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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01496
  • pan locus tag?: SAUPAN003942000
  • symbol: cmk
  • pan gene symbol?: cmk
  • synonym:
  • product: cytidylate kinase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_01496
  • symbol: cmk
  • product: cytidylate kinase
  • replicon: chromosome
  • strand: -
  • coordinates: 1450481..1451140
  • length: 660
  • essential: yes [1] DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGAAAGCCATTAATATTGCATTAGATGGTCCAGCTGCTGCCGGAAAAAGTACAATTGCG
    AAACGTGTAGCCAGCGAACTATCAATGATTTATGTCGATACAGGAGCAATGTATCGTGCA
    TTAACATACAAATATTTAAAATTAAACAAAACTGAGGACTTTGCAAAACTAGTTGACCAA
    ACAACATTAGATTTAACTTATAAAGCAGATAAAGGTCAATGTGTCATTTTAGATAACGAA
    GATGTAACAGACTTTTTAAGAAATAATGATGTGACGCAACATGTTTCATACGTTGCATCT
    AAAGAGCCAGTACGTTCATTCGCCGTTAAAAAACAAAAAGAGTTAGCTGCAGAAAAAGGT
    ATCGTAATGGATGGTCGCGATATCGGAACTGTAGTGCTACCAGATGCAGATTTAAAAGTA
    TATATGATTGCATCAGTTGAAGAGCGAGCAGAAAGAAGATATAAAGATAATCAATTAAGA
    GGTATCGAATCAAATTTTGAAGATTTAAAACGTGATATTGAAGCTCGTGATCAATATGAC
    ATGAACCGTGAAATATCACCATTAAGAAAAGCAGATGATGCAGTGACATTAGATACGACA
    GGCAAGTCGATTGAAGAAGTTACTGACGAAATTTTAGCGATGGTGAGTCAAATTAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_01496
  • symbol: Cmk
  • description: cytidylate kinase
  • length: 219
  • theoretical pI: 4.8264
  • theoretical MW: 24594.8
  • GRAVY: -0.435616

Function[edit source | edit]

  • reaction:
    EC 2.7.4.25?  ExPASy
    (d)CMP kinaseATP + (d)CMP = ADP + (d)CDP
    EC 2.7.4.14?  ExPASy
    UMP/CMP kinaseATP + (d)CMP = ADP + (d)CDPATP + UMP = ADP + UDP
  • TIGRFAM:
    MetabolismPurines, pyrimidines, nucleosides, and nucleotidesNucleotide and nucleoside interconversionscytidylate kinase (TIGR00017; EC 2.7.4.14; HMM-score: 243.5)
    putative cytidylate kinase (TIGR02173; EC 2.7.4.14; HMM-score: 42.1)
    MetabolismPurines, pyrimidines, nucleosides, and nucleotidesNucleotide and nucleoside interconversionsadenylate kinase (TIGR01351; EC 2.7.4.-; HMM-score: 21.3)
    carbohydrate kinase, thermoresistant glucokinase family (TIGR01313; EC 2.7.1.-; HMM-score: 13.7)
  • TheSEED:  
    Nucleosides and NucleotidesPyrimidinespyrimidine conversions Cytidylate kinase (EC 2.7.4.25) 
  • PFAM:
    P-loop_NTPase (CL0023) Cytidylate_kin; Cytidylate kinase (PF02224; HMM-score: 236.7)
    Cytidylate_kin2; Cytidylate kinase-like family (PF13189; HMM-score: 37.4)
    AAA_18; AAA domain (PF13238; HMM-score: 30.5)
    AAA_33; AAA domain (PF13671; HMM-score: 27.1)
    SKI; Shikimate kinase (PF01202; HMM-score: 23.3)
    AAA_17; AAA domain (PF13207; HMM-score: 19.1)
    AAA_14; AAA domain (PF13173; HMM-score: 15.8)
    AAA_24; AAA domain (PF13479; HMM-score: 14.7)
    ADK; Adenylate kinase (PF00406; HMM-score: 14.7)
    AAA; ATPase family associated with various cellular activities (AAA) (PF00004; HMM-score: 13.7)
    no clan definedTPX2; Targeting protein for Xklp2 (TPX2) (PF06886; HMM-score: 13)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.157
    • Ymax_pos: 15
    • Cmax: 0.112
    • Cmax_pos: 37
    • Smax: 0.319
    • Smax_pos: 11
    • Smean: 0.226
    • D: 0.184
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MKAINIALDGPAAAGKSTIAKRVASELSMIYVDTGAMYRALTYKYLKLNKTEDFAKLVDQTTLDLTYKADKGQCVILDNEDVTDFLRNNDVTQHVSYVASKEPVRSFAVKKQKELAAEKGIVMDGRDIGTVVLPDADLKVYMIASVEERAERRYKDNQLRGIESNFEDLKRDIEARDQYDMNREISPLRKADDAVTLDTTGKSIEEVTDEILAMVSQIK

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Roy R Chaudhuri, Andrew G Allen, Paul J Owen, Gil Shalom, Karl Stone, Marcus Harrison, Timothy A Burgis, Michael Lockyer, Jorge Garcia-Lara, Simon J Foster, Stephen J Pleasance, Sarah E Peters, Duncan J Maskell, Ian G Charles
    Comprehensive identification of essential Staphylococcus aureus genes using Transposon-Mediated Differential Hybridisation (TMDH).
    BMC Genomics: 2009, 10;291
    [PubMed:19570206] [WorldCat.org] [DOI] (I e)
  2. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-3661
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]