From AureoWiki
Jump to: navigation, search
NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01418
  • pan locus tag?: SAUPAN003831000
  • symbol: sucA
  • pan gene symbol?: sucA
  • synonym:
  • product: 2-oxoglutarate dehydrogenase E1 component

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_01418
  • symbol: sucA
  • product: 2-oxoglutarate dehydrogenase E1 component
  • replicon: chromosome
  • strand: -
  • coordinates: 1357199..1359997
  • length: 2799
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGACTAACGAAAGAAAAGAAGTTTCAGAGGCTCCTGTAAACTTCGGTGCGAATTTAGGT
    CTAATGTTAGATCTATATGATGACTTTTTACAAGATCCATCATCTGTACCAGAAGATTTA
    CAAGTCTTATTCAGCACAATTAAGAATGATGACTCAATTGTACCAGCTTTAAAAAGTACA
    AGTAGTCAAAATAGCGACGGCACAATTAAGCGTGTCATGCGTTTAATTGATAATATTCGC
    CAATACGGGCATCTTAAAGCCGATATTTATCCTGTAAATCCTCCAAAAAGGAAACATGTA
    CCTAAATTAGAGATTGAAGACTTTGATTTAGATCAACAGACTTTGGAAGGTATATCAGCA
    GGAATTGTTTCAGATCACTTTGCCGACATTTATGATAATGCTTATGAAGCAATTTTAAGA
    ATGGAAAAACGTTACAAAGGACCAATTGCATTTGAGTATACACATATTAATAACAATACC
    GAACGTGGTTGGTTAAAAAGAAGAATTGAAACGCCATATAAAGTAACGTTAAATAATAAC
    GAAAAAAGGGCACTATTCAAACAATTAGCGTATGTTGAAGGGTTTGAAAAATATCTTCAT
    AAAAACTTCGTTGGTGCAAAGCGTTTTTCAATTGAAGGGGTAGACGCACTTGTACCGATG
    TTACAACGTACTATTACGATTGCTGCGAAAGAAGGTATTAAAAATATACAAATAGGCATG
    GCTCACCGTGGACGTTTAAACGTTTTAACGCATGTCTTAGAAAAACCGTACGAAATGATG
    ATTTCAGAATTTATGCATACAGATCCAATGAAATTCTTACCTGAAGATGGTAGCTTGCAG
    TTAACTGCTGGATGGACTGGTGATGTGAAATATCACCTTGGTGGCATTAAAACTACTGAT
    TCATACGGTACAATGCAGCGTATTGCACTGGCTAACAATCCAAGTCACTTGGAAATTGTT
    GCACCTGTTGTTGAGGGGCGTACGAGAGCAGCACAAGATGATACACAACGAGCTGGGGCT
    CCGACGACTGATCATCATAAAGCAATGCCAATTATTATACATGGCGATGCTGCTTATCCT
    GGTCAAGGAATTAACTTCGAAACAATGAACTTAGGAAACTTGAAAGGCTATTCTACGGGT
    GGTTCATTGCATATTATTACTAACAATAGAATTGGATTTACTACAGAACCAATTGATGCA
    CGTTCAACAACTTATTCTACAGATGTGGCCAAAGGTTATGATGTGCCAATATTCCATGTC
    AATGCAGATGACGTTGAAGCTACTATTGAAGCAATTGATATTGCAATGGAATTTAGAAAA
    GAGTTTCATAAAGACGTCGTTATTGATTTAGTAGGTTATCGTCGTTTCGGACATAACGAA
    ATGGATGAACCATCAATTACTAATCCAGTTCCTTATCAGAATATTCGCAAACATGACTCT
    GTTGAATATGTGTTTGGTAAAAAGCTTGTTAATGAAGGTGTCATTTCAGAAGATGAAATG
    CATTCATTTATAGAACAAGTCCAAAAGGAACTAAGACAAGCTCATGATAAAATTAATAAA
    GCTGATAAAATGGATAATCCAGATATGGAAAAGCCTGCAGATCTTGCATTACCGTTACAA
    GCAGACGAACAATCATTTACTTTTGATCACTTGAAAGAAATAAATGATGCATTGTTAACA
    TATCCGGATGGCTTTAACATTTTGAAAAAGTTAAACAAAGTTCTTGAGAAGCGTCATGAG
    CCGTTTAATAAAGAAGATGGTTTAGTTGATTGGGCACAAGCAGAACAACTTGCATTTGCG
    ACAATTTTACAAGATGGTACACCGATTCGCTTAACTGGTCAAGATAGTGAACGTGGTACA
    TTCAGTCATAGGCATGCCGTGTTACATGATGAGCAAACAGGTGAAACATATACACCTTTA
    CATCATGTTCCTGATCAAAAAGCGACATTTGATATACACAATTCTCCGCTTTCAGAAGCA
    GCAGTAGTTGGTTTTGAATACGGCTATAATGTGGAAAACAAAAAAAGCTTCAATATTTGG
    GAAGCACAATATGGTGATTTTGCAAATATGTCACAAATGATTTTTGACAACTTCTTATTC
    AGTTCTCGCTCAAAATGGGGAGAACGTTCAGGATTAACATTATTCTTACCTCATGCATAT
    GAGGGTCAAGGGCCTGAACATTCATCAGCAAGATTAGAGCGATTTTTACAATTAGCTGCT
    GAAAATAATTGCACAGTTGTCAACTTATCTAGTTCAAGTAATTATTTCCACTTATTGCGT
    GCACAAGCGGCTAGTTTAGATTCTGAACAAATGCGACCATTGGTTGTTATGTCACCAAAA
    AGCTTACTGAGAAATAAAACAGTTGCAAAACCAATTGATGAATTTACTTCTGGTGGATTT
    GAGCCAATTTTGACAGAATCATATCAAGCGGATAAGGTTACAAAAGTTATTTTGGCAACT
    GGTAAAATGTTCATTGATTTAAAAGAAGCATTAGCTAAAAATCCAGACGAATCAGTATTA
    CTCGTTGCGATTGAAAGATTGTATCCATTCCCAGAGGAAGAGATTGAAGCATTACTAGCA
    CAATTGCCAAACCTTGAAGAAGTGTCATGGGTACAAGAAGAACCTAAAAATCAAGGTGCA
    TGGTTATATGTCTATCCATATGTTAAAGTGCTAGTTGCAGATAAATATGATTTAAGTTAT
    CATGGCAGAATTCAAAGGGCTGCTCCAGCTGAAGGCGATGGAGAAATTCATAAACTTGTT
    CAAAATAAAATTATAGAAAATGCATTAAAAAATAACTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2280
    2340
    2400
    2460
    2520
    2580
    2640
    2700
    2760
    2799

