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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1013 [new locus tag: SACOL_RS05175 ]
  • pan locus tag?: SAUPAN003180000
  • symbol: mgtE
  • pan gene symbol?: mgtE
  • synonym:
  • product: magnesium transporter

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SACOL1013 [new locus tag: SACOL_RS05175 ]
  • symbol: mgtE
  • product: magnesium transporter
  • replicon: chromosome
  • strand: +
  • coordinates: 1020124..1021509
  • length: 1386
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGTCAATGAACACAGATGAAAAAGAGCGTGTTCAAGAGGAATTATACGACCAAACTTTA
    TTAGATCAATATTTAGAAAATGATGATATTGATCAATTTAGAGATGAATTTCTAGCATTA
    CACACATATGAACAAAGTGAGTATTTTGAAGATACTACCGATGAAAATAGACAAAAGATT
    TTTCAATATTTATCACCTGAAGAAGTTGCAAATTTCTTTGATCAATTAGATATTGATGAC
    GATGAATATGAGTTGCTATTTGATAAGATGAATGCGACATACGCAAGTCACATATTAGAA
    GAAATGTCATACGACAATGCAGTAGATATTTTAAATGAGTTGACTAAACCAAAAGTTGCT
    AGTCTTTTAACATTGATGAATAAAGATGACGCGAATGAAATCAAAGCATTACTTCACTAT
    GATGAGGATACGGCCGGCGGTATTATGACGACGGAGTATTTATCACTTAAAGCGCATACG
    CCTGTTAAAGAAGCATTATTATTGGTCAAAGCGCAAGCACCAGACGCAGAAACAATATAT
    GTTATATTTGTCGTTGATGATGATGGTAAATTAGTAGGTGTTTTATCGCTAAGAGATTTA
    ATTGTAGCTGAAAATGATGCTTATATTGAAGATATTATGAATGAACGTGTCATTAGTGTG
    AATGTAGCAGACGACCAAGAAGATGTTGCTCAAGTTATGAGAGACTATGATTTCATGGCT
    GTACCTGTTATAGATTACCAAGAACATTTGCTTGGTATCATCACGATTGATGATATTTTA
    GACGTTATGGATGAAGAGGCTAGTGAAGACTACTCTCGTTTAGCCGGGGTATCAGATATC
    GATTCGACTAATGATTCAATCATTAAAACAGCATTAAAACGTTTACCATGGTTGATTATT
    TTAACATTTTTAGGAATGATTACTGCGACAATTTTAGGGAGATTCGAAAAAACATTAGAA
    AATGTAGCGCTACTCGCAGCGTTTATTCCTATTATTAGTGGTATGTCAGGAAATTCAGGT
    ACACAATCTTTAGCCGTTTCAGTTCGTAACATTACGACAGGGGAAATTAATGAGCAAAGT
    AAATTTAGAATTGCATTAAGAGAAGCAGGAAGTGGTGTATTATCGGGTGTTGTATGTTCA
    ACAATATTATTTACAATTATTGTTGCAATATATCATCAGCCACTTTTAGCATTAATCGTT
    GCAGGAAGTTTAACTTGTGCGATGACGGTGGGGACGTTTGTAGGTTCGATGATTCCATTA
    TTGATGAATAAATTAAATATCGATCCAGCAGTGGCTAGTGGACCATTTATTACAACAATT
    AATGATATTATTAGTATGTTGATTTATTTTGGTTTAGCTACATCATTTATGGCTTACTTA
    ATTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1386

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SACOL1013 [new locus tag: SACOL_RS05175 ]
  • symbol: MgtE
  • description: magnesium transporter
  • length: 461
  • theoretical pI: 3.87276
  • theoretical MW: 51435.2
  • GRAVY: 0.13731

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    MetabolismTransport and binding proteinsCations and iron carrying compoundsmagnesium transporter (TIGR00400; HMM-score: 405.1)
    MetabolismPurines, pyrimidines, nucleosides, and nucleotidesPurine ribonucleotide biosynthesisinosine-5'-monophosphate dehydrogenase (TIGR01302; EC 1.1.1.205; HMM-score: 39.8)
    MetabolismTransport and binding proteinsAmino acids, peptides and aminesglycine betaine/L-proline transport ATP binding subunit (TIGR01186; HMM-score: 32.2)
    Unknown functionGeneralIMP dehydrogenase family protein (TIGR01303; HMM-score: 18.5)
    glutaredoxin-like protein, YruB-family (TIGR02196; HMM-score: 15.6)
    MetabolismEnergy metabolismSugarssugar isomerase, KpsF/GutQ family (TIGR00393; HMM-score: 13.5)
    MetabolismAmino acid biosynthesisSerine familycystathionine beta-synthase (TIGR01137; EC 4.2.1.22; HMM-score: 12.7)
  • TheSEED:  
    Mg/Co/Ni transporter MgtE / CBS domain 
  • PFAM:
    no clan definedMgtE; Divalent cation transporter (PF01769; HMM-score: 97.2)
    FliG (CL0436) MgtE_N; MgtE intracellular N domain (PF03448; HMM-score: 84.8)
    no clan definedCBS; CBS domain (PF00571; HMM-score: 72)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 4
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.097
    • Ymax_pos: 58
    • Cmax: 0.102
    • Cmax_pos: 12
    • Smax: 0.109
    • Smax_pos: 46
    • Smean: 0.075
    • D: 0.088
  • predicted transmembrane helices (TMHMM): 5

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MSMNTDEKERVQEELYDQTLLDQYLENDDIDQFRDEFLALHTYEQSEYFEDTTDENRQKIFQYLSPEEVANFFDQLDIDDDEYELLFDKMNATYASHILEEMSYDNAVDILNELTKPKVASLLTLMNKDDANEIKALLHYDEDTAGGIMTTEYLSLKAHTPVKEALLLVKAQAPDAETIYVIFVVDDDGKLVGVLSLRDLIVAENDAYIEDIMNERVISVNVADDQEDVAQVMRDYDFMAVPVIDYQEHLLGIITIDDILDVMDEEASEDYSRLAGVSDIDSTNDSIIKTALKRLPWLIILTFLGMITATILGRFEKTLENVALLAAFIPIISGMSGNSGTQSLAVSVRNITTGEINEQSKFRIALREAGSGVLSGVVCSTILFTIIVAIYHQPLLALIVAGSLTCAMTVGTFVGSMIPLLMNKLNIDPAVASGPFITTINDIISMLIYFGLATSFMAYLI

Experimental data[edit source | edit]

  • experimentally validated: PeptideAtlas
    experimental localization: Integral membrane [1] [2]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS ONE: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int. J. Med. Microbiol.: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

Relevant publications[edit source | edit]