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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01386
  • pan locus tag?: SAUPAN003795000
  • symbol: SAOUHSC_01386
  • pan gene symbol?: pstA
  • synonym:
  • product: phosphate ABC transporter permease

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_01386
  • symbol: SAOUHSC_01386
  • product: phosphate ABC transporter permease
  • replicon: chromosome
  • strand: -
  • coordinates: 1329327..1330244
  • length: 918
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGGAAACGACAGATAATAATAGACAATCACTCGTCGATCAACAACTTGTCCAAAAACAT
    TTATCATCCAGAACGGTTAAAAATAAAGTGTTCAAACTCATATTTTTAGCATGTACATTA
    TTAGGACTTGTCGTACTTATTGCGTTGTTAACTCAAACATTGATTAAAGGGGTAAGTCAT
    TTAAATTTACAGTTTTTCACTAATTTTTCTTCTTCAACACCATCTATGGCTGGCGTTAAA
    GGCGCGTTAATCGGTTCACTTTGGTTAATGTTAAGTATCATTCCATTATCAATCATCCTA
    GGAATAGGTACAGCTATATACTTAGAAGAATATGCGAAAAACAACAAATTTACTCAGTTT
    GTTAAAATCAGTATTTCCAATTTAGCTGGTGTACCATCAGTTGTATTTGGGTTATTAGGT
    TATACTTTGTTCGTTGGTGGTGCAGGGATTGAAGCCTTGAAAATGGGTAACAGTATATTG
    GCAGCAGCGCTAACAATGACCTTACTGATATTACCAATTATTATTGTTTCAAGTCAGGAA
    GCAATTAGAGCTGTACCTAACTCAGTACGCGAAGCTTCTTACGGCTTAGGTGCTAATAAA
    TGGCAAACGATAAGACGTGTTGTCTTACCAGCAGCGTTACCTGGTATTTTAACTGGATTC
    ATTTTGTCTCTTTCAAGAGCACTGGGAGAAACAGCGCCACTTGTGCTAATCGGTATACCG
    ACTATATTATTGGCAACACCTAGAAGTATATTGGATCAATTTTCAGCATTACCTATCCAA
    ATATTTACTTGGGCGAAAATGCCTCAAGAAGAATTCCAGAATGTTGCATCGGCAGGCATT
    ATCGTTTTACTAGTTATCTTAATCTTAATGAATGGCGTTGCGATTATTTTACGTAACAAA
    TTTAGTAAAAAATTCTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    918

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_01386
  • symbol: SAOUHSC_01386
  • description: phosphate ABC transporter permease
  • length: 305
  • theoretical pI: 10.7035
  • theoretical MW: 32870.1
  • GRAVY: 0.751803

Function[edit source | edit]

  • TIGRFAM:
    MetabolismTransport and binding proteinsAnionsphosphate ABC transporter, permease protein PstA (TIGR00974; HMM-score: 303.3)
    and 13 more
    MetabolismTransport and binding proteinsAnionsphosphate ABC transporter, permease protein PstC (TIGR02138; HMM-score: 142.3)
    NifC-like ABC-type porter (TIGR01581; HMM-score: 49.9)
    MetabolismTransport and binding proteinsAnionsmolybdate ABC transporter, permease protein (TIGR02141; HMM-score: 48.5)
    MetabolismTransport and binding proteinsAnionssulfate ABC transporter, permease protein CysT (TIGR02139; HMM-score: 48.4)
    MetabolismTransport and binding proteinsAnionssulfate ABC transporter, permease protein (TIGR00969; HMM-score: 45.1)
    2-aminoethylphosphonate ABC transport system, membrane component PhnV (TIGR03255; HMM-score: 40.5)
    2-aminoethylphosphonate ABC transporter, permease protein (TIGR03226; HMM-score: 33.4)
    MetabolismTransport and binding proteinsAnionssulfate ABC transporter, permease protein CysW (TIGR02140; HMM-score: 29.7)
    MetabolismTransport and binding proteinsAmino acids, peptides and aminesputative 2-aminoethylphosphonate ABC transporter, permease protein (TIGR03262; HMM-score: 22.6)
    choline ABC transporter, permease protein (TIGR03416; HMM-score: 19.2)
    ectoine/hydroxyectoine ABC transporter, permease protein EhuC (TIGR03004; HMM-score: 18.9)
    MetabolismTransport and binding proteinsAnionsnitrate ABC transporter, permease protein (TIGR01183; HMM-score: 17.3)
    MetabolismTransport and binding proteinsAnionsphosphonate ABC transporter, permease protein PhnE (TIGR01097; HMM-score: 16.4)
  • TheSEED:  
    Phosphorus MetabolismPhosphorus Metabolism - no subcategoryHigh affinity phosphate transporter and control of PHO regulon Phosphate transport system permease protein PstA (TC 3.A.1.7.1) 
    and 1 more
    Phosphorus Metabolism - no subcategoryPhosphate metabolism Phosphate transport system permease protein PstA (TC 3.A.1.7.1) 
  • PFAM:
    BPD_transp_1 (CL0404) BPD_transp_1; Binding-protein-dependent transport system inner membrane component (PF00528; HMM-score: 88.6)
    and 4 more
    no clan definedDUF3333; Domain of unknown function (DUF3333) (PF11812; HMM-score: 11.6)
    CrgA; Cell division protein CrgA (PF06781; HMM-score: 10.2)
    Piezo_RRas_bdg; Piezo non-specific cation channel, R-Ras-binding domain (PF12166; HMM-score: 9.8)
    DUF3094; Protein of unknown function (DUF3094) (PF11293; HMM-score: 9.2)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 6
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: -0.5
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.17
    • Ymax_pos: 40
    • Cmax: 0.156
    • Cmax_pos: 58
    • Smax: 0.395
    • Smax_pos: 32
    • Smean: 0.175
    • D: 0.172
  • predicted transmembrane helices (TMHMM): 6

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • METTDNNRQSLVDQQLVQKHLSSRTVKNKVFKLIFLACTLLGLVVLIALLTQTLIKGVSHLNLQFFTNFSSSTPSMAGVKGALIGSLWLMLSIIPLSIILGIGTAIYLEEYAKNNKFTQFVKISISNLAGVPSVVFGLLGYTLFVGGAGIEALKMGNSILAAALTMTLLILPIIIVSSQEAIRAVPNSVREASYGLGANKWQTIRRVVLPAALPGILTGFILSLSRALGETAPLVLIGIPTILLATPRSILDQFSALPIQIFTWAKMPQEEFQNVASAGIIVLLVILILMNGVAIILRNKFSKKF

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]