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_01418
  • symbol: SucA
  • description: 2-oxoglutarate dehydrogenase E1 component
  • length: 932
  • theoretical pI: 5.40096
  • theoretical MW: 105342
  • GRAVY: -0.469957

Function[edit source | edit]

  • reaction:
    EC 1.2.4.2?  ExPASy
    Oxoglutarate dehydrogenase (succinyl-transferring)2-oxoglutarate + [dihydrolipoyllysine-residue succinyltransferase] lipoyllysine = [dihydrolipoyllysine-residue succinyltransferase] S-succinyldihydrolipoyllysine + CO2
  • TIGRFAM:
    MetabolismEnergy metabolismTCA cycleoxoglutarate dehydrogenase (succinyl-transferring), E1 component (TIGR00239; EC 1.2.4.2; HMM-score: 1033.1)
    MetabolismEnergy metabolismPyruvate dehydrogenasepyruvate dehydrogenase (acetyl-transferring) E1 component, alpha subunit (TIGR03182; EC 1.2.4.1; HMM-score: 52.6)
    MetabolismEnergy metabolismPyruvate dehydrogenasepyruvate dehydrogenase (acetyl-transferring) E1 component, alpha subunit (TIGR03181; EC 1.2.4.1; HMM-score: 31.3)
  • TheSEED:  
    CarbohydratesCentral carbohydrate metabolismDehydrogenase complexes 2-oxoglutarate dehydrogenase E1 component (EC 1.2.4.2) 
    Central carbohydrate metabolismTCA Cycle 2-oxoglutarate dehydrogenase E1 component (EC 1.2.4.2) 
  • PFAM:
    THDP-binding (CL0254) E1_dh; Dehydrogenase E1 component (PF00676; HMM-score: 221.4)
    Transket_pyr; Transketolase, pyrimidine binding domain (PF02779; HMM-score: 180.2)
    TKC_like (CL0591) OxoGdeHyase_C; 2-oxoglutarate dehydrogenase C-terminal (PF16870; HMM-score: 117.1)
    no clan defined2-oxogl_dehyd_N; 2-oxoglutarate dehydrogenase N-terminus (PF16078; HMM-score: 20.7)
    T3SS_HrpK1; Type III secretion system translocator protein, HrpF (PF16937; HMM-score: 13.2)
    B_GA (CL0598) DUF1542; Domain of Unknown Function (DUF1542) (PF07564; HMM-score: 13.2)
    no clan definedDUF1840; Domain of unknown function (DUF1840) (PF08895; HMM-score: 12.7)

