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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00860
  • pan locus tag?: SAUPAN003021000
  • symbol: SAOUHSC_00860
  • pan gene symbol?:
  • synonym:
  • product: 5-nucleotidase family protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_00860
  • symbol: SAOUHSC_00860
  • product: 5-nucleotidase family protein
  • replicon: chromosome
  • strand: +
  • coordinates: 828214..829533
  • length: 1320
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    TTGAGGCTTACAATTTATCATACGAACGATATTCATAGTCATTTACATGAATACGAACGC
    ATTAAAGCATATATGGCAGAACATCGGCCACGACTTAATCATCCTTCTTTATATGTTGAT
    CTAGGTGATCATGTAGATTTATCCGCACCTATAACTGAAGCAACTTTAGGTAAAAAGAAT
    GTGGCATTACTAAATGAAGCAAAATGTGATGTTGCAACAATCGGTAATAATGAAGGGATG
    ACCATTTCATACGAAGCTTTAAATCACCTTTACGACGAAGCAAAATTTATAGTGACATGT
    AGCAATGTTATAGATGAATCAGGTCATTTACCAAATAATATCGTTTCTTCTTATATTAAG
    GACATAGACGGTGTGAAAATACTATTCGTTGCAGCGACAGCACCTTTTACCCCATTTTAT
    CGTGCACTAAATTGGATTGTTACCGATCCACTTGAATCTATAAAAGAAGAAATTGAACTT
    CAACGAGGTAAATTTGATGTATTAATCGTGCTAAGTCATTGTGGCATTTTCTTCGATGAA
    ACATTATGCCAAGAATTGCCTGAAATTGATGTCATTTTTGGTAGTCATACGCATCATTAT
    TTTGAACATGGTGAAATCAATAATGGTGTACTGATGGCGGCAGCTGGAAAGTATGGTAAT
    TATCTTGGAGAGGTTAATTTAACTTTTGAGGCACATAAAGTAGTACATAAAACTGCAAAG
    ATTATTCCTTTAGAAACATTACCTGAAGTTGAAACTTCATTTGAAGAAGAAGGAAAAACG
    TTAATGTCCAATTCAGTAATTCAACATCCAGTAGTGCTTAAGCGTAGTATGAATCACATA
    ACTGAAGCTGCATACTTATTAGCTCAAAGTGTTTGTGAGTATACACATGCACAATGTGCC
    ATCATCAATGCTGGCTTACTCGTTAAAGATATTGTAAAAGATGAAGTGACAGAATATGAC
    ATTCATCAAATGTTACCGCATCCGATTAATATGGTAAGGGTTAGACTTTTTGGTGTGAAA
    TTAAAAGAGATTATAGCTAAAAGTAATAAACAAGAATATATGTATGAACATGCACAAGGT
    TTGGGTTTCAGAGGGAATATATTTGGAGGATATATTCTTTATAATTTAGGGTACATTCAT
    TCTACAGGGCGTTACTATCTGAATGGAGAAGAAATCGAAGACGACAAAGAATATGTACTA
    GGTACGATAGATATGTATACGTTCGGTCGTTATTTCCCAACATTGAAAGAATTACCAAAA
    GAGTATTTAATGCCAGAGTTTTTAAGAGATATATTTAAAGAAAAATTATTGGAATATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_00860
  • symbol: SAOUHSC_00860
  • description: 5-nucleotidase family protein
  • length: 439
  • theoretical pI: 5.50128
  • theoretical MW: 50139
  • GRAVY: -0.156948

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    thiosulfohydrolase SoxB (TIGR04486; HMM-score: 64.3)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersPyridine nucleotidesNAD nucleotidase (TIGR01530; EC 3.1.3.5,3.6.1.22; HMM-score: 34.9)
    MetabolismTransport and binding proteinsOtherNAD nucleotidase (TIGR01530; EC 3.1.3.5,3.6.1.22; HMM-score: 34.9)
    MetabolismPurines, pyrimidines, nucleosides, and nucleotidesOther2',3'-cyclic-nucleotide 2'-phosphodiesterase (TIGR01390; EC 3.1.4.16; HMM-score: 11.7)
  • TheSEED:  
    Nucleosides and NucleotidesPurinesPurine conversions 5'-nucleotidase family protein in cluster with NagD-like phosphatase 
  • PFAM:
    Calcineurin (CL0163) Metallophos; Calcineurin-like phosphoesterase (PF00149; HMM-score: 42.7)
    no clan defined5_nucleotid_C; 5'-nucleotidase, C-terminal domain (PF02872; HMM-score: 30.7)
    Calcineurin (CL0163) PGA_cap; Bacterial capsule synthesis protein PGA_cap (PF09587; HMM-score: 14)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cellwall
    • Cytoplasmic Score: 0.11
    • Cytoplasmic Membrane Score: 0.02
    • Cellwall Score: 8.97
    • Extracellular Score: 0.9
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.103
    • Ymax_pos: 65
    • Cmax: 0.106
    • Cmax_pos: 65
    • Smax: 0.135
    • Smax_pos: 21
    • Smean: 0.086
    • D: 0.096
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MRLTIYHTNDIHSHLHEYERIKAYMAEHRPRLNHPSLYVDLGDHVDLSAPITEATLGKKNVALLNEAKCDVATIGNNEGMTISYEALNHLYDEAKFIVTCSNVIDESGHLPNNIVSSYIKDIDGVKILFVAATAPFTPFYRALNWIVTDPLESIKEEIELQRGKFDVLIVLSHCGIFFDETLCQELPEIDVIFGSHTHHYFEHGEINNGVLMAAAGKYGNYLGEVNLTFEAHKVVHKTAKIIPLETLPEVETSFEEEGKTLMSNSVIQHPVVLKRSMNHITEAAYLLAQSVCEYTHAQCAIINAGLLVKDIVKDEVTEYDIHQMLPHPINMVRVRLFGVKLKEIIAKSNKQEYMYEHAQGLGFRGNIFGGYILYNLGYIHSTGRYYLNGEEIEDDKEYVLGTIDMYTFGRYFPTLKELPKEYLMPEFLRDIFKEKLLEY

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-3661
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]