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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01633
  • pan locus tag?: SAUPAN004113000
  • symbol: SAOUHSC_01633
  • pan gene symbol?: gcvPA
  • synonym:
  • product: glycine dehydrogenase subunit 1

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_01633
  • symbol: SAOUHSC_01633
  • product: glycine dehydrogenase subunit 1
  • replicon: chromosome
  • strand: -
  • coordinates: 1553451..1554797
  • length: 1347
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    GTGAGTCATCGTTATATACCTTTAACTGAAAAAGACAAGCAAGAAATGTTACAAACAATT
    GGTGCAAAATCTATAGGAGAATTATTCGGTGATGTACCAAGTGACATTTTATTAAATAGA
    GATTTAAATATTGCTGAAGGCGAAGCGGAAACAACGTTACTTAGAAGATTAAATCGCATT
    GCAAGCAAGAATATCACTAAAGAAACGCATACATCATTTTTGGGTGCTGGTGTTTATGAT
    CATTACGCGCCATCAGTTGTGGATGCTATGATTTCAAGATCAGAATTTTACACAGCATAT
    ACGCCATATCAACCGGAAATTTCTCAAGGTGAATTACAAGCCATTTTTGAGTTCCAAACT
    TTAATTTGTGAATTAACAGATATGGATGTAGCAAATTCTTCTATGTATGATGGGATGACT
    AGCTTTGCTGAAGCATGTATCTTAGCATTTAGTCAAACAAAGAAAAATAAAATTGTAGTC
    TCAAAAGGTTTACATTATCAAGCATTACAAGTACTTCATACTTATGCTAAGACGCGTAAG
    GAATTCGAAGTAGTGGAAATCGATTTAGATGGCACTGTTACAGATTTGAAAAAATTAGAA
    GCAGCAGTGGATGATGAAACAGCAGCTGTTGCAGTACAATATCCAAACTTTTACGGTTCA
    ATTGAAGATCTTGAAAAGATTCATAGCTTTATTGAAGATAAAAAAGCATTATTTATCGTA
    TATGCAAATCCATTAGCATTGGGATTACTTACACCTCCAGGTTCATTTGGTGCAGATATT
    GTTGTAGGTGATACTCAGCCATTCGGAATTCCTGCTCAATTCGGTGGGCCACATTGTGGA
    TATTTTGCAACGACGAAAAAGTTAATGCGTAAAGTGCCAGGTAGATTAGTCGGTCAAACA
    CAAGATGATGAAGGTAATCGTGGCTTTGTATTAACATTACAAGCACGTGAACAACATATT
    CGACGCGATAAAGCGACATCTAATATTTGTTCTAACCAAGCATTAAATGCTTTAGCGTCA
    TCAATTGCAATGTCAGCACTTGGTAAACAAGGTATCTATGATATTGCAGTTCAAAATATA
    GAACATGCTAACTATGCTAAACAACAATTTATTAAAAAAGGTTTTGAAGTATTAGATGGT
    ACATCTTTCAATGAGTTTGTAGTCAAATTTGACAAACCAATTCAACAAGTTAACGAGGAA
    TTAGTGAAGTACAATATTATCGGTGGTTTTGATTTAGGTGTTGTTTCAGACGACTTTAAA
    AATCACATGTTAATAGCTGTAACTGAATTAAGAACAAAAGATGAAATCGATACATTTGTA
    GAAAAGGCTGGTGAGTTAAATGACTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1347

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_01633
  • symbol: SAOUHSC_01633
  • description: glycine dehydrogenase subunit 1
  • length: 448
  • theoretical pI: 4.83131
  • theoretical MW: 49716.1
  • GRAVY: -0.201339

Function[edit source | edit]

  • reaction:
    EC 1.4.4.2?  ExPASy
    Glycine dehydrogenase (aminomethyl-transferring)Glycine + [glycine-cleavage complex H protein]-N6-lipoyl-L-lysine = [glycine-cleavage complex H protein]-S-aminomethyl-N6-dihydrolipoyl-L-lysine + CO2
  • TIGRFAM:
    MetabolismEnergy metabolismAmino acids and aminesglycine dehydrogenase (TIGR00461; EC 1.4.4.2; HMM-score: 316.7)
    cysteine desulfurase NifS (TIGR03402; EC 2.8.1.7; HMM-score: 27.3)
    Genetic information processingProtein synthesistRNA and rRNA base modificationcysteine desulfurase IscS (TIGR02006; EC 2.8.1.7; HMM-score: 25.2)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersOthercysteine desulfurase IscS (TIGR02006; EC 2.8.1.7; HMM-score: 25.2)
    Unknown functionEnzymes of unknown specificitycysteine desulfurase family protein (TIGR01977; HMM-score: 25)
    Genetic information processingDNA metabolismRestriction/modificationcysteine desulfurase DndA (TIGR03235; EC 2.8.1.7; HMM-score: 19.9)
    MetabolismEnergy metabolismAmino acids and aminestyrosine aminotransferase (TIGR01264; EC 2.6.1.5; HMM-score: 14.6)
    tyrosine/nicotianamine family aminotransferase (TIGR01265; HMM-score: 12)
    cysteine desulfurase, NifS family (TIGR03403; EC 2.8.1.7; HMM-score: 11.9)
  • TheSEED:  
    Amino Acids and DerivativesAlanine, serine, and glycineGlycine and Serine Utilization Glycine dehydrogenase [decarboxylating] (glycine cleavage system P1 protein) (EC 1.4.4.2) 
    Alanine, serine, and glycineGlycine cleavage system Glycine dehydrogenase [decarboxylating] (glycine cleavage system P1 protein) (EC 1.4.4.2) 
  • PFAM:
    PLP_aminotran (CL0061) GDC-P; Glycine cleavage system P-protein (PF02347; HMM-score: 506.5)
    Aminotran_5; Aminotransferase class-V (PF00266; HMM-score: 22.8)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.123
    • Ymax_pos: 23
    • Cmax: 0.128
    • Cmax_pos: 23
    • Smax: 0.15
    • Smax_pos: 21
    • Smean: 0.112
    • D: 0.119
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MSHRYIPLTEKDKQEMLQTIGAKSIGELFGDVPSDILLNRDLNIAEGEAETTLLRRLNRIASKNITKETHTSFLGAGVYDHYAPSVVDAMISRSEFYTAYTPYQPEISQGELQAIFEFQTLICELTDMDVANSSMYDGMTSFAEACILAFSQTKKNKIVVSKGLHYQALQVLHTYAKTRKEFEVVEIDLDGTVTDLKKLEAAVDDETAAVAVQYPNFYGSIEDLEKIHSFIEDKKALFIVYANPLALGLLTPPGSFGADIVVGDTQPFGIPAQFGGPHCGYFATTKKLMRKVPGRLVGQTQDDEGNRGFVLTLQAREQHIRRDKATSNICSNQALNALASSIAMSALGKQGIYDIAVQNIEHANYAKQQFIKKGFEVLDGTSFNEFVVKFDKPIQQVNEELVKYNIIGGFDLGVVSDDFKNHMLIAVTELRTKDEIDTFVEKAGELND

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-3661
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]