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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00987
  • pan locus tag?: SAUPAN003250000
  • symbol: SAOUHSC_00987
  • pan gene symbol?: sspB
  • synonym:
  • product: cysteine protease

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_00987
  • symbol: SAOUHSC_00987
  • product: cysteine protease
  • replicon: chromosome
  • strand: -
  • coordinates: 958153..959334
  • length: 1182
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGAATAGTTCATGTAAATCTAGAGTATTCAATATTATAAGCATCATAATGGTTTCAATG
    CTTATTTTATCACTAGGCGCATTTGCTAATAACAATAAAGCCAAAGCCGATTCACACTCT
    AAACAGCTAGAAATTAATGTTAAGAGTGACAAAGTACCTCAAAAAGTAAAAGATCTAGCA
    CAACAACAATTTGCTGGTTATGCTAAAGCATTAGATAAACAAAGTAATGCAAAAACTGGT
    AAATATGAACTTGGCGAAGCTTTTAAAATTTATAAATTTAATGGTGAAGAAGATAATAGT
    TACTATTATCCAGTTATAAAAGACGGTAAAATTGTTTATACTTTAACACTTAGTCCTAAA
    AATAAAGATGATTTAAACAAATCAAAAGAAGACATGAATTACAGTGTTAAAATTTCAAAC
    TTCATCGCTAAAGATTTAGACCAAATTAAAGATAAAAATTCAAATATCACTGTTCTTACT
    GATGAAAAAGGGTTTTATTTTGAAGAAGATGGCAAAGTTAGATTAGTAAAAGCTACGCCT
    CTACCTGGTAATGTAAAAGAAAAAGAAAGTGCTAAAACAGTTTCAGCAAAATTGAAACAA
    GAGTTAAAAAATACAGTAACACCTACTAAAGTTGAAGAAAACGAAGCGATACAAGAAGAT
    CAAGTTCAATATGAAAATACATTAAAAAACTTCAAAATTAGAGAACAACAATTCGATAAC
    TCATGGTGTGCAGGATTCAGTATGGCAGCATTATTAAATGCAACTAAAAATACAGACACT
    TATAATGCACATGATATTATGCGTACATTATACCCTGAAGTAAGTGAGCAAGACCTTCCT
    AATTGTGCAACATTCCCTAATCAAATGATTGAATACGGTAAATCACAAGGCAGAGATATT
    CATTATCAAGAAGGCGTACCATCATATGAACAAGTTGATCAACTTACAAAAGATAATGTA
    GGAATTATGATCCTTGCACAAAGTGTATCTCAAAACCCTAATGACCCACATTTAGGACAT
    GCGCTAGCAGTTGTTGGTAATGCTAAAATTAATGACCAAGAAAAACTTATTTACTGGAAT
    CCTTGGGATACAGAATTATCAATCCAAGATGCAGATTCAAGCCTATTACATTTATCATTC
    AATCGTGATTATAACTGGTATGGTTCAATGATAGGTTACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1182

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_00987
  • symbol: SAOUHSC_00987
  • description: cysteine protease
  • length: 393
  • theoretical pI: 5.75782
  • theoretical MW: 44518.8
  • GRAVY: -0.655471

Function[edit source | edit]

  • reaction:
    EC 3.4.22.-?  ExPASy
  • TIGRFAM:
  • TheSEED:  
    Staphopain B precursor (EC 3.4.22.-) 
  • PFAM:
    Peptidase_CA (CL0125) Peptidase_C47; Staphopain peptidase C47 (PF05543; HMM-score: 316.3)
    Cystatin (CL0121) Staphopain_pro; Staphopain proregion (PF14731; HMM-score: 269.1)
    no clan definedNusG_II; NusG domain II (PF07009; HMM-score: 7.2)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Extracellular
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0
    • Extracellular Score: 10
    • Internal Helix: 1
  • LocateP: Secretory(released) (with CS)
    • Prediction by SwissProt Classification: Extracellular
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: 0.5
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: NKAKADSH
  • SignalP: Signal peptide length 37 aa
    • Ymax: 0.796
    • Ymax_pos: 37
    • Cmax: 0.826
    • Cmax_pos: 37
    • Smax: 0.925
    • Smax_pos: 23
    • Smean: 0.774
    • D: 0.788
  • predicted transmembrane helices (TMHMM): 1

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MNSSCKSRVFNIISIIMVSMLILSLGAFANNNKAKADSHSKQLEINVKSDKVPQKVKDLAQQQFAGYAKALDKQSNAKTGKYELGEAFKIYKFNGEEDNSYYYPVIKDGKIVYTLTLSPKNKDDLNKSKEDMNYSVKISNFIAKDLDQIKDKNSNITVLTDEKGFYFEEDGKVRLVKATPLPGNVKEKESAKTVSAKLKQELKNTVTPTKVEENEAIQEDQVQYENTLKNFKIREQQFDNSWCAGFSMAALLNATKNTDTYNAHDIMRTLYPEVSEQDLPNCATFPNQMIEYGKSQGRDIHYQEGVPSYEQVDQLTKDNVGIMILAQSVSQNPNDPHLGHALAVVGNAKINDQEKLIYWNPWDTELSIQDADSSLLHLSFNRDYNWYGSMIGY

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  2. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-3661
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]

J A Lindsay, S J Foster
Interactive regulatory pathways control virulence determinant production and stability in response to environmental conditions in Staphylococcus aureus.
Mol. Gen. Genet.: 1999, 262(2);323-31
[PubMed:10517329] [WorldCat.org] (P p)
K Rice, R Peralta, D Bast, J de Azavedo, M J McGavin
Description of staphylococcus serine protease (ssp) operon in Staphylococcus aureus and nonpolar inactivation of sspA-encoded serine protease.
Infect. Immun.: 2001, 69(1);159-69
[PubMed:11119502] [WorldCat.org] [DOI] (P p)
Isabella Massimi, Ellen Park, Kelly Rice, Werner Muller-Esterl, Daniel Sauder, Martin J McGavin
Identification of a novel maturation mechanism and restricted substrate specificity for the SspB cysteine protease of Staphylococcus aureus.
J. Biol. Chem.: 2002, 277(44);41770-7
[PubMed:12207024] [WorldCat.org] [DOI] (P p)
Lindsey Shaw, Ewa Golonka, Jan Potempa, Simon J Foster
The role and regulation of the extracellular proteases of Staphylococcus aureus.
Microbiology (Reading, Engl.): 2004, 150(Pt 1);217-28
[PubMed:14702415] [WorldCat.org] (P p)
Lindsey N Shaw, Ewa Golonka, Grzegorz Szmyd, Simon J Foster, James Travis, Jan Potempa
Cytoplasmic control of premature activation of a secreted protease zymogen: deletion of staphostatin B (SspC) in Staphylococcus aureus 8325-4 yields a profound pleiotropic phenotype.
J. Bacteriol.: 2005, 187(5);1751-62
[PubMed:15716447] [WorldCat.org] [DOI] (P p)