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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01602
  • pan locus tag?: SAUPAN004044000
  • symbol: SAOUHSC_01602
  • pan gene symbol?: malR
  • synonym:
  • product: maltose operon transcriptional repressor

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_01602
  • symbol: SAOUHSC_01602
  • product: maltose operon transcriptional repressor
  • replicon: chromosome
  • strand: -
  • coordinates: 1527876..1528895
  • length: 1020
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGGTTACGATTAAAGATGTTGCACTAAAAGCCGGTGTTTCTCCTTCGACAGTTTCAAGA
    GTTATAAAAGGAAATCAACGTATTAGCGAAGCGACAATTTCAAAAGTGAAGAAAGTTATG
    GAAGAATTGAATTATTTTCCTAATACCGCTGCTAGAACTTTAATTACAAACCAAACATAT
    AAAATTGGTTTAGTGTTAAAAGGGTCTGAGGAGCCTATTCGACTGAATCCATTCTACATC
    AATGTATTGCTAGGAATTTCTGAAACGTGTAACCAGCATGGCTATGGTACACAAACGACA
    GTCTCAAATAATATGAATGATTTAATGGATGAAGTTTATAAAATGATTAAACAACGAATG
    GTTGATGCGTTTATACTGCTCTATTCAAAAGAAAATGATCCGATTAAACAAATGTTAATT
    GATGAAAGCATGCCATTTATTGTGATTGGTAAGCCTACATCGGATATAGATCATCAATTT
    ACACATATAGATAATGATAATATATTAGCTTCTGAAAATTTGACACGACATGTTATTGAA
    CAAGGTGTAGATGAATTAATATTTATTACAGAAAAAGGAAATTTTGAAGTTTCAAAAGAT
    AGAATTCAAGGATTTGAAACGATAGCTTCTCAAAATAAAATTAATTATCAAATTATTGAG
    ACTAGTAATGAAAGAGAAGTTATTTTTAATTACATGCAAAATCTACATACGCGTTTGAAG
    GACCCAAATATTAAACAGGCAATCATTTCGTTAGATGCTATGTTACATTTAGCGATTTTA
    AGTGTCCTATATGAACTTAATATTGAAATTCCGAAAGATGTAATGACAGCAACGTTCAAT
    GATTCTTATTTAACCGAAATTGCGTCACCACCACAAACCTGTATTGACATCAAGCCTCGA
    ATGTTAGGTCAACAGGCTGGTGCAGCAATTTTAAATATATTGAAAAACAAAGCACAGGAT
    GTTATTGAACTCGTCATTATAGATACAGAATTAAAAATAAGAAAATCAACACAGCGATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_01602
  • symbol: SAOUHSC_01602
  • description: maltose operon transcriptional repressor
  • length: 339
  • theoretical pI: 5.7117
  • theoretical MW: 38390.1
  • GRAVY: -0.179056

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Signal transductionRegulatory functionsDNA interactionscatabolite control protein A (TIGR01481; HMM-score: 117.9)
    Signal transductionRegulatory functionsDNA interactionsD-fructose-responsive transcription factor (TIGR02417; HMM-score: 111.6)
    Signal transductionRegulatory functionsDNA interactionstrehalose operon repressor (TIGR02405; HMM-score: 50.9)
    Genetic information processingMobile and extrachromosomal element functionsOtheraddiction module antidote protein, HigA family (TIGR02607; HMM-score: 16.2)
    Signal transductionRegulatory functionsDNA interactionsaddiction module antidote protein, HigA family (TIGR02607; HMM-score: 16.2)
    Signal transductionRegulatory functionsProtein interactionsaddiction module antidote protein, HigA family (TIGR02607; HMM-score: 16.2)
    Hypothetical proteinsConservedTIGR02147 family protein (TIGR02147; HMM-score: 14.8)
  • TheSEED:  
    CarbohydratesDi- and oligosaccharidesMaltose and Maltodextrin Utilization Maltose operon transcriptional repressor MalR, LacI family 
  • PFAM:
    HTH (CL0123) LacI; Bacterial regulatory proteins, lacI family (PF00356; HMM-score: 75.8)
    Periplas_BP (CL0144) Peripla_BP_3; Periplasmic binding protein-like domain (PF13377; HMM-score: 68.4)
    Peripla_BP_1; Periplasmic binding proteins and sugar binding domain of LacI family (PF00532; HMM-score: 61.3)
    HTH (CL0123) HTH_3; Helix-turn-helix (PF01381; HMM-score: 27.5)
    HTH_38; Helix-turn-helix domain (PF13936; HMM-score: 17.5)
    HTH_Tnp_ISL3; Helix-turn-helix domain of transposase family ISL3 (PF13542; HMM-score: 16.4)
    HTH_31; Helix-turn-helix domain (PF13560; HMM-score: 15.3)
    YdaS_antitoxin; Putative antitoxin of bacterial toxin-antitoxin system, YdaS/YdaT (PF15943; HMM-score: 14.9)
    HTH_AraC; Bacterial regulatory helix-turn-helix proteins, AraC family (PF00165; HMM-score: 13.7)
    HTH_19; Helix-turn-helix domain (PF12844; HMM-score: 13.4)
    HTH_24; Winged helix-turn-helix DNA-binding (PF13412; HMM-score: 12.5)
    HTH_23; Homeodomain-like domain (PF13384; HMM-score: 12.5)
    HTH_28; Helix-turn-helix domain (PF13518; HMM-score: 12.5)
    HTH_17; Helix-turn-helix domain (PF12728; HMM-score: 12.5)
    no clan definedDUF5351; Family of unknown function (DUF5351) (PF17302; HMM-score: 11.9)
    HTH (CL0123) MerR; MerR family regulatory protein (PF00376; HMM-score: 11.7)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.209
    • Ymax_pos: 11
    • Cmax: 0.149
    • Cmax_pos: 21
    • Smax: 0.511
    • Smax_pos: 1
    • Smean: 0.403
    • D: 0.285
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MVTIKDVALKAGVSPSTVSRVIKGNQRISEATISKVKKVMEELNYFPNTAARTLITNQTYKIGLVLKGSEEPIRLNPFYINVLLGISETCNQHGYGTQTTVSNNMNDLMDEVYKMIKQRMVDAFILLYSKENDPIKQMLIDESMPFIVIGKPTSDIDHQFTHIDNDNILASENLTRHVIEQGVDELIFITEKGNFEVSKDRIQGFETIASQNKINYQIIETSNEREVIFNYMQNLHTRLKDPNIKQAIISLDAMLHLAILSVLYELNIEIPKDVMTATFNDSYLTEIASPPQTCIDIKPRMLGQQAGAAILNILKNKAQDVIELVIIDTELKIRKSTQR

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-3661
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]