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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01635
  • pan locus tag?: SAUPAN004116000
  • symbol: SAOUHSC_01635
  • pan gene symbol?: aroK
  • synonym:
  • product: shikimate kinase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_01635
  • symbol: SAOUHSC_01635
  • product: shikimate kinase
  • replicon: chromosome
  • strand: -
  • coordinates: 1556067..1556591
  • length: 525
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    ATGAATCATGATAAATCACCAATAATTTTAATTGGTTTCATGGGTACCGGTAAATCTACG
    ATTGGTAAATACGTTGCAGATGAGCAAAATTTATCATTTATTGATATAGATTCATATATC
    GAAGAGAAGTATAAGTTAACAATACCAGAAATATTTTGTAAACATGGTGAACAATATTTC
    AGGAATTTAGAGTTCACATGTTTGCAAGAATGTATTAACACTGCAGATATAATTGCTACT
    GGTGGTGGTATTATTGAGAGTGAAGAGGCATTTAATTTTTTGAAAAATCAAAAAAACATT
    ATTTGGTTAGATTGTAATATTGATATTATATATAGTCGAATCAATGATGACCCACATCGA
    CCTAATGCAAATAATAAGACAATCAAGCAGTTAAATGACTTGTATTGCTCGCGGAATTTA
    AGATATAATGAAATCGCATTCAAGAAATTTGATAGTCATTTGCTATCAATTTCAGAAATA
    TATTATGAATTGCTAAATTTAATAAAAGCGAGTGATCAGTATTAG
    60
    120
    180
    240
    300
    360
    420
    480
    525

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_01635
  • symbol: SAOUHSC_01635
  • description: shikimate kinase
  • length: 174
  • theoretical pI: 4.87098
  • theoretical MW: 20221.8
  • GRAVY: -0.344253

Function[edit source | edit]

  • reaction:
    EC 2.7.1.71?  ExPASy
    Shikimate kinaseATP + shikimate = ADP + shikimate 3-phosphate
  • TIGRFAM:
    carbohydrate kinase, thermoresistant glucokinase family (TIGR01313; EC 2.7.1.-; HMM-score: 19.5)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersPantothenate and coenzyme Adephospho-CoA kinase (TIGR00152; EC 2.7.1.24; HMM-score: 19.3)
    putative cytidylate kinase (TIGR02173; EC 2.7.4.14; HMM-score: 17.6)
  • TheSEED:  
    Amino Acids and DerivativesAromatic amino acids and derivativesChorismate Synthesis Shikimate kinase I (EC 2.7.1.71) 
    Aromatic amino acids and derivativesCommon Pathway For Synthesis of Aromatic Compounds (DAHP synthase to chorismate) Shikimate kinase I (EC 2.7.1.71) 
    Regulation and Cell signalingRegulation and Cell signaling - no subcategoryCell envelope-associated LytR-CpsA-Psr transcriptional attenuators Shikimate kinase I (EC 2.7.1.71) 
  • PFAM:
    P-loop_NTPase (CL0023) SKI; Shikimate kinase (PF01202; HMM-score: 146.2)
    AAA_18; AAA domain (PF13238; HMM-score: 32.3)
    AAA; ATPase family associated with various cellular activities (AAA) (PF00004; HMM-score: 20.2)
    AAA_33; AAA domain (PF13671; HMM-score: 15.6)
    AAA_22; AAA domain (PF13401; HMM-score: 15.6)
    Thioredoxin (CL0172) DUF836; Glutaredoxin-like domain (DUF836) (PF05768; HMM-score: 13.5)
    P-loop_NTPase (CL0023) AAA_24; AAA domain (PF13479; HMM-score: 11.8)
    CoaE; Dephospho-CoA kinase (PF01121; HMM-score: 11.5)
    Cytidylate_kin; Cytidylate kinase (PF02224; HMM-score: 11.3)

Structure, modifications & interactions[edit source | edit]

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.185
    • Ymax_pos: 27
    • Cmax: 0.189
    • Cmax_pos: 27
    • Smax: 0.277
    • Smax_pos: 22
    • Smean: 0.158
    • D: 0.174
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MNHDKSPIILIGFMGTGKSTIGKYVADEQNLSFIDIDSYIEEKYKLTIPEIFCKHGEQYFRNLEFTCLQECINTADIIATGGGIIESEEAFNFLKNQKNIIWLDCNIDIIYSRINDDPHRPNANNKTIKQLNDLYCSRNLRYNEIAFKKFDSHLLSISEIYYELLNLIKASDQY

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)
  2. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-3661
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]