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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01369
  • pan locus tag?: SAUPAN003765000
  • symbol: trpC
  • pan gene symbol?: trpC
  • synonym:
  • product: indole-3-glycerol-phosphate synthase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_01369
  • symbol: trpC
  • product: indole-3-glycerol-phosphate synthase
  • replicon: chromosome
  • strand: +
  • coordinates: 1313412..1314194
  • length: 783
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGACGATTTTATCAGAAATTGTTAAATATAAACAGTCACTTTTACAAAATGGCTATTAT
    CAAGACAAACTTAATACCTTGAAAAGTGTGAAGATTCAGAATAAAAAATCTTTTATAAAC
    GCAATTGAGAAAGAACCAAAGCTAGCAATTATTGCAGAAATTAAATCGAAGAGTCCTACA
    GTTAATGACTTACCTGAACGAGATTTATCGCAACAAATCTCAGATTATGACCAATATGGT
    GCAAATGCCGTGTCCATTTTAACTGATGAAAAGTACTTTGGTGGTAGTTTTGAAAGATTA
    CAAGCATTGACGACAAAAACAACATTACCCGTATTATGCAAAGACTTTATTATAGACCCG
    CTTCAAATTGATGTTGCTAAACAAGCTGGTGCATCTATGATTTTATTGATCGTTAACATC
    TTATCTGATAAACAATTGAAAGATTTATATAACTACGCTATATCGCAAAATCTAGAAGTG
    TTAGTTGAAGTACATGATCGCCATGAATTAGAACGTGCCTATAAGGTTAATGCTAAATTG
    ATTGGTGTAAATAACAGGGACTTAAAACGATTTGTTACAAATGTGGAACATACAAATACT
    ATTTTAGAAAATAAAAAAACAAATCATTATTATATTTCTGAAAGTGGTATTCACGATGCA
    TCTGATGTAAGAAAAATCTTGCATAGTGGTATCGATGGCTTACTAATAGGTGAGGCGCTT
    ATGCGTTGTGACAATCTATCTGAATTTTTACCACAACTGAAAATGCAAAAGGTGAAGTCA
    TGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    783

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_01369
  • symbol: TrpC
  • description: indole-3-glycerol-phosphate synthase
  • length: 260
  • theoretical pI: 8.18401
  • theoretical MW: 29526.8
  • GRAVY: -0.310385

Function[edit source | edit]

  • reaction:
    EC 4.1.1.48?  ExPASy
    Indole-3-glycerol-phosphate synthase1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate = 1-C-(3-indolyl)-glycerol 3-phosphate + CO2 + H2O
  • TIGRFAM:
    MetabolismAmino acid biosynthesisHistidine family1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide isomerase (TIGR00007; EC 5.3.1.16; HMM-score: 15)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersPyridine nucleotidesnicotinate-nucleotide diphosphorylase (carboxylating) (TIGR00078; EC 2.4.2.19; HMM-score: 13.9)
  • TheSEED:  
    Amino Acids and DerivativesAromatic amino acids and derivativesTryptophan synthesis Indole-3-glycerol phosphate synthase (EC 4.1.1.48) 
  • PFAM:
    TIM_barrel (CL0036) IGPS; Indole-3-glycerol phosphate synthase (PF00218; HMM-score: 253.3)
    QRPTase_C; Quinolinate phosphoribosyl transferase, C-terminal domain (PF01729; HMM-score: 22)
    FMN_dh; FMN-dependent dehydrogenase (PF01070; HMM-score: 14.6)
    FAD-oxidase_C (CL0277) BBE; Berberine and berberine like (PF08031; HMM-score: 14.4)
    TIM_barrel (CL0036) His_biosynth; Histidine biosynthesis protein (PF00977; HMM-score: 12.4)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.114
    • Ymax_pos: 21
    • Cmax: 0.12
    • Cmax_pos: 42
    • Smax: 0.18
    • Smax_pos: 1
    • Smean: 0.117
    • D: 0.115
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MTILSEIVKYKQSLLQNGYYQDKLNTLKSVKIQNKKSFINAIEKEPKLAIIAEIKSKSPTVNDLPERDLSQQISDYDQYGANAVSILTDEKYFGGSFERLQALTTKTTLPVLCKDFIIDPLQIDVAKQAGASMILLIVNILSDKQLKDLYNYAISQNLEVLVEVHDRHELERAYKVNAKLIGVNNRDLKRFVTNVEHTNTILENKKTNHYYISESGIHDASDVRKILHSGIDGLLIGEALMRCDNLSEFLPQLKMQKVKS

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator: SAOUHSC_01228 (CodY*)
    CodY* (TF) important in Amino acid metabolism

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-3661
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]