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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01158
  • pan locus tag?: SAUPAN003463000
  • symbol: SAOUHSC_01158
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_01158
  • symbol: SAOUHSC_01158
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 1106292..1106909
  • length: 618
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGCCTTTTACACCAAATGAAATTAAGAATAAAGAGTTTTCACGTGTAAAGAATGGTTTA
    GAACCTACTGAAGTTGCTAATTTTTTGGAGCAACTAAGCACTGAAATTGAACGTCTTAAA
    GAAGATAAAAAACAACTTGAAAAAGTAATCGAAGAGAGAGATACTAATATTAAGTCTTAT
    CAAGACGTGCATCAATCTGTAAGTGATGCTTTGATACAAGCTCAAAAAGCTGGTGAAGAA
    ACTAAGCAAGCTGCAGAGAAACAAGCTGAAGCGATTATAGCTAAGGCAGAAGCGCAAGCT
    AATCAAATGGTTGGTGACGCGGTAGAAAAAGCACGCCGTTTAGCATTCCAGACTGAAGAT
    ATGAAACGTCAATCAAAAGTATTTAGATCGCGTTTCCGTATGTTAGTTGAAGCGCAATTA
    GACTTATTAAAAAACGAAGATTGGGATTACTTGTTGAATTATGATTTAGACGCTGAACAA
    GTGACGCTTGAAAATATTCATCATTTGCATGAAAATGATTTAAAGCCAGATGAAGTTGCA
    GCAAATGCACAAAATAATGCATCAAATACACCAGACAATAATCAACAATCCAATGATTCA
    GAAACAACTAAGAAGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    618

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_01158
  • symbol: SAOUHSC_01158
  • description: hypothetical protein
  • length: 205
  • theoretical pI: 4.63557
  • theoretical MW: 23513.8
  • GRAVY: -1.00683

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    DivIVA domain (TIGR03544; HMM-score: 29.9)
    Genetic information processingDNA metabolismOtherMutS2 family protein (TIGR01069; HMM-score: 12.7)
    Cellular processesCellular processesAdaptations to atypical conditionsphage shock protein B (TIGR02976; HMM-score: 10.8)
    MetabolismEnergy metabolismATP-proton motive force interconversionATP synthase F0, B subunit (TIGR01144; EC 3.6.3.14; HMM-score: 5.6)
    ATP synthase archaeal, H subunit (TIGR02926; EC 3.6.3.14; HMM-score: 4.9)
    SH3 domain protein (TIGR04211; HMM-score: 4.8)
  • TheSEED:  
    Cell Division and Cell CycleCell Division and Cell Cycle - no subcategoryBacterial Cytoskeleton Cell division initiation protein DivIVA 
    Bacterial Cell Division Cell division initiation protein DivIVA 
    Cell Division Cluster Cell division initiation protein DivIVA 
  • PFAM:
    no clan definedDivIVA; DivIVA protein (PF05103; HMM-score: 119.1)
    ATP_synthase (CL0255) ATP-synt_B; ATP synthase B/B' CF(0) (PF00430; HMM-score: 18.1)
    no clan definedLeu_zip; Leucine zipper (PF15294; HMM-score: 17.7)
    CDA (CL0109) APOBEC_N; APOBEC-like N-terminal domain (PF08210; HMM-score: 13.7)
    no clan definedDUF1664; Protein of unknown function (DUF1664) (PF07889; HMM-score: 12.1)
    HAD (CL0137) 5_nucleotid; 5' nucleotidase family (PF05761; HMM-score: 12)
    no clan definedDUF2570; Protein of unknown function (DUF2570) (PF10828; HMM-score: 11.7)
    ISG65-75; Invariant surface glycoprotein (PF11727; HMM-score: 11.6)
    FlaC_arch; Flagella accessory protein C (FlaC) (PF05377; HMM-score: 10.9)
    KELK; KELK-motif containing domain of MRCK Ser/Thr protein kinase (PF15796; HMM-score: 10.6)
    ZYG-11_interact; Interactor of ZYG-11 (PF05884; HMM-score: 9.5)
    Dak2; DAK2 domain (PF02734; HMM-score: 9.5)
    CENP-F_leu_zip; Leucine-rich repeats of kinetochore protein Cenp-F/LEK1 (PF10473; HMM-score: 9.1)
    DUF349; Domain of Unknown Function (DUF349) (PF03993; HMM-score: 8.7)
    YjbJ-CsbD-like (CL0406) DUF883; Bacterial protein of unknown function (DUF883) (PF05957; HMM-score: 7.8)
    no clan definedYtxH; YtxH-like protein (PF12732; HMM-score: 7.6)
    NADP_Rossmann (CL0063) NAD_binding_5; Myo-inositol-1-phosphate synthase (PF07994; HMM-score: 7.3)
    no clan definedLMBR1; LMBR1-like membrane protein (PF04791; HMM-score: 6.6)
    TMPIT; TMPIT-like protein (PF07851; HMM-score: 6.2)
    DASH_Spc19; Spc19 (PF08287; HMM-score: 6.1)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.153
    • Ymax_pos: 11
    • Cmax: 0.107
    • Cmax_pos: 42
    • Smax: 0.334
    • Smax_pos: 4
    • Smean: 0.238
    • D: 0.186
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MPFTPNEIKNKEFSRVKNGLEPTEVANFLEQLSTEIERLKEDKKQLEKVIEERDTNIKSYQDVHQSVSDALIQAQKAGEETKQAAEKQAEAIIAKAEAQANQMVGDAVEKARRLAFQTEDMKRQSKVFRSRFRMLVEAQLDLLKNEDWDYLLNYDLDAEQVTLENIHHLHENDLKPDEVAANAQNNASNTPDNNQQSNDSETTKK

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  2. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]