burgis, Michael Lockyer, Jorge Garcia-Lara, Simon J Foster, Stephen J Pleasance, Sarah E Peters, Duncan J Maskell, Ian G Charles
    Comprehensive identification of essential Staphylococcus aureus genes using Transposon-Mediated Differential Hybridisation (TMDH).
    BMC Genomics: 2009, 10;291
    [PubMed:19570206] [WorldCat.org] [DOI] (I e)
  2. Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-3661
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  4. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]