From AureoWiki
Jump to: navigation, search
NCBI: 26-AUG-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1150 [new locus tag: SA_RS06490 ]
  • pan locus tag?: SAUPAN003626000
  • symbol: glnA
  • pan gene symbol?: glnA
  • synonym:
  • product: glutamine-ammonia ligase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA1150 [new locus tag: SA_RS06490 ]
  • symbol: glnA
  • product: glutamine-ammonia ligase
  • replicon: chromosome
  • strand: +
  • coordinates: 1307165..1308505
  • length: 1341
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGCCAAAACGTACTTTCACTAAAGAAGACATTCGTAAATTTGCAGAAGAGGAAAATGTA
    AGATATTTAAGATTACAATTCACTGATATTTTAGGAACAATTAAAAATGTTGAAGTGCCT
    GTAAGCCAATTAGAAAAAGTACTTGATAACGAAATGATGTTTGACGGTTCTTCTATCGAA
    GGTTTCGTACGTATCGAAGAATCAGATATGTACTTACATCCAGATTTAGATACTTGGGTA
    ATCTTCCCATGGACTGCTGGACAAGGTAAAGTTGCACGTTTAATTTGTGATGTATATAAA
    ACAGATGGAACACCATTTGAAGGGGATCCTCGTGCAAACTTAAAACGTGTATTAAAAGAA
    ATGGAAGATTTAGGCTTCACAGACTTTAACCTAGGGCCTGAACCAGAATTCTTCTTGTTT
    AAGTTGGATGAAAAAGGGGAACCAACTTTAGAACTTAATGATGATGGTGGATATTTCGAT
    TTAGCACCTACAGATTTAGGTGAAAACTGTCGTCGTGATATTGTTTTAGAATTAGAGGAT
    ATGGGCTTCGATATTGAAGCTAGTCACCATGAAGTTGCCCCTGGTCAACATGAAATTGAC
    TTTAAATATGCAGATGCTGTTACAGCATGTGATAATATCCAAACATTTAAATTGGTTGTT
    AAAACAATCGCACGTAAACATAATTTACACGCAACATTTATGCCTAAACCATTATTCGGT
    GTGAATGGTAGCGGTATGCACTTTAACGTTTCATTATTCAAAGGTAAAGAAAATGCATTC
    TTTGATCCAAATACTGAAATGGGCTTAACGGAAACTGCATATCAATTTACAGCAGGTGTG
    CTTAAAAATGCACGCGGATTTACTGCTGTATGTAACCCGTTAGTAAACTCATATAAACGT
    TTAGTACCTGGTTATGAAGCACCATGTTATATTGCATGGAGTGGTAAAAACCGTTCACCA
    TTAATCCGTGTACCATCTTCAAGAGGATTATCTACTCGTATCGAAGTACGTTCAGTAGAT
    CCAGCTGCAAACCCATACATGGCGTTAGCTGCAATCTTAGAAGCTGGACTAGATGGTATT
    AAAAATAAATTAAAAGTTCCAGAACCAGTTAACCAAAATATTTACGAAATGAACCGTGAA
    GAACGTGAAGCAGTAGGCATTCAAGACTTACCTTCAACACTTTATACTGCATTAAAAGCA
    ATGCGTGAAAATGAAGTTATTAAAAAAGCTTTAGGAAATCATATCTATAATCAATTTATT
    AATTCAAAATCAATTGAATGGGATTACTACAGAACTCAAGTATCTGAATGGGAAAGAGAT
    CAGTACATGAAGCAATATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1341

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA1150 [new locus tag: SA_RS06490 ]
  • symbol: GlnA
  • description: glutamine-ammonia ligase
  • length: 446
  • theoretical pI: 4.84142
  • theoretical MW: 50854.4
  • GRAVY: -0.438117

Function[edit source | edit]

  • reaction:
    EC 6.3.1.2?  ExPASy
    Glutamate--ammonia ligaseATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine
  • TIGRFAM:
    MetabolismAmino acid biosynthesisGlutamate familyglutamine synthetase, type I (TIGR00653; EC 6.3.1.2; HMM-score: 582.1)
    glutamine synthetase, type III (TIGR03105; EC 6.3.1.2; HMM-score: 367.9)
  • TheSEED:  
    Amino Acids and DerivativesGlutamine, glutamate, aspartate, asparagine; ammonia assimilationGlutamine synthetases Glutamine synthetase type I (EC 6.3.1.2) 
    Glutamine, glutamate, aspartate, asparagine; ammonia assimilationGlutamine, Glutamate, Aspartate and Asparagine Biosynthesis Glutamine synthetase type I (EC 6.3.1.2) 
    Cell Wall and CapsuleCell Wall and Capsule - no subcategoryPeptidoglycan Biosynthesis Glutamine synthetase type I (EC 6.3.1.2) 
    Nitrogen MetabolismNitrogen Metabolism - no subcategoryAmmonia assimilation Glutamine synthetase type I (EC 6.3.1.2) 
  • PFAM:
    GCS (CL0286) Gln-synt_C; Glutamine synthetase, catalytic domain (PF00120; HMM-score: 453.9)
    no clan definedGln-synt_N; Glutamine synthetase, beta-Grasp domain (PF03951; HMM-score: 110.4)
    Gln-synt_N_2; Glutamine synthetase N-terminal domain (PF16952; HMM-score: 12.5)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.107
    • Ymax_pos: 44
    • Cmax: 0.116
    • Cmax_pos: 44
    • Smax: 0.135
    • Smax_pos: 1
    • Smean: 0.086
    • D: 0.099
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MPKRTFTKEDIRKFAEEENVRYLRLQFTDILGTIKNVEVPVSQLEKVLDNEMMFDGSSIEGFVRIEESDMYLHPDLDTWVIFPWTAGQGKVARLICDVYKTDGTPFEGDPRANLKRVLKEMEDLGFTDFNLGPEPEFFLFKLDEKGEPTLELNDDGGYFDLAPTDLGENCRRDIVLELEDMGFDIEASHHEVAPGQHEIDFKYADAVTACDNIQTFKLVVKTIARKHNLHATFMPKPLFGVNGSGMHFNVSLFKGKENAFFDPNTEMGLTETAYQFTAGVLKNARGFTAVCNPLVNSYKRLVPGYEAPCYIAWSGKNRSPLIRVPSSRGLSTRIEVRSVDPAANPYMALAAILEAGLDGIKNKLKVPEPVNQNIYEMNREEREAVGIQDLPSTLYTALKAMRENEVIKKALGNHIYNQFINSKSIEWDYYRTQVSEWERDQYMKQY

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulators: CodY, GlnR
    CodY (TF) important in Amino acid metabolism  RegPrecise
    GlnR (TF) important in Nitrogen assimilation  RegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]