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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01279
  • pan locus tag?: SAUPAN003615000
  • symbol: SAOUHSC_01279
  • pan gene symbol?:
  • synonym:
  • product: hydrolase alpha/beta fold domain-containing protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_01279
  • symbol: SAOUHSC_01279
  • product: hydrolase alpha/beta fold domain-containing protein
  • replicon: chromosome
  • strand: +
  • coordinates: 1236758..1237672
  • length: 915
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGACTGAAAAACAATTTAAATTAACTGTACAAGATAATACGAATATTGAAGTTAAAGTG
    AATTTTACAGATGTAGATTCAAAAGGAATTATTCATATATTTCATGGTATGGCTGAACAT
    ATGGAACGTTACGATAAATTAGCACATGCACTTTCAAAGCATGGCTTCGATGTGATACGT
    CATAATCATCGAGGACATGGTATTAATATTGATGAATCAACAAGAGGGCATTACGATGAT
    ATGAAACGAGTTATCGGTGATGCCTTTGAAGTAGCGCAAACAGTGAGAGGCAATGTTGAT
    AAACCATACATTATAATCGGACATTCAATGGGATCCGTTATAGCTAGATTGTTTGTAGAA
    ACATATCCGCAATATGTTGATGGTCTAATTTTAAGTGGTACTGGTATGTATTCATTATGG
    AAAGGTTTACCAACCGTTAAAGTGTTACAACTGATTACAAAAATTTATGGTGCTGAGAAA
    CGAGTTGAATGGGTTAACCAGTTAGTATCAAATAGTTTTAATAAAAAAATACGTCCATTA
    CGTACACAAAGTGATTGGATTTCTAGTAATCCAATTGAAGTAGATAACTTTATTAAAGAT
    CCATATAGTGGATTTAATGTGTCAAATCAATTATTATATCAAACAGCCTATTATATGCTA
    CATACATCACAATTAAAAAATATGAAAATGTTAAATCATGCCATGCCTATATTATTAGTT
    TCAGGATATGACGATCCTTTAGGTGATTATGGTAAAGGGATTTTAAAATTGGCGAATATA
    TATAGAAAAGCTGGCATTAAAAATGTTAAAGTGAATCTTTATCATCATAAACGTCATGAA
    GTGTTATTTGAAAAAGATCATGACAAAATTTGGGAAGACTTGTTTAAATGGTTGAATCAA
    TTTTATAAAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    915

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_01279
  • symbol: SAOUHSC_01279
  • description: hydrolase alpha/beta fold domain-containing protein
  • length: 304
  • theoretical pI: 9.65305
  • theoretical MW: 35255.3
  • GRAVY: -0.419408

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersMenaquinone and ubiquinone2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase (TIGR03695; EC 4.2.99.20; HMM-score: 35.4)
    MetabolismEnergy metabolismOther3-oxoadipate enol-lactonase (TIGR02427; EC 3.1.1.24; HMM-score: 25.2)
    2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase (TIGR03343; EC 3.7.1.-; HMM-score: 24.1)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersBiotinpimelyl-[acyl-carrier protein] methyl ester esterase (TIGR01738; EC 3.1.1.85; HMM-score: 23.3)
    proline-specific peptidase (TIGR01250; HMM-score: 22.9)
    MetabolismEnergy metabolismPhotosynthesisputative magnesium chelatase accessory protein (TIGR03056; HMM-score: 14.3)
  • TheSEED:  
    Fatty Acids, Lipids, and IsoprenoidsTriacylglycerolsTriacylglycerol metabolism Lysophospholipase (EC 3.1.1.5) 
    TriacylglycerolsTriacylglycerol metabolism Monoglyceride lipase (EC 3.1.1.23) 
    Triacylglycerol metabolism putative 
  • PFAM:
    AB_hydrolase (CL0028) Hydrolase_4; Serine aminopeptidase, S33 (PF12146; HMM-score: 170.4)
    Abhydrolase_1; alpha/beta hydrolase fold (PF00561; HMM-score: 72.4)
    Abhydrolase_6; Alpha/beta hydrolase family (PF12697; HMM-score: 29.3)
    Abhydrolase_5; Alpha/beta hydrolase family (PF12695; HMM-score: 18.7)
    Peptidase_S28; Serine carboxypeptidase S28 (PF05577; HMM-score: 17.7)
    LIDHydrolase; Lipid-droplet associated hydrolase (PF10230; HMM-score: 17.4)
    Abhydrolase_2; Phospholipase/Carboxylesterase (PF02230; HMM-score: 16.5)
    PGAP1; PGAP1-like protein (PF07819; HMM-score: 16.2)
    Peptidase_S9; Prolyl oligopeptidase family (PF00326; HMM-score: 15.9)
    Thioesterase; Thioesterase domain (PF00975; HMM-score: 15.8)
    DUF2920; Protein of unknown function (DUF2920) (PF11144; HMM-score: 15)
    Acyl_transf_2; Acyl transferase (PF02273; HMM-score: 15)
    LCAT; Lecithin:cholesterol acyltransferase (PF02450; HMM-score: 14.8)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.32
    • Cytoplasmic Membrane Score: 9.55
    • Cellwall Score: 0.12
    • Extracellular Score: 0.01
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.104
    • Ymax_pos: 51
    • Cmax: 0.132
    • Cmax_pos: 51
    • Smax: 0.116
    • Smax_pos: 15
    • Smean: 0.083
    • D: 0.096
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MTEKQFKLTVQDNTNIEVKVNFTDVDSKGIIHIFHGMAEHMERYDKLAHALSKHGFDVIRHNHRGHGINIDESTRGHYDDMKRVIGDAFEVAQTVRGNVDKPYIIIGHSMGSVIARLFVETYPQYVDGLILSGTGMYSLWKGLPTVKVLQLITKIYGAEKRVEWVNQLVSNSFNKKIRPLRTQSDWISSNPIEVDNFIKDPYSGFNVSNQLLYQTAYYMLHTSQLKNMKMLNHAMPILLVSGYDDPLGDYGKGILKLANIYRKAGIKNVKVNLYHHKRHEVLFEKDHDKIWEDLFKWLNQFYKK

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]