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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01088
  • pan locus tag?: SAUPAN003367000
  • symbol: SAOUHSC_01088
  • pan gene symbol?: srtB
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_01088
  • symbol: SAOUHSC_01088
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 1049302..1050036
  • length: 735
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGAGAATGAAGCGATTTTTAACTATTGTACAAATTTTATTGGTTGTAATTATTATCATT
    TTTGGTTACAAAATTGTTCAAACATATATTGAAGACAAGCAAGAACGCGCAAATTATGAG
    AAATTACAACAAAAATTTCAAATGCTGATGAGCAAACATCAAGAACATGTGAGACCACAA
    TTTGAATCACTTGAAAAAATAAATAAAGACATTGTTGGATGGATAAAATTATCAGGAACA
    TCATTAAATTATCCAGTACTACAAGGTAAGACAAATCACGATTATTTAAATTTAGATTTT
    GAGCGAGAACATCGACGTAAAGGTAGTATTTTTATGGATTTTAGAAATGAATTGAAGAAT
    TTAAATCATAATACTATTTTATACGGGCACCATGTCGGTGATAATACGATGTTTGATGTG
    TTAGAAGATTATTTAAAGCAATCGTTTTATGAAAAACACAAGATAATTGAATTTGACAAT
    AAATATGGTAAATATCAATTGCAAGTATTTAGTGCATATAAAACTACTACTAAAGATAAT
    TACATACGTACAGATTTTGAAAATGATCAAGATTATCAACAATTTTTAGATGAGACAAAA
    CGTAAATCTGTAATTAATTCAGATGTTAATGTAACGGTAAAAGATAGAATAATGACTTTA
    TCAACGTGCGAAGATGCATATAGTGAAACGACGAAAAGAATTGTTGTTGTCGCAAAAATA
    ATTAAGGTAAGTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    735

Protein[edit source | edit]

Protein Data Bank: 4LFD

General[edit source | edit]

  • locus tag: SAOUHSC_01088
  • symbol: SAOUHSC_01088
  • description: hypothetical protein
  • length: 244
  • theoretical pI: 9.23648
  • theoretical MW: 29163.2
  • GRAVY: -0.582787

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Cell structureCell envelopeOthersortase, SrtB family (TIGR03064; EC 3.4.22.71; HMM-score: 304.4)
    Genetic information processingProtein fateProtein and peptide secretion and traffickingsortase, SrtB family (TIGR03064; EC 3.4.22.71; HMM-score: 304.4)
    Cell structureCell envelopeOthersortase (TIGR01076; EC 3.4.22.-; HMM-score: 10.4)
    Genetic information processingProtein fateProtein and peptide secretion and traffickingsortase (TIGR01076; EC 3.4.22.-; HMM-score: 10.4)
  • TheSEED:  
    Cell Wall and CapsuleGram-Positive cell wall componentsSortase NPQTN specific sortase B 
    Iron acquisition and metabolismIron acquisition and metabolism - no subcategoryHeme, hemin uptake and utilization systems in GramPositives NPQTN specific sortase B 
  • PFAM:
    no clan definedSortase; Sortase family (PF04203; HMM-score: 87)
    TPR (CL0020) TPR_21; Tetratricopeptide repeat-like domain (PF09976; HMM-score: 13.8)
    C_Lectin (CL0056) Ly49; Ly49-like protein, N-terminal region (PF08391; HMM-score: 10.5)
    no clan definedHJURP_C; Holliday junction regulator protein family C-terminal repeat (PF12347; HMM-score: 10.3)
    Arc_PepC_II; Archaeal Peptidase A24 C-terminus Type II (PF06847; HMM-score: 9.5)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helix: 1
  • LocateP: N-terminally anchored (No CS)
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: 0
    • N-terminally Anchored Score: 4
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.197
    • Ymax_pos: 11
    • Cmax: 0.169
    • Cmax_pos: 29
    • Smax: 0.509
    • Smax_pos: 1
    • Smean: 0.368
    • D: 0.264
  • predicted transmembrane helices (TMHMM): 1

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MRMKRFLTIVQILLVVIIIIFGYKIVQTYIEDKQERANYEKLQQKFQMLMSKHQEHVRPQFESLEKINKDIVGWIKLSGTSLNYPVLQGKTNHDYLNLDFEREHRRKGSIFMDFRNELKNLNHNTILYGHHVGDNTMFDVLEDYLKQSFYEKHKIIEFDNKYGKYQLQVFSAYKTTTKDNYIRTDFENDQDYQQFLDETKRKSVINSDVNVTVKDRIMTLSTCEDAYSETTKRIVVVAKIIKVS

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator: SAOUHSC_01592 (Fur*)
    Fur* (TF) important in Iron homeostasis

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-3661
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]