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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01481
  • pan locus tag?: SAUPAN003924000
  • symbol: SAOUHSC_01481
  • pan gene symbol?: aroA
  • synonym:
  • product: 3-phosphoshikimate 1-carboxyvinyltransferase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_01481
  • symbol: SAOUHSC_01481
  • product: 3-phosphoshikimate 1-carboxyvinyltransferase
  • replicon: chromosome
  • strand: -
  • coordinates: 1438012..1439310
  • length: 1299
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGGTAAATGAACAAATCATTGATATTTCAGGTCCGTTAAAGGGCGAAATAGAAGTGCCG
    GGCGATAAGTCAATGACACACCGTGCAATCATGTTGGCGTCGCTAGCTGAAGGTGTATCT
    ACTATATATAAGCCACTACTTGGCGAAGATTGTCGTCGTACGATGGACATTTTCCGACTG
    TTAGGTGTAGAAATCAAAGAAGATGATGAAAAATTAGTTGTGACTTCCCCAGGATATCAA
    TCTTTTAACACGCCACATCAAGTATTGTATACAGGTAATTCTGGTACGACAACACGATTA
    TTGGCAGGTTTGTTAAGTGGTTTAGGTATTGAAAGTGTTTTGTCTGGCGATGTTTCAATT
    GGTAAAAGGCCAATGGATCGTGTCTTGAGACCATTGAAACTTATGGATGCGAATATTGAA
    GGTATTGAAGATAATTATACACCATTAATTATTAAGCCATCTGTCATAAAAGGTATAAAT
    TATCAAATGGAAGTTGCAAGTGCACAAGTAAAAAGTGCCATTTTATTTGCAAGTTTGTTT
    TCTAAGGAACCGACCATCATTAAAGAATTAGATGTAAGTCGAAATCATACTGAGACGATG
    TTCAAACATTTTAATATTCCAATTGAAGCAGAAGGGTTATCAATTAATACAACCCCTGAA
    GCAATTCGATACATTAAACCTGCAGATTTTCATGTTCCTGGCGATATTTCATCTGCAGCG
    TTCTTTATTGTTGCAGCACTTATCACACCAGGAAGTGATGTAACAATTCATAATGTTGGA
    ATCAATCCAACACGTTCAGGTATTATTGATATTGTTGAAAAAATGGGCGGTAATATCCAA
    CTTTTCAATCAAACAACTGGTGCTGAACCTACTGCTTCTATTCGTATTCAATACACACCA
    ATGCTTCAACCAATAACAATCGAAGGAGAATTAGTTCCAAAAGCAATTGATGAACTGCCT
    GTAATAGCATTACTTTGTACACAAGCAGTTGGCACGAGTACAATTAAAGATGCCGAGGAA
    TTAAAAGTAAAAGAAACAAATAGAATTGATACAACGGCTGATATGTTAAACTTGTTAGGG
    TTTGAATTACAACCAACTAATGATGGATTGATTATTCATCCGTCAGAATTTAAAACAAAT
    GCAACAGTTGATAGTTTAACTGATCATCGAATAGGAATGATGCTTGCAGTTGCTTCTCTA
    CTTTCAAGCGAGCCTGTCAAAATCAAACAATTTGATGCTGTAAATGTATCATTTCCAGGA
    TTTTTACCAAAACTAAAGCTTTTAGAAAATGAGGGATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1299

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_01481
  • symbol: SAOUHSC_01481
  • description: 3-phosphoshikimate 1-carboxyvinyltransferase
  • length: 432
  • theoretical pI: 4.77676
  • theoretical MW: 47002.9
  • GRAVY: 0.0409722

Function[edit source | edit]

  • reaction:
    EC 2.5.1.19?  ExPASy
    3-phosphoshikimate 1-carboxyvinyltransferasePhosphoenolpyruvate + 3-phosphoshikimate = phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate
  • TIGRFAM:
    MetabolismAmino acid biosynthesisAromatic amino acid family3-phosphoshikimate 1-carboxyvinyltransferase (TIGR01356; EC 2.5.1.19; HMM-score: 379.1)
    Cell structureCell envelopeBiosynthesis and degradation of murein sacculus and peptidoglycanUDP-N-acetylglucosamine 1-carboxyvinyltransferase (TIGR01072; EC 2.5.1.7; HMM-score: 72.7)
  • TheSEED:  
    Chorismate Synthesis 5-Enolpyruvylshikimate-3-phosphate synthase (EC 2.5.1.19) alpha sub-homology division 
    Common Pathway For Synthesis of Aromatic Compounds (DAHP synthase to chorismate) 5-Enolpyruvylshikimate-3-phosphate synthase (EC 2.5.1.19) alpha sub-homology division 
  • PFAM:
    EPT_RTPC (CL0290) EPSP_synthase; EPSP synthase (3-phosphoshikimate 1-carboxyvinyltransferase) (PF00275; HMM-score: 455.3)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.098
    • Ymax_pos: 70
    • Cmax: 0.113
    • Cmax_pos: 39
    • Smax: 0.117
    • Smax_pos: 70
    • Smean: 0.073
    • D: 0.088
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MVNEQIIDISGPLKGEIEVPGDKSMTHRAIMLASLAEGVSTIYKPLLGEDCRRTMDIFRLLGVEIKEDDEKLVVTSPGYQSFNTPHQVLYTGNSGTTTRLLAGLLSGLGIESVLSGDVSIGKRPMDRVLRPLKLMDANIEGIEDNYTPLIIKPSVIKGINYQMEVASAQVKSAILFASLFSKEPTIIKELDVSRNHTETMFKHFNIPIEAEGLSINTTPEAIRYIKPADFHVPGDISSAAFFIVAALITPGSDVTIHNVGINPTRSGIIDIVEKMGGNIQLFNQTTGAEPTASIRIQYTPMLQPITIEGELVPKAIDELPVIALLCTQAVGTSTIKDAEELKVKETNRIDTTADMLNLLGFELQPTNDGLIIHPSEFKTNATVDSLTDHRIGMMLAVASLLSSEPVKIKQFDAVNVSFPGFLPKLKLLENEG

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator: SAOUHSC_01228 (CodY*)
    CodY* (TF) important in Amino acid metabolism

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-3661
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]

C O'Connell, P A Pattee, T J Foster
Sequence and mapping of the aroA gene of Staphylococcus aureus 8325-4.
J. Gen. Microbiol.: 1993, 139(7);1449-60
[PubMed:8371108] [WorldCat.org] [DOI] (P p)