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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01867
  • pan locus tag?: SAUPAN004428000
  • symbol: SAOUHSC_01867
  • pan gene symbol?: dat
  • synonym:
  • product: D-alanine aminotransferase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_01867
  • symbol: SAOUHSC_01867
  • product: D-alanine aminotransferase
  • replicon: chromosome
  • strand: -
  • coordinates: 1773873..1774721
  • length: 849
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGGAAAAAATTTTTTTAAATGGTGAGTTTGTAAGTCCAAGTGAAGCAAAGGTTTCATAC
    AACGACAGAGGATACGTATTTGGCGATGGTATTTATGAATACATTCGAGTATATAATGGT
    AAGTTATTTACAGTAACAGAACATTATGAAAGATTTTTACGTAGTGCCAATGAGATTGGT
    TTAGATTTAAATTATTCTGTAGAAGAATTAATTGAACTATCTCGTAAATTAGTTGATATG
    AATCAAATTGAAACTGGGGCAATTTATATTCAAGCAACGCGTGGTGTAGCTGAAAGGAAT
    CATAGCTTCCCGACACCTGAAGTAGAACCAGCAATTGTTGCTTATACAAAGAGTTATGAT
    CGTCCTTATGATCATTTAGAAAATGGTGTGAATGGTGTTACCGTTGAAGATATCCGATGG
    TTACGTTGCGACATTAAAAGCTTGAACTTATTAGGAAATGTATTAGCAAAAGAATATGCT
    GTGAAATATAATGCAGTTGAAGCAATTCAACATCGAGGTGAAACTGTAACTGAAGGATCT
    TCAAGTAATGCTTATGCAATTAAAGACGGTGTGATTTATACACATCCGATTAACAACTAT
    ATTCTTAATGGTATTACACGAATTGTAATTAAAAAAATTGCCGAAGACTATAACATCCCA
    TTTAAAGAAGAAACGTTTACTGTAGATTTCTTGAAAAACGCAGATGAAGTTATTGTTTCA
    AGTACTTCAGCTGAGGTTACACCTGTTATTAAATTAGATGGTGAACCAGTTAATGATGGT
    AAAGTTGGCCCAATTACACGTCAACTACAAGAAGGATTTGAAAAGTATATAGAGTCACAC
    AGTATTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    849

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_01867
  • symbol: SAOUHSC_01867
  • description: D-alanine aminotransferase
  • length: 282
  • theoretical pI: 4.68119
  • theoretical MW: 31893.6
  • GRAVY: -0.31773

Function[edit source | edit]

  • reaction:
    EC 2.6.1.21?  ExPASy
    D-amino-acid transaminaseD-alanine + 2-oxoglutarate = pyruvate + D-glutamate
  • TIGRFAM:
    MetabolismEnergy metabolismAmino acids and aminesD-amino-acid transaminase (TIGR01121; EC 2.6.1.21; HMM-score: 444.9)
    MetabolismAmino acid biosynthesisPyruvate familybranched-chain amino acid aminotransferase (TIGR01122; EC 2.6.1.42; HMM-score: 175.2)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersFolic acidaminodeoxychorismate lyase (TIGR03461; EC 4.1.3.38; HMM-score: 91.6)
    MetabolismAmino acid biosynthesisPyruvate familybranched-chain amino acid aminotransferase (TIGR01123; EC 2.6.1.42; HMM-score: 36.9)
  • TheSEED:  
    CarbohydratesCentral carbohydrate metabolismPyruvate Alanine Serine Interconversions D-alanine aminotransferase (EC 2.6.1.21) 
  • PFAM:
    no clan definedAminotran_4; Amino-transferase class IV (PF01063; HMM-score: 137.7)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors: pyridoxal 5'-phosphate
  • effectors:
  • protein partners:
    SAOUHSC_00943(ppnK)inorganic polyphosphate/ATP-NAD kinase  [1] (data from MRSA252)
    SAOUHSC_00679hypothetical protein  [1] (data from MRSA252)
    SAOUHSC_01316thermonuclease  [1] (data from MRSA252)

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.11
    • Ymax_pos: 22
    • Cmax: 0.13
    • Cmax_pos: 22
    • Smax: 0.115
    • Smax_pos: 16
    • Smean: 0.084
    • D: 0.1
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MEKIFLNGEFVSPSEAKVSYNDRGYVFGDGIYEYIRVYNGKLFTVTEHYERFLRSANEIGLDLNYSVEELIELSRKLVDMNQIETGAIYIQATRGVAERNHSFPTPEVEPAIVAYTKSYDRPYDHLENGVNGVTVEDIRWLRCDIKSLNLLGNVLAKEYAVKYNAVEAIQHRGETVTEGSSSNAYAIKDGVIYTHPINNYILNGITRIVIKKIAEDYNIPFKEETFTVDFLKNADEVIVSSTSAEVTPVIKLDGEPVNDGKVGPITRQLQEGFEKYIESHSI

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. 1.0 1.1 1.2 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)
  2. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-3661
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]