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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01372
  • pan locus tag?: SAUPAN003768000
  • symbol: trpA
  • pan gene symbol?: trpA
  • synonym:
  • product: tryptophan synthase subunit alpha

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_01372
  • symbol: trpA
  • product: tryptophan synthase subunit alpha
  • replicon: chromosome
  • strand: +
  • coordinates: 1316026..1316754
  • length: 729
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGACTAAATTATTTATACCTTATATTATGGGCAATAAAGATTTGATTGAAAATGCAACA
    TTGTTGAGTGAAAATGGTGCAGATATAATTGAAATTGGAGTACCTTTCTCTGATCCGGTT
    GCTGATGGTCCAGTTATCATGGAAGCAGGTCAACAAGCGATTAAACAAGGCATCACGATA
    GATTATATTTTCAATCAATTAGAAAAACATGGTGATCAAATTAAGTGTAACTATGTATTA
    ATGACGTATTATAATATTATTTGTCATTATGGAGAACAAGCGTTTTTTGAAAAATGTCGA
    GATACTGGTGTCTACGGCTTAATTATTCCTGATTTACCATATGAATTATCGCAGCGTTTA
    AAACAACAATTTAGTCACTATGGCGTCAAAATCATATCGTTAGTTGCGATGACTACTGAT
    GACAAACGTATAAAAGATATCGTATCCCATGCGGAAGGCTTTATTTATACTGTGACGATG
    AATGCGACAACAGGGCAAAACGGTGCGTTTCATCCAGAATTAAAACGAAAAATTGAGTCA
    ATTAAAGCGATAGCCAATGTGCCAGTTGTCGCAGGATTTGGTATAAGAACACCACAACAT
    GTTGCAGATATAAAAGAGGTTGCAGATGGCATTGTCATTGGTAGCGAAATCGTTAAGCGA
    TTTAAATCTAACACGCGTGAGGAAATCATTAAATATTTACAATCTATCCAACAAACATTG
    AATAATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    729

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_01372
  • symbol: TrpA
  • description: tryptophan synthase subunit alpha
  • length: 242
  • theoretical pI: 6.24515
  • theoretical MW: 27117.1
  • GRAVY: -0.0884297

Function[edit source | edit]

  • reaction:
    EC 4.2.1.20?  ExPASy
    Tryptophan synthaseL-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + D-glyceraldehyde 3-phosphate + H2O
  • TIGRFAM:
    MetabolismAmino acid biosynthesisAromatic amino acid familytryptophan synthase, alpha subunit (TIGR00262; EC 4.2.1.20; HMM-score: 210.2)
    MetabolismAmino acid biosynthesisHistidine familyimidazoleglycerol phosphate synthase, cyclase subunit (TIGR00735; HMM-score: 17.8)
    Genetic information processingProtein fateProtein folding and stabilizationmembrane complex biogenesis protein, BtpA family (TIGR00259; HMM-score: 16)
    geranylgeranylglyceryl phosphate synthase family protein (TIGR01768; EC 2.5.1.-; HMM-score: 13.5)
    Cellular processesCellular processesSporulation and germinationstage II sporulation protein M (TIGR02831; HMM-score: 13.5)
  • TheSEED:  
    Amino Acids and DerivativesAromatic amino acids and derivativesTryptophan synthesis Tryptophan synthase alpha chain (EC 4.2.1.20) 
  • PFAM:
    TIM_barrel (CL0036) Trp_syntA; Tryptophan synthase alpha chain (PF00290; HMM-score: 249)
    BtpA; BtpA family (PF03437; HMM-score: 18.2)
    His_biosynth; Histidine biosynthesis protein (PF00977; HMM-score: 16.3)
    DUF561; Protein of unknown function (DUF561) (PF04481; HMM-score: 16.2)
    IGPS; Indole-3-glycerol phosphate synthase (PF00218; HMM-score: 14.5)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.123
    • Ymax_pos: 19
    • Cmax: 0.11
    • Cmax_pos: 42
    • Smax: 0.203
    • Smax_pos: 11
    • Smean: 0.141
    • D: 0.13
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MTKLFIPYIMGNKDLIENATLLSENGADIIEIGVPFSDPVADGPVIMEAGQQAIKQGITIDYIFNQLEKHGDQIKCNYVLMTYYNIICHYGEQAFFEKCRDTGVYGLIIPDLPYELSQRLKQQFSHYGVKIISLVAMTTDDKRIKDIVSHAEGFIYTVTMNATTGQNGAFHPELKRKIESIKAIANVPVVAGFGIRTPQHVADIKEVADGIVIGSEIVKRFKSNTREEIIKYLQSIQQTLNN

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator: SAOUHSC_01228 (CodY*)
    CodY* (TF) important in Amino acid metabolism

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-3661
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]