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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01279
  • pan locus tag?: SAUPAN003615000
  • symbol: SAOUHSC_01279
  • pan gene symbol?:
  • synonym:
  • product: hydrolase alpha/beta fold domain-containing protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_01279
  • symbol: SAOUHSC_01279
  • product: hydrolase alpha/beta fold domain-containing protein
  • replicon: chromosome
  • strand: +
  • coordinates: 1236758..1237672
  • length: 915
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGACTGAAAAACAATTTAAATTAACTGTACAAGATAATACGAATATTGAAGTTAAAGTG
    AATTTTACAGATGTAGATTCAAAAGGAATTATTCATATATTTCATGGTATGGCTGAACAT
    ATGGAACGTTACGATAAATTAGCACATGCACTTTCAAAGCATGGCTTCGATGTGATACGT
    CATAATCATCGAGGACATGGTATTAATATTGATGAATCAACAAGAGGGCATTACGATGAT
    ATGAAACGAGTTATCGGTGATGCCTTTGAAGTAGCGCAAACAGTGAGAGGCAATGTTGAT
    AAACCATACATTATAATCGGACATTCAATGGGATCCGTTATAGCTAGATTGTTTGTAGAA
    ACATATCCGCAATATGTTGATGGTCTAATTTTAAGTGGTACTGGTATGTATTCATTATGG
    AAAGGTTTACCAACCGTTAAAGTGTTACAACTGATTACAAAAATTTATGGTGCTGAGAAA
    CGAGTTGAATGGGTTAACCAGTTAGTATCAAATAGTTTTAATAAAAAAATACGTCCATTA
    CGTACACAAAGTGATTGGATTTCTAGTAATCCAATTGAAGTAGATAACTTTATTAAAGAT
    CCATATAGTGGATTTAATGTGTCAAATCAATTATTATATCAAACAGCCTATTATATGCTA
    CATACATCACAATTAAAAAATATGAAAATGTTAAATCATGCCATGCCTATATTATTAGTT
    TCAGGATATGACGATCCTTTAGGTGATTATGGTAAAGGGATTTTAAAATTGGCGAATATA
    TATAGAAAAGCTGGCATTAAAAATGTTAAAGTGAATCTTTATCATCATAAACGTCATGAA
    GTGTTATTTGAAAAAGATCATGACAAAATTTGGGAAGACTTGTTTAAATGGTTGAATCAA
    TTTTATAAAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    915

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_01279
  • symbol: SAOUHSC_01279
  • description: hydrolase alpha/beta fold domain-containing protein
  • length: 304
  • theoretical pI: 9.65305
  • theoretical MW: 35255.3
  • GRAVY: -0.419408

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersMenaquinone and ubiquinone2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase (TIGR03695; EC 4.2.99.20; HMM-score: 35.4)
    MetabolismEnergy metabolismOther3-oxoadipate enol-lactonase (TIGR02427; EC 3.1.1.24; HMM-score: 25.2)
    2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase (TIGR03343; EC 3.7.1.-; HMM-score: 24.1)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersBiotinpimelyl-[acyl-carrier protein] methyl ester esterase (TIGR01738; EC 3.1.1.85; HMM-score: 23.3)
    proline-specific peptidase (TIGR01250; HMM-score: 22.9)
    MetabolismEnergy metabolismPhotosynthesisputative magnesium chelatase accessory protein (TIGR03056; HMM-score: 14.3)
  • TheSEED:  
    Fatty Acids, Lipids, and IsoprenoidsTriacylglycerolsTriacylglycerol metabolism Lysophospholipase (EC 3.1.1.5) 
    TriacylglycerolsTriacylglycerol metabolism Monoglyceride lipase (EC 3.1.1.23) 
    Triacylglycerol metabolism putative 
  • PFAM:
    AB_hydrolase (CL0028) Hydrolase_4; Serine aminopeptidase, S33 (PF12146; HMM-score: 170.4)
    Abhydrolase_1; alpha/beta hydrolase fold (PF00561; HMM-score: 72.4)
    Abhydrolase_6; Alpha/beta hydrolase family (PF12697; HMM-score: 29.3)
    Abhydrolase_5; Alpha/beta hydrolase family (PF12695; HMM-score: 18.7)
    Peptidase_S28; Serine carboxypeptidase S28 (PF05577; HMM-score: 17.7)
    LIDHydrolase; Lipid-droplet associated hydrolase (PF10230; HMM-score: 17.4)
    Abhydrolase_2; Phospholipase/Carboxylesterase (PF02230; HMM-score: 16.5)
    PGAP1; PGAP1-like protein (PF07819; HMM-score: 16.2)
    Peptidase_S9; Prolyl oligopeptidase family (PF00326; HMM-score: 15.9)
    Thioesterase; Thioesterase domain (PF00975; HMM-score: 15.8)
    DUF2920; Protein of unknown function (DUF2920) (PF11144; HMM-score: 15)
    Acyl_transf_2; Acyl transferase (PF02273; HMM-score: 15)
    LCAT; Lecithin:cholesterol acyltransferase (PF02450; HMM-score: 14.8)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.32
    • Cytoplasmic Membrane Score: 9.55
    • Cellwall Score: 0.12
    • Extracellular Score: 0.01
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.104
    • Ymax_pos: 51
    • Cmax: 0.132
    • Cmax_pos: 51
    • Smax: 0.116
    • Smax_pos: 15
    • Smean: 0.083
    • D: 0.096
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MTEKQFKLTVQDNTNIEVKVNFTDVDSKGIIHIFHGMAEHMERYDKLAHALSKHGFDVIRHNHRGHGINIDESTRGHYDDMKRVIGDAFEVAQTVRGNVDKPYIIIGHSMGSVIARLFVETYPQYVDGLILSGTGMYSLWKGLPTVKVLQLITKIYGAEKRVEWVNQLVSNSFNKKIRPLRTQSDWISSNPIEVDNFIKDPYSGFNVSNQLLYQTAYYMLHTSQLKNMKMLNHAMPILLVSGYDDPLGDYGKGILKLANIYRKAGIKNVKVNLYHHKRHEVLFEKDHDKIWEDLFKWLNQFYKK

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-3661
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]