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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01312
  • pan locus tag?: SAUPAN003688000
  • symbol: SAOUHSC_01312
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_01312
  • symbol: SAOUHSC_01312
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 1256491..1257222
  • length: 732
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGATACTAAGTTACTTGAAAATCGAATTTAAAGTTATAATGCGTAAAAAAACAACATTA
    ATATTATCTATTTTATTTCCTGTTATATTCTATATATTATTTACTTCGATATTGGAATTG
    CCGGAAGATGTTAAACCTAAATTTTATAAAGAGTATATGTATAGTATGACGGTTTATAGT
    TTGTTAAGTTTTAGTTTACTAACTTTTCCATTAGATATTATTAATGAAAAACAAAATGAA
    TGGCGCCAAAGATTAATGGTAACACCATTTACTTTTACTAGTTATTATATTTCAAAAGTA
    GTGAAAACTATGCTGCAATTTGCAATAGCGATATTAGTTATTTTTATGGTTGGACATTTT
    TATAAAGGTGTTGCAATGAGTGCAGTTCAATGGTTAGAGTCAGGAATATTTTTATGGTTA
    GGTGCGTCTCTATTAATAACTTTTGGCATATTATTTTCTTTGTTAAATGATATTCAAAAA
    ACAAGTGCTTTAGCTAATATCGTAACAATTGGTTTAGCAGTATTAGGTGGATTGTGGTTT
    CCGATAAACACATTTCCAAATTGGCTTCAACATGTTGCTCATGTTTTACCGAGCTATCAT
    TTGCGTAAACTAGGTGTAGATATTGCTTCAAATCATCATATCAATTTAATATCATTTGCT
    ATAATACTCTTGTATGCTTTAGGGAGTATAATAGCAGTATATTGTATTAGTCATTTTAAA
    AGGGCGGAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    732

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_01312
  • symbol: SAOUHSC_01312
  • description: hypothetical protein
  • length: 243
  • theoretical pI: 9.7801
  • theoretical MW: 27918.3
  • GRAVY: 0.753498

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    MetabolismTransport and binding proteinsOtherdaunorubicin resistance ABC transporter membrane protein (TIGR01247; HMM-score: 26.3)
    ABC transporter efflux protein, DrrB family (TIGR00025; HMM-score: 25.3)
    UnclassifiedRole category not yet assigneddaunorubicin resistance protein C (TIGR01248; HMM-score: 17.8)
    MetabolismTransport and binding proteinsOtherpigment precursor permease (TIGR00955; HMM-score: 6.3)
    MetabolismTransport and binding proteinsOtherpleiotropic drug resistance family protein (TIGR00956; HMM-score: 2.1)
  • TheSEED:  
    ABC transporter permease protein 
  • PFAM:
    ABC-2 (CL0181) ABC2_membrane_3; ABC-2 family transporter protein (PF12698; HMM-score: 62.7)
    ABC2_membrane; ABC-2 type transporter (PF01061; HMM-score: 50.6)
    ABC2_membrane_4; ABC-2 family transporter protein (PF12730; HMM-score: 20.1)
    ABC2_membrane_2; ABC-2 family transporter protein (PF12679; HMM-score: 19.2)
    no clan definedTctB; Tripartite tricarboxylate transporter TctB family (PF07331; HMM-score: 14)
    IncD; Inclusion membrane protein D (PF17628; HMM-score: 7.3)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 6
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.163
    • Ymax_pos: 16
    • Cmax: 0.141
    • Cmax_pos: 47
    • Smax: 0.324
    • Smax_pos: 7
    • Smean: 0.244
    • D: 0.195
  • predicted transmembrane helices (TMHMM): 6

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MILSYLKIEFKVIMRKKTTLILSILFPVIFYILFTSILELPEDVKPKFYKEYMYSMTVYSLLSFSLLTFPLDIINEKQNEWRQRLMVTPFTFTSYYISKVVKTMLQFAIAILVIFMVGHFYKGVAMSAVQWLESGIFLWLGASLLITFGILFSLLNDIQKTSALANIVTIGLAVLGGLWFPINTFPNWLQHVAHVLPSYHLRKLGVDIASNHHINLISFAIILLYALGSIIAVYCISHFKRAE

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]