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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01188
  • pan locus tag?: SAUPAN003504000
  • symbol: SAOUHSC_01188
  • pan gene symbol?: rsgA
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_01188
  • symbol: SAOUHSC_01188
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 1140425..1141300
  • length: 876
  • essential: yes [1] DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    TTGAAGACAGGTCGAATAGTGAAATCAATTAGTGGGGTATATCAAGTAGACGTTAATGGC
    GAACGTTTCAATACAAAACCACGAGGATTATTTAGAAAGAAAAAATTTTCACCGGTAGTT
    GGTGATATAGTGGAATTTGAAGTTCAAAACATTAACGAAGGTTATATTCATCAAGTGTTT
    GAGCGGGAAAATGAGTTGAAAAGACCACCTGTAAGTAATATAGATACACTAGTAATTGTA
    ATGAGTGCTGTCGAGCCAAATTTTTCAACGCAATTATTAGATCGATTTTTAGTTATTGCA
    CATTCGTATCAGTTAAATGCGAGAATTTTGGTGACTAAAAAAGATAAAACACCAATTGAA
    AAGCAGTTCGAAATAAATGAGTTGTTGAAAATATATGAAAATATTGGCTATGAGACTGAA
    TTTATTGGAAATGATGATGATCGAAAAAAAATTGTAGAAGCTTGGCCAGCTGGACTTATA
    GTACTTAGTGGTCAATCAGGTGTCGGTAAGTCCACTTTCTTAAATCATTATCGTCCAGAA
    CTTAATCTTGAGACAAATGATATATCAAAATCATTAAATCGAGGAAAGCATACTACAAGA
    CATGTCGAACTATTCGAACGTCAAAACGGTTATATTGCAGACACACCTGGATTCAGTGCT
    TTAGATTTTGATCATATAGATAAAGATGAAATAAAAGATTATTTTCTTGAATTAAATCGA
    TATGGTGAAACATGTAAGTTTAGGAATTGTAATCATATCAAAGAACCTAATTGTAATGTT
    AAGCATCAATTAGAGATAGGGAATATTGCGCAATTTAGATACGACCATTATTTACAACTA
    TTTAATGAAATTTCAAATAGAAAGGTTAGATATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    876

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_01188
  • symbol: SAOUHSC_01188
  • description: hypothetical protein
  • length: 291
  • theoretical pI: 7.68091
  • theoretical MW: 33890.2
  • GRAVY: -0.578007

Function[edit source | edit]

  • reaction:
    EC 3.6.1.-?  ExPASy
  • TIGRFAM:
    Genetic information processingProtein synthesisTranslation factorsribosome small subunit-dependent GTPase A (TIGR00157; EC 3.6.-.-; HMM-score: 245.9)
    Genetic information processingProtein synthesisOtherribosome biogenesis GTP-binding protein YsxC (TIGR03598; HMM-score: 23.9)
    Unknown functionGeneralsmall GTP-binding protein domain (TIGR00231; HMM-score: 17)
    Genetic information processingProtein synthesisOtherribosome biogenesis GTPase YqeH (TIGR03597; HMM-score: 15.3)
    MetabolismPurines, pyrimidines, nucleosides, and nucleotidesNucleotide and nucleoside interconversionsguanylate kinase (TIGR03263; EC 2.7.4.8; HMM-score: 12.8)
    Cellular processesCellular processesToxin production and resistanceputative bacteriocin export ABC transporter, lactococcin 972 group (TIGR03608; HMM-score: 12.3)
    MetabolismTransport and binding proteinsUnknown substrateputative bacteriocin export ABC transporter, lactococcin 972 group (TIGR03608; HMM-score: 12.3)
  • TheSEED:  
    Protein MetabolismProtein biosynthesisUniversal GTPases Ribosome small subunit-stimulated GTPase EngC 
    A Gram-positive cluster that relates ribosomal protein L28P to a set of uncharacterized proteins Ribosome small subunit-stimulated GTPase EngC 
  • PFAM:
    P-loop_NTPase (CL0023) RsgA_GTPase; RsgA GTPase (PF03193; HMM-score: 179.7)
    OB (CL0021) RsgA_N; RsgA N-terminal domain (PF16745; HMM-score: 70)
    P-loop_NTPase (CL0023) MMR_HSR1; 50S ribosome-binding GTPase (PF01926; HMM-score: 24.9)
    AAA_22; AAA domain (PF13401; HMM-score: 17.3)
    AAA_29; P-loop containing region of AAA domain (PF13555; HMM-score: 16.6)
    AAA_16; AAA ATPase domain (PF13191; HMM-score: 15.8)
    ABC_tran; ABC transporter (PF00005; HMM-score: 15.1)
    RNA_helicase; RNA helicase (PF00910; HMM-score: 14.5)
    AAA_33; AAA domain (PF13671; HMM-score: 14.3)
    AAA_18; AAA domain (PF13238; HMM-score: 14.2)
    KAP_NTPase; KAP family P-loop domain (PF07693; HMM-score: 13.7)
    AAA_24; AAA domain (PF13479; HMM-score: 13.6)
    AAA_23; AAA domain (PF13476; HMM-score: 13.4)
    AAA_25; AAA domain (PF13481; HMM-score: 13.2)
    PRK; Phosphoribulokinase / Uridine kinase family (PF00485; HMM-score: 12.2)
    NACHT; NACHT domain (PF05729; HMM-score: 11.6)
    T2SSE; Type II/IV secretion system protein (PF00437; HMM-score: 11.2)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors: Zn2+
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.115
    • Ymax_pos: 24
    • Cmax: 0.111
    • Cmax_pos: 24
    • Smax: 0.199
    • Smax_pos: 1
    • Smean: 0.115
    • D: 0.115
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MKTGRIVKSISGVYQVDVNGERFNTKPRGLFRKKKFSPVVGDIVEFEVQNINEGYIHQVFERENELKRPPVSNIDTLVIVMSAVEPNFSTQLLDRFLVIAHSYQLNARILVTKKDKTPIEKQFEINELLKIYENIGYETEFIGNDDDRKKIVEAWPAGLIVLSGQSGVGKSTFLNHYRPELNLETNDISKSLNRGKHTTRHVELFERQNGYIADTPGFSALDFDHIDKDEIKDYFLELNRYGETCKFRNCNHIKEPNCNVKHQLEIGNIAQFRYDHYLQLFNEISNRKVRY

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Roy R Chaudhuri, Andrew G Allen, Paul J Owen, Gil Shalom, Karl Stone, Marcus Harrison, Timothy A Burgis, Michael Lockyer, Jorge Garcia-Lara, Simon J Foster, Stephen J Pleasance, Sarah E Peters, Duncan J Maskell, Ian G Charles
    Comprehensive identification of essential Staphylococcus aureus genes using Transposon-Mediated Differential Hybridisation (TMDH).
    BMC Genomics: 2009, 10;291
    [PubMed:19570206] [WorldCat.org] [DOI] (I e)
  2. Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-3661
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  4. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

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