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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00945
  • pan locus tag?: SAUPAN003180000
  • symbol: SAOUHSC_00945
  • pan gene symbol?: mgtE
  • synonym:
  • product: magnesium transporter

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_00945
  • symbol: SAOUHSC_00945
  • product: magnesium transporter
  • replicon: chromosome
  • strand: +
  • coordinates: 916514..917899
  • length: 1386
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGTCAATGAACACAGATGAAAAAGAGCGTGTTCAAGAGGAATTATACGACCAAACTTTA
    TTAGATCAATATTTAGAAAATGATGATATTGATCAATTTAGAGATGAATTTCTAGCATTA
    CACACATATGAACAAAGTGAGTATTTTGAAGATACTACCGATGAAAATAGACAAAAGATT
    TTTCAATATTTATCACCTGAAGAAGTTGCAAATTTCTTTGATCAATTAGATATTGATGAC
    GATGAATATGAGTTGCTATTTGATAAGATGAATGCGACATACGCAAGTCACATATTAGAA
    GAAATGTCATACGACAATGCAGTAGATATTTTAAATGAGTTGACTAAACCAAAAGTTGCT
    AGTCTTTTAACATTGATGAATAAAGATGACGCGAATGAAATCAAAGCATTACTTCACTAT
    GATGAGGATACGGCCGGCGGTATTATGACGACGGAGTATTTATCACTTAAAGCGCATACG
    CCTGTTAAAGAAGCATTATTATTGGTCAAAGCGCAAGCACCAGACGCAGAAACAATATAT
    GTTATATTTGTCGTTGATGATGATGGTAAATTAGTAGGTGTTTTATCGCTAAGAGATTTA
    ATTGTAGCTGAAAATGATGCTTATATTGAAGATATTATGAATGAACGTGTCATTAGTGTG
    AATGTAGCAGACGACCAAGAAGATGTTGCTCAAGTTATGAGAGACTATGATTTCATGGCT
    GTACCTGTTATAGATTACCAAGAACATTTGCTTGGTATCATCACGATTGATGATATTTTA
    GACGTTATGGATGAAGAGGCTAGTGAAGACTACTCTCGTTTAGCCGGGGTATCAGATATC
    GATTCGACTAATGATTCAATCATTAAAACAGCATTAAAACGTTTACCATGGTTGATTATT
    TTAACATTTTTAGGAATGATTACTGCGACAATTTTAGGGAGATTCGAAAAAACATTAGAA
    AATGTAGCGCTACTCGCAGCGTTTATTCCTATTATTAGTGGTATGTCAGGAAATTCAGGT
    ACACAATCTTTAGCCGTTTCAGTTCGTAACATTACGACAGGGGAAATTAATGAGCAAAGT
    AAATTTAGAATTGCATTAAGAGAAGCAGGAAGTGGTGTATTATCGGGTGTTGTATGTTCA
    ACAATATTATTTACAATTATTGTTGCAATATATCATCAGCCACTTTTAGCATTAATCGTT
    GCAGGAAGTTTAACTTGTGCGATGACGGTGGGGACGTTTGTAGGTTCGATGATTCCATTA
    TTGATGAATAAATTAAATATCGATCCAGCAGTGGCTAGTGGACCATTTATTACAACAATT
    AATGATATTATTAGTATGTTGATTTATTTTGGTTTAGCTACATCATTTATGGCTTACTTA
    ATTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1386

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_00945
  • symbol: SAOUHSC_00945
  • description: magnesium transporter
  • length: 461
  • theoretical pI: 3.87276
  • theoretical MW: 51435.2
  • GRAVY: 0.13731

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    MetabolismTransport and binding proteinsCations and iron carrying compoundsmagnesium transporter (TIGR00400; HMM-score: 405.1)
    MetabolismPurines, pyrimidines, nucleosides, and nucleotidesPurine ribonucleotide biosynthesisinosine-5'-monophosphate dehydrogenase (TIGR01302; EC 1.1.1.205; HMM-score: 39.8)
    MetabolismTransport and binding proteinsAmino acids, peptides and aminesglycine betaine/L-proline transport ATP binding subunit (TIGR01186; HMM-score: 32.2)
    Unknown functionGeneralIMP dehydrogenase family protein (TIGR01303; HMM-score: 18.5)
    glutaredoxin-like protein, YruB-family (TIGR02196; HMM-score: 15.6)
    MetabolismEnergy metabolismSugarssugar isomerase, KpsF/GutQ family (TIGR00393; HMM-score: 13.5)
    MetabolismAmino acid biosynthesisSerine familycystathionine beta-synthase (TIGR01137; EC 4.2.1.22; HMM-score: 12.7)
  • TheSEED:  
    Mg/Co/Ni transporter MgtE / CBS domain 
  • PFAM:
    no clan definedMgtE; Divalent cation transporter (PF01769; HMM-score: 97.2)
    FliG (CL0436) MgtE_N; MgtE intracellular N domain (PF03448; HMM-score: 84.8)
    no clan definedCBS; CBS domain (PF00571; HMM-score: 72)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 4
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.097
    • Ymax_pos: 58
    • Cmax: 0.102
    • Cmax_pos: 12
    • Smax: 0.109
    • Smax_pos: 46
    • Smean: 0.075
    • D: 0.088
  • predicted transmembrane helices (TMHMM): 5

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MSMNTDEKERVQEELYDQTLLDQYLENDDIDQFRDEFLALHTYEQSEYFEDTTDENRQKIFQYLSPEEVANFFDQLDIDDDEYELLFDKMNATYASHILEEMSYDNAVDILNELTKPKVASLLTLMNKDDANEIKALLHYDEDTAGGIMTTEYLSLKAHTPVKEALLLVKAQAPDAETIYVIFVVDDDGKLVGVLSLRDLIVAENDAYIEDIMNERVISVNVADDQEDVAQVMRDYDFMAVPVIDYQEHLLGIITIDDILDVMDEEASEDYSRLAGVSDIDSTNDSIIKTALKRLPWLIILTFLGMITATILGRFEKTLENVALLAAFIPIISGMSGNSGTQSLAVSVRNITTGEINEQSKFRIALREAGSGVLSGVVCSTILFTIIVAIYHQPLLALIVAGSLTCAMTVGTFVGSMIPLLMNKLNIDPAVASGPFITTINDIISMLIYFGLATSFMAYLI

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-3661
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]