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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01272
  • pan locus tag?: SAUPAN003600000
  • symbol: SAOUHSC_01272
  • pan gene symbol?: mutS
  • synonym:
  • product: DNA mismatch repair protein MutS

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_01272
  • symbol: SAOUHSC_01272
  • product: DNA mismatch repair protein MutS
  • replicon: chromosome
  • strand: +
  • coordinates: 1226777..1229299
  • length: 2523
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGTTTTATGAAGATGCCAAGGAGGCATCACGTGTACTTGAAATTACTTTAACTAAAAGA
    GATGCTAAAAAAGAAAATCCAATTCCGATGTGTGGTGTTCCGTATCATTCTGCAGATAGT
    TATATAGATACACTTGTTAATAATGGATATAAAGTAGCTATTTGTGAACAGATGGAAGAT
    CCGAAACAAACGAAAGGTATGGTTAGACGTGAGGTAGTAAGAATTGTGACTCCAGGAACT
    GTGATGGAGCAAGGTGGTGTAGATGATAAACAAAATAACTATATTTTAAGTTTTGTTATG
    AATCAACCTGAAATTGCGCTTAGTTACTGTGATGTTTCTACTGGCGAATTAAAGGTTACA
    CATTTTAATGATGAAGCGACTTTATTAAATGAAATTACGACGATAAACCCTAACGAAGTT
    GTTATCAATGACAATATTTCCGATAATTTAAAAAGACAAATTAATATGGTGACAGAAACA
    ATAACAGTCAGGGAAACGTTATCATCAGAAATCTATAGTGTGAATCAAACTGAACATAAA
    TTAATGTATCAAGCGACACAATTATTGCTAGATTATATTCATCATACACAAAAACGTGAT
    TTATCGCATATCGAGGATGTTGTTCAATATGCAGCTATAGATTATATGAAAATGGATTTT
    TATGCTAAGAGAAACCTTGAGTTAACGGAAAGCATTCGATTAAAATCAAAAAAAGGAACG
    CTACTTTGGCTAATGGACGAAACGAAAACACCAATGGGAGCACGCCGCTTAAAACAATGG
    ATAGATAGACCACTAATAAGTAAAGAACAAATTGAAGCACGATTAGATATCGTTGATGAA
    TTTAGTGCTCATTTCATAGAAAGAGACACCTTAAGAACATATCTTAATCAAGTGTATGAT
    ATTGAACGTCTTGTTGGGCGTGTTAGTTACGGAAATGTTAATGCGAGAGATTTAATTCAA
    CTTAAACATTCCATTTCTGAAATACCGAATATTAAAGCATTACTAAATTCTATGAATCAG
    AATACTCTTGTACAAGTTAATCAACTAGAACCCCTTGATGATTTACTTGATATATTAGAA
    CAGAGTTTAGTAGAAGAACCACCAATTTCAGTTAAAGATGGCGGACTATTCAAAGTTGGT
    TTTAATACGCAATTAGATGAATATCTTGAAGCTTCAAAAAACGGAAAAACATGGTTAGCA
    GAATTACAAGCCAAAGAAAGACAACGTACAGGAATAAAATCATTGAAAATAAGCTTTAAT
    AAAGTGTTTGGTTATTTTATAGAAATAACACGTGCCAACTTGCAAAATTTTGAACCAAGT
    GAATTTGGTTATATGAGGAAGCAAACGTTATCGAATGCTGAACGTTTTATAACTGATGAA
    CTTAAAGAAAAAGAAGATATCATTTTAGGTGCGGAAGACAAAGCCATCGAATTAGAATAT
    CAATTATTTGTTCAGCTACGTGAAGAAGTTAAAAAATATACTGAACGTTTACAACAACAA
    GCTAAAATTATTTCAGAGCTAGATTGTTTACAGAGCTTTGCAGAAATTGCTCAAAAATAT
    AATTACACTAGGCCTTCATTTAGTGAAAATAAAACATTAGAATTAGTGGAATCTAGGCAC
    CCAGTAGTGGAAAGAGTAATGGATTATAATGACTATGTGCCTAATAATTGTCGATTAGAT
    AATGAAACATTTATATATTTAATTACAGGTCCGAATATGTCTGGTAAATCGACATATATG
    AGACAAGTTGCCATAATTAGTATAATGGCCCAAATGGGAGCTTATGTCCCTTGTAAAGAG
    GCAGTGTTACCTATATTTGATCAAATATTCACTAGAATAGGTGCGGCAGATGATTTGGTT
    TCAGGTAAGAGTACGTTTATGGTAGAAATGCTAGAAGCACAAAAGGCATTAACTTATGCA
    ACAGAGGATAGTTTGATTATTTTCGATGAAATTGGACGTGGTACTTCAACGTATGACGGT
    TTAGCTTTAGCGCAGGCAATGATAGAGTATGTAGCTGAAACATCACATGCTAAAACGTTA
    TTTTCAACACATTATCATGAATTGACAACATTAGATCAAGCATTACCAAGTCTAAAAAAT
    GTTCACGTCGCTGCTAATGAATATAAAGGTGAACTTATATTCTTGCATAAAGTCAAAGAT
    GGTGCAGTTGACGATAGTTATGGTATTCAAGTTGCGAAATTAGCTGATTTACCTGAAAAA
    GTTATTAGCAGAGCACAAGTGATTCTAAGCGAGTTTGAAGCGTCTGCTGGTAAAAAATCA
    TCGATATCAAATTTAAAAATGGTCGAAAATGAACCTGAAATTAATCAAGAAAATTTAAAC
    TTAAGTGTTGAAGAAACAACTGATACTTTATCTCAAAAAGACTTTGAACAAGCATCATTT
    GATTTGTTTGAAAATGATCAAAAAAGCGAGATTGAACTACAAATTAAAAATTTGAATTTA
    TCTAATATGACACCAATTGAGGCATTGGTGAAGTTAAGTGAATTACAAAATCAATTAAAA
    TAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2280
    2340
    2400
    2460
    2520
    2523

