From AureoWiki
Jump to: navigation, search
NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00865
  • pan locus tag?: SAUPAN003027000
  • symbol: SAOUHSC_00865
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_00865
  • symbol: SAOUHSC_00865
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 831924..832703
  • length: 780
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGAAACAGTATAAAGCGTATTTAATCGATTTAGATGGCACAATGTATATGGGAACAGAT
    GAGATTGATGGAGCAAAACAATTCATCGATTATTTAAATGTAAAAGGCATTCCTCATTTA
    TACGTAACTAATAATTCAACAAAAACACCTGAGCAAGTAACTGAAAAATTACGTGAAATG
    CACATTGATGCTAAACCAGAAGAGGTTGTAACGTCAGCGTTAGCCACTGCTGATTATATT
    TCAGAACAATCACCAGGAGCATCAGTATATATGTTAGGTGGGAGTGGTTTAAATACTGCG
    TTAACCGAAGCGGGACTTGTCATTAAAAATGACGAGCATGTTGATTATGTAGTTATTGGA
    CTTGACGAACAAGTTACATATGAAAAGCTTGCGATTGCAACGTTAGGTGTAAGAAATGGT
    GCAACATTTATTTCTACAAATCCTGATGTATCAATTCCTAAAGAGCGTGGTTTATTACCT
    GGTAATGGTGCTATTACAAGTGTTGTAAGTGTATCGACAGGTGTATCGCCACAATTTATT
    GGTAAACCAGAACCGATTATTATGGTTAAAGCATTAGAAATTTTAGGATTAGATAAATCC
    GAAGTTGCTATGGTAGGCGATTTGTACGATACCGATATTATGTCTGGTATTAACGTAGGT
    ATGGATACGATTCATGTACAAACAGGTGTATCTACGTTAGAAGATGTGCAAAATAAAAAT
    GTGCCACCAACGTATTCTTTTAAAGATTTAAATGAAGCAATAGCTGAATTAGAAAAATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_00865
  • symbol: SAOUHSC_00865
  • description: hypothetical protein
  • length: 259
  • theoretical pI: 4.22967
  • theoretical MW: 27945.5
  • GRAVY: -0.0791506

Function[edit source | edit]

  • reaction:
    EC 3.1.3.-?  ExPASy
  • TIGRFAM:
    Unknown functionEnzymes of unknown specificityHAD hydrolase, TIGR01457 family (TIGR01457; HMM-score: 328.9)
    phosphoglycolate/pyridoxal phosphate phosphatase family (TIGR01452; EC 3.1.3.18; HMM-score: 145.3)
    Unknown functionEnzymes of unknown specificityHAD hydrolase, family IIA (TIGR01460; HMM-score: 133)
    Unknown functionEnzymes of unknown specificityHAD hydrolase, TIGR01458 family (TIGR01458; HMM-score: 75.7)
    HAD hydrolase, TIGR01459 family (TIGR01459; HMM-score: 59.6)
    HAD hydrolase, TIGR02253 family (TIGR02253; HMM-score: 40.9)
    noncanonical pyrimidine nucleotidase, YjjG family (TIGR02254; EC 3.1.3.5; HMM-score: 36.5)
    HAD phosphatase, family IIIA (TIGR01668; EC 3.1.3.-; HMM-score: 31.4)
    Unknown functionEnzymes of unknown specificityHAD hydrolase, family IIIA (TIGR01662; HMM-score: 27.2)
    HAD hydrolase, TIGR01456 family (TIGR01456; HMM-score: 21.6)
    histidinol-phosphate phosphatase domain (TIGR01656; HMM-score: 21.5)
    HAD hydrolase, REG-2-like, family IA (TIGR02252; HMM-score: 19.8)
    AHBA synthesis associated protein (TIGR01454; HMM-score: 19.5)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersPyridine nucleotides5'-nucleotidase, lipoprotein e(P4) family (TIGR01533; HMM-score: 19.3)
    MetabolismTransport and binding proteinsOther5'-nucleotidase, lipoprotein e(P4) family (TIGR01533; HMM-score: 19.3)
    pyrimidine 5'-nucleotidase (TIGR01993; EC 3.1.3.5; HMM-score: 17.4)
    Unknown functionEnzymes of unknown specificityHAD hydrolase, family IA, variant 1 (TIGR01549; HMM-score: 16.5)
    phosphoglycolate phosphatase, TA0175-type (TIGR01487; EC 3.1.3.18; HMM-score: 15.9)
    MetabolismEnergy metabolismSugarsphosphoglycolate phosphatase, bacterial (TIGR01449; EC 3.1.3.18; HMM-score: 15)
    Unknown functionEnzymes of unknown specificityHAD hydrolase, family IA, variant 3 (TIGR01509; HMM-score: 14.2)
    HAD hydrolase, TIGR01548 family (TIGR01548; HMM-score: 12.4)
    Unknown functionGeneralmannosyl-3-phosphoglycerate phosphatase homolog (TIGR02463; EC 3.1.3.-; HMM-score: 12)
  • TheSEED:  
    Hypothetical NagD-like phosphatase 
  • PFAM:
    HAD (CL0137) Hydrolase_6; haloacid dehalogenase-like hydrolase (PF13344; HMM-score: 87.9)
    Hydrolase_like; HAD-hyrolase-like (PF13242; HMM-score: 67)
    Hydrolase; haloacid dehalogenase-like hydrolase (PF00702; HMM-score: 46.8)
    HAD_2; haloacid dehalogenase-like hydrolase (PF13419; HMM-score: 42.8)
    Acid_phosphat_B; HAD superfamily, subfamily IIIB (Acid phosphatase) (PF03767; HMM-score: 11.8)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors: Mg2+
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.106
    • Ymax_pos: 29
    • Cmax: 0.114
    • Cmax_pos: 25
    • Smax: 0.17
    • Smax_pos: 22
    • Smean: 0.083
    • D: 0.097
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MKQYKAYLIDLDGTMYMGTDEIDGAKQFIDYLNVKGIPHLYVTNNSTKTPEQVTEKLREMHIDAKPEEVVTSALATADYISEQSPGASVYMLGGSGLNTALTEAGLVIKNDEHVDYVVIGLDEQVTYEKLAIATLGVRNGATFISTNPDVSIPKERGLLPGNGAITSVVSVSTGVSPQFIGKPEPIIMVKALEILGLDKSEVAMVGDLYDTDIMSGINVGMDTIHVQTGVSTLEDVQNKNVPPTYSFKDLNEAIAELEK

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  2. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]

Jakob Begun, Costi D Sifri, Samuel Goldman, Stephen B Calderwood, Frederick M Ausubel
Staphylococcus aureus virulence factors identified by using a high-throughput Caenorhabditis elegans-killing model.
Infect. Immun.: 2005, 73(2);872-7
[PubMed:15664928] [WorldCat.org] [DOI] (P p)