Structure, modifications & interactions[edit source | edit]

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.95
    • Cytoplasmic Membrane Score: 0.05
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.124
    • Ymax_pos: 11
    • Cmax: 0.111
    • Cmax_pos: 68
    • Smax: 0.196
    • Smax_pos: 1
    • Smean: 0.145
    • D: 0.132
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MTNERKEVSEAPVNFGANLGLMLDLYDDFLQDPSSVPEDLQVLFSTIKNDDSIVPALKSTSSQNSDGTIKRVMRLIDNIRQYGHLKADIYPVNPPKRKHVPKLEIEDFDLDQQTLEGISAGIVSDHFADIYDNAYEAILRMEKRYKGPIAFEYTHINNNTERGWLKRRIETPYKVTLNNNEKRALFKQLAYVEGFEKYLHKNFVGAKRFSIEGVDALVPMLQRTITIAAKEGIKNIQIGMAHRGRLNVLTHVLEKPYEMMISEFMHTDPMKFLPEDGSLQLTAGWTGDVKYHLGGIKTTDSYGTMQRIALANNPSHLEIVAPVVEGRTRAAQDDTQRAGAPTTDHHKAMPIIIHGDAAYPGQGINFETMNLGNLKGYSTGGSLHIITNNRIGFTTEPIDARSTTYSTDVAKGYDVPIFHVNADDVEATIEAIDIAMEFRKEFHKDVVIDLVGYRRFGHNEMDEPSITNPVPYQNIRKHDSVEYVFGKKLVNEGVISEDEMHSFIEQVQKELRQAHDKINKADKMDNPDMEKPADLALPLQADEQSFTFDHLKEINDALLTYPDGFNILKKLNKVLEKRHEPFNKEDGLVDWAQAEQLAFATILQDGTPIRLTGQDSERGTFSHRHAVLHDEQTGETYTPLHHVPDQKATFDIHNSPLSEAAVVGFEYGYNVENKKSFNIWEAQYGDFANMSQMIFDNFLFSSRSKWGERSGLTLFLPHAYEGQGPEHSSARLERFLQLAAENNCTVVNLSSSSNYFHLLRAQAASLDSEQMRPLVVMSPKSLLRNKTVAKPIDEFTSGGFEPILTESYQADKVTKVILATGKMFIDLKEALAKNPDESVLLVAIERLYPFPEEEIEALLAQLPNLEEVSWVQEEPKNQGAWLYVYPYVKVLVADKYDLSYHGRIQRAAPAEGDGEIHKLVQNKIIENALKNN

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 1.16 1.17 1.18 1.19 1.20 1.21 1.22 1.23 1.24 1.25 1.26 1.27 1.28 1.29 1.30 1.31 1.32 1.33 1.34 1.35 1.36 1.37 1.38 1.39 1.40 1.41 1.42 1.43 1.44 1.45 1.46 1.47 1.48 1.49 1.50 1.51 1.52 1.53 1.54 1.55 1.56 1.57 1.58 1.59 1.60 1.61 1.62 1.63 1.64 1.65 1.66 1.67 1.68 1.69 1.70 1.71 1.72 1.73 1.74 1.75 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)
  2. Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-3661
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  4. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]