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_01272
  • symbol: SAOUHSC_01272
  • description: DNA mismatch repair protein MutS
  • length: 840
  • theoretical pI: 4.67212
  • theoretical MW: 95980.5
  • GRAVY: -0.38131

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Genetic information processingDNA metabolismDNA replication, recombination, and repairDNA mismatch repair protein MutS (TIGR01070; HMM-score: 1026)
    Genetic information processingDNA metabolismOtherMutS2 family protein (TIGR01069; HMM-score: 147.9)
  • TheSEED:  
    DNA MetabolismDNA repairDNA repair, bacterial MutL-MutS system DNA mismatch repair protein MutS 
  • PFAM:
    P-loop_NTPase (CL0023) MutS_V; MutS domain V (PF00488; HMM-score: 275.7)
    no clan definedMutS_III; MutS domain III (PF05192; HMM-score: 151.3)
    MutS_I; MutS domain I (PF01624; HMM-score: 107.3)
    MutS_IV; MutS family domain IV (PF05190; HMM-score: 96.6)
    MutS_II; MutS domain II (PF05188; HMM-score: 65.1)
    P-loop_NTPase (CL0023) AAA_29; P-loop containing region of AAA domain (PF13555; HMM-score: 17.5)
    AAA_14; AAA domain (PF13173; HMM-score: 17.1)
    ATPase_2; ATPase domain predominantly from Archaea (PF01637; HMM-score: 11.3)
    AAA_23; AAA domain (PF13476; HMM-score: 9.9)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.114
    • Ymax_pos: 25
    • Cmax: 0.123
    • Cmax_pos: 25
    • Smax: 0.184
    • Smax_pos: 2
    • Smean: 0.106
    • D: 0.111
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MFYEDAKEASRVLEITLTKRDAKKENPIPMCGVPYHSADSYIDTLVNNGYKVAICEQMEDPKQTKGMVRREVVRIVTPGTVMEQGGVDDKQNNYILSFVMNQPEIALSYCDVSTGELKVTHFNDEATLLNEITTINPNEVVINDNISDNLKRQINMVTETITVRETLSSEIYSVNQTEHKLMYQATQLLLDYIHHTQKRDLSHIEDVVQYAAIDYMKMDFYAKRNLELTESIRLKSKKGTLLWLMDETKTPMGARRLKQWIDRPLISKEQIEARLDIVDEFSAHFIERDTLRTYLNQVYDIERLVGRVSYGNVNARDLIQLKHSISEIPNIKALLNSMNQNTLVQVNQLEPLDDLLDILEQSLVEEPPISVKDGGLFKVGFNTQLDEYLEASKNGKTWLAELQAKERQRTGIKSLKISFNKVFGYFIEITRANLQNFEPSEFGYMRKQTLSNAERFITDELKEKEDIILGAEDKAIELEYQLFVQLREEVKKYTERLQQQAKIISELDCLQSFAEIAQKYNYTRPSFSENKTLELVESRHPVVERVMDYNDYVPNNCRLDNETFIYLITGPNMSGKSTYMRQVAIISIMAQMGAYVPCKEAVLPIFDQIFTRIGAADDLVSGKSTFMVEMLEAQKALTYATEDSLIIFDEIGRGTSTYDGLALAQAMIEYVAETSHAKTLFSTHYHELTTLDQALPSLKNVHVAANEYKGELIFLHKVKDGAVDDSYGIQVAKLADLPEKVISRAQVILSEFEASAGKKSSISNLKMVENEPEINQENLNLSVEETTDTLSQKDFEQASFDLFENDQKSEIELQIKNLNLSNMTPIEALVKLSELQNQLK

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-3661
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]

Franziska Schaaff, Andrea Reipert, Gabriele Bierbaum
An elevated mutation frequency favors development of vancomycin resistance in Staphylococcus aureus.
Antimicrob. Agents Chemother.: 2002, 46(11);3540-8
[PubMed:12384362] [WorldCat.org] (P p)