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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01154
  • pan locus tag?: SAUPAN003460000
  • symbol: SAOUHSC_01154
  • pan gene symbol?: sepF
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_01154
  • symbol: SAOUHSC_01154
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 1104514..1105077
  • length: 564
  • essential: yes [1] DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    GTGAGCCACTTGGCTTTAAAAGATTTATTTAGTGGATTTTTTGTAATAGATGATGAAGAG
    GAAGTAGAAGTACCTGACAAACAACAACAGGTAAATGAAGCGCCAGCAAAAGAGCAGTCA
    CAACAAACAACAAAACAAAACGCAATCAAATCAGTCCCTCAAAAATCTGCATCAAGATAT
    ACAACAACGTCAGAAGAAAGGAATAACCGTATGTCTAATTATTCAAAAAATAATTCACGT
    AATGTTGTAACTATGAACAATGCTACACCAAACAATGCATCACAAGAAAGTTCAAAAATG
    TGTTTATTCGAACCACGTGTTTTTTCAGATACACAAGATATTGCTGATGAGCTTAAAAAC
    CGCCGTGCGACACTTGTCAATTTACAACGTATTGATAAAGTATCAGCGAAAAGAATTATT
    GATTTTTTAAGCGGTACTGTTTATGCAATCGGTGGAGATATCCAACGTGTAGGTACTGAT
    ATTTTCTTATGTACGCCTGATAATGTGGAAGTAGCTGGAAGCATTACAGACCATATTGAA
    AATATGGAACATTCATTCGACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    564

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_01154
  • symbol: SAOUHSC_01154
  • description: hypothetical protein
  • length: 187
  • theoretical pI: 4.78677
  • theoretical MW: 21023.1
  • GRAVY: -0.705348

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    MetabolismEnergy metabolismTCA cycledihydrolipoyllysine-residue succinyltransferase, E2 component of oxoglutarate dehydrogenase (succinyl-transferring) complex (TIGR01347; EC 2.3.1.61; HMM-score: 11.3)
  • TheSEED:  
    Cell Division Cluster SepF, FtsZ-interacting protein related to cell division 
  • PFAM:
    no clan definedSepF; Cell division protein SepF (PF04472; HMM-score: 98.1)
    Ribosomal_L19e; Ribosomal protein L19e (PF01280; HMM-score: 13.6)
    PIN (CL0280) PIN_2; PIN domain (PF10130; HMM-score: 12.9)
    no clan definedSLC12; Solute carrier family 12 (PF03522; HMM-score: 12.3)
    Peptidase_AD (CL0130) Presenilin; Presenilin (PF01080; HMM-score: 4.9)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.141
    • Ymax_pos: 22
    • Cmax: 0.116
    • Cmax_pos: 37
    • Smax: 0.282
    • Smax_pos: 1
    • Smean: 0.157
    • D: 0.147
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MSHLALKDLFSGFFVIDDEEEVEVPDKQQQVNEAPAKEQSQQTTKQNAIKSVPQKSASRYTTTSEERNNRMSNYSKNNSRNVVTMNNATPNNASQESSKMCLFEPRVFSDTQDIADELKNRRATLVNLQRIDKVSAKRIIDFLSGTVYAIGGDIQRVGTDIFLCTPDNVEVAGSITDHIENMEHSFD

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Roy R Chaudhuri, Andrew G Allen, Paul J Owen, Gil Shalom, Karl Stone, Marcus Harrison, Timothy A Burgis, Michael Lockyer, Jorge Garcia-Lara, Simon J Foster, Stephen J Pleasance, Sarah E Peters, Duncan J Maskell, Ian G Charles
    Comprehensive identification of essential Staphylococcus aureus genes using Transposon-Mediated Differential Hybridisation (TMDH).
    BMC Genomics: 2009, 10;291
    [PubMed:19570206] [WorldCat.org] [DOI] (I e)
  2. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-3661
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]