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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00998
  • pan locus tag?: SAUPAN003266000
  • symbol: SAOUHSC_00998
  • pan gene symbol?: fmtA
  • synonym:
  • product: methicillin resistance protein FmtA

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_00998
  • symbol: SAOUHSC_00998
  • product: methicillin resistance protein FmtA
  • replicon: chromosome
  • strand: +
  • coordinates: 970866..972059
  • length: 1194
  • essential: yes [1] DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGAAATTTAATAAAGTAAAACTAGTTATACATGCGTGTGTACTATTATTTATCATTATT
    TCTATAGCGTTAATTTTTCATCGATTACAGACGAAGACACATTCTATAGACCCAATACAT
    AAGGAAACAAAATTATCAGACAATGAAAAATATTTAGTGGATCGTAATAAGGAAAAGGTT
    GCGCCGTCTAAACTAAAAGAGGTATATAATAGCAAGGATCCTAAATATAAGAAAATTGAC
    AAGTATTTACAAAGTTCATTATTTAACGGTTCAGTAGCTATATATGAAAATGGCAAATTG
    AAAATGAGTAAAGGTTATGGATATCAAGATTTTGAAAAAGGTATTAAAAACACACCGAAT
    ACGATGTTTTTAATAGGTTCAGCTCAAAAATTTTCAACAGGGTTACTGTTAAAACAGTTA
    GAAGAAGAACATAAAATAAATATCAATGATCCAGTAAGTAAATACCTTCCATGGTTTAAA
    ACATCTAAGCCTATCCCATTGAAAGATTTAATGTTGCATCAAAGTGGATTATATAAATAT
    AAATCCTCAAAAGATTATAAAAATTTAGATCAAGCAGTTAAAGCGATTCAAAAACGTGGT
    ATTGATCCTAAGAAATACAAAAAGCATATGTATAACGATGGGAATTATTTAGTACTTGCG
    AAAGTAATTGAAGAAGTTACAGGTAAATCTTATGCTGAGAATTATTATACAAAAATAGGA
    GATCCTTTAAAACTTCAGCACACAGCATTTTATGATGAACAACCTTTTAAAAAATATCTA
    GCAAAAGGTTATGCTTATAATAGTACAGGACTTTCATTCTTAAGACCTAATATTTTGGAC
    CAATACTATGGTGCAGGTAATTTATATATGACACCAACAGATATGGGTAAATTAATTACT
    CAAATACAACAATATAAATTATTCAGTCCTAAAATAACCAATCCATTATTACATGAGTTT
    GGTACGAAAAAGTATCCAGATGAATATCGATATGGTTTCTATGCTAAGCCAACATTGAAT
    AGACTTAACGGGGGATTCTTTGGACAAGTCTTTACTGTTTACTATAATGATAAGTATGTA
    GTTGTACTTGCATTAAATGTAAAAGGAAACAATGAAGTTCGAATCAAACATATTTATAAT
    GATATTTTAAAACAAAATAAACCTTACAATACGAAGGGTGTTATTGTTCAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1194

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_00998
  • symbol: SAOUHSC_00998
  • description: methicillin resistance protein FmtA
  • length: 397
  • theoretical pI: 10.1143
  • theoretical MW: 46067.1
  • GRAVY: -0.561713

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
  • TheSEED:  
    Regulation and Cell signalingRegulation and Cell signaling - no subcategoryCell envelope-associated LytR-CpsA-Psr transcriptional attenuators FmtA protein involved in methicillin resistance 
  • PFAM:
    Beta-lactamase (CL0013) Beta-lactamase; Beta-lactamase (PF00144; HMM-score: 209.4)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helix: 1
  • LocateP: N-terminally anchored (No CS)
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: 0.5
    • N-terminally Anchored Score: 2
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.243
    • Ymax_pos: 36
    • Cmax: 0.148
    • Cmax_pos: 36
    • Smax: 0.669
    • Smax_pos: 5
    • Smean: 0.46
    • D: 0.327
  • predicted transmembrane helices (TMHMM): 1

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MKFNKVKLVIHACVLLFIIISIALIFHRLQTKTHSIDPIHKETKLSDNEKYLVDRNKEKVAPSKLKEVYNSKDPKYKKIDKYLQSSLFNGSVAIYENGKLKMSKGYGYQDFEKGIKNTPNTMFLIGSAQKFSTGLLLKQLEEEHKININDPVSKYLPWFKTSKPIPLKDLMLHQSGLYKYKSSKDYKNLDQAVKAIQKRGIDPKKYKKHMYNDGNYLVLAKVIEEVTGKSYAENYYTKIGDPLKLQHTAFYDEQPFKKYLAKGYAYNSTGLSFLRPNILDQYYGAGNLYMTPTDMGKLITQIQQYKLFSPKITNPLLHEFGTKKYPDEYRYGFYAKPTLNRLNGGFFGQVFTVYYNDKYVVVLALNVKGNNEVRIKHIYNDILKQNKPYNTKGVIVQ

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SAOUHSC_00998 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Roy R Chaudhuri, Andrew G Allen, Paul J Owen, Gil Shalom, Karl Stone, Marcus Harrison, Timothy A Burgis, Michael Lockyer, Jorge Garcia-Lara, Simon J Foster, Stephen J Pleasance, Sarah E Peters, Duncan J Maskell, Ian G Charles
    Comprehensive identification of essential Staphylococcus aureus genes using Transposon-Mediated Differential Hybridisation (TMDH).
    BMC Genomics: 2009, 10;291
    [PubMed:19570206] [WorldCat.org] [DOI] (I e)
  2. Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  4. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]

H Komatsuzawa, K Ohta, H Labischinski, M Sugai, H Suginaka
Characterization of fmtA, a gene that modulates the expression of methicillin resistance in Staphylococcus aureus.
Antimicrob. Agents Chemother.: 1999, 43(9);2121-5
[PubMed:10471551] [WorldCat.org] (P p)
H Komatsuzawa, M Sugai, K Ohta, T Fujiwara, S Nakashima, J Suzuki, C Y Lee, H Suginaka
Cloning and characterization of the fmt gene which affects the methicillin resistance level and autolysis in the presence of triton X-100 in methicillin-resistant Staphylococcus aureus.
Antimicrob. Agents Chemother.: 1997, 41(11);2355-61
[PubMed:9371333] [WorldCat.org] (P p)