NCBI: 03-AUG-2016
Contents
⊟Summary[edit source | edit]
- organism: Staphylococcus aureus NCTC8325
- locus tag: SAOUHSC_01663
- pan locus tag?: SAUPAN004143000
- symbol: SAOUHSC_01663
- pan gene symbol?: dnaG
- synonym:
- product: DNA primase
⊟Genome View[edit source | edit]
⊟Gene[edit source | edit]
⊟General[edit source | edit]
- type: CDS
- locus tag: SAOUHSC_01663
- symbol: SAOUHSC_01663
- product: DNA primase
- replicon: chromosome
- strand: -
- coordinates: 1575828..1577627
- length: 1800
- essential: yes [1] DEG other strains
⊟Accession numbers[edit source | edit]
- Gene ID: 3920075 NCBI
- RefSeq: YP_500174 NCBI
- MicrobesOnline: 1290088 MicrobesOnline
⊟Phenotype[edit source | edit]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit source | edit]
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1741TTGCGAATAGATCAATCGATCATTAATGAAATAAAAGATAAAACCGACATTTTAGACTTG
GTAAGTGAATATGTAAAATTAGAAAAGAGAGGACGCAATTATATAGGTTTGTGTCCTTTT
CATGATGAAAAGACACCTTCATTTACAGTTTCTGAAGATAAACAAATTTGTCATTGTTTT
GGTTGTAAAAAAGGTGGCAATGTTTTTCAATTTACTCAAGAAATTAAAGACATATCATTT
GTTGAAGCGGTTAAAGAATTAGGTGATAGAGTTAATGTTGCTGTAGATATTGAGGCAACA
CAATCTAACTCAAATGTTCAAATTGCTTCTGATGATTTACAAATGATTGAAATGCATGAG
TTAATACAAGAATTTTATTATTACGCTTTAACAAAGACAGTCGAAGGCGAACAAGCATTA
ACGTACTTACAAGAACGTGGTTTTACAGATGCGCTTATTAAAGAGCGAGGCATTGGCTTT
GCACCCGATAGCTCACATTTTTGTCATGATTTTCTTCAAAAAAAGGGTTACGATATTGAA
TTAGCATATGAAGCCGGATTATTATCACGTAACGAAGAAAATTTCAGTTATTACGATAGA
TTTCGAAATCGTATTATGTTTCCTTTGAAAAATGCGCAAGGAAGAATTGTTGGATATTCA
GGTCGAACATATACCGGTCAAGAACCAAAATACTTAAATAGTCCTGAAACACCTATCTTT
CAAAAAAGAAAGTTGTTATACAACTTAGATAAAGCGCGTAAATCAATTAGAAAATTAGAT
GAAATCGTATTACTAGAAGGTTTTATGGATGTTATAAAATCTGATACTGCTGGCTTGAAA
AACGTTGTTGCAACAATGGGTACACAGTTGTCAGATGAACATATTACTTTTATACGAAAG
TTAACATCAAATATAACATTAATGTTTGATGGGGATTTTGCGGGTAGTGAAGCAACACTT
AAAACAGGTCAAAATTTGTTACAGCAAGGGCTAAATGTATTTGTTATACAATTGCCATCA
GGCATGGATCCGGATGAATACATTGGTAAGTATGGCAACGATGCATTTACTGCTTTTGTA
AAAAATGACAAAAAGTCATTTGCACATTATAAAGTGAGTATATTAAAAGATGAAATTGCA
CATAATGACCTTTCATATGAACGTTATTTGAAAGAACTAAGTCATGATATTTCGCTTATG
AAATCATCGATTTTGCAACAAAAGGCTTTAAATGATGTTGCACCATTTTTCAATGTTAGT
CCTGAGCAATTAGCTAACGAAATACAATTCAATCAAGCACCAGCCAATTATTATCCAGAA
GATGAGTATGGCGGTTACATTGAACCTGAGCCAATTGGTATGGCACAATTTGACAATTTG
AGCCGTCAAGAAAAAGCGGAGCGAGCATTTTTAAAACATTTAATGAGAGATAAAGATACA
TTTTTAAATTATTATGAAAGTGTTGATAAGGATAACTTCACAAATCAGCATTTTAAATAT
GTATTCGAAGTCTTACATGATTTTTATGCGGAAAATGATCAATATAATATCAGTGATGCT
GTGCAGTATGTTAATTCAAATGAGTTGAGAGAAACACTAATTAGCTTAGAACAATATAAT
TTGAATGACGAACCATATGAAAATGAAATTGATGATTATGTCAATGTTATTAATGAAAAA
GGACAAGAAACAATTGAGTCATTGAATCATAAATTAAGGGAAGCTACAAGGATTGGCGAT
GTAGAATTACAAAAATACTATTTACAGCAAATTGTTGCTAAGAATAAAGAACGCATGTAG60
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⊟Protein[edit source | edit]
⊟General[edit source | edit]
- locus tag: SAOUHSC_01663
- symbol: SAOUHSC_01663
- description: DNA primase
- length: 599
- theoretical pI: 4.71665
- theoretical MW: 69293.3
- GRAVY: -0.571619
⊟Function[edit source | edit]
- reaction: EC 2.7.7.-? ExPASy
- TIGRFAM: DNA metabolismDNA replication, recombination, and repairDNA primase (TIGR01391; EC 2.7.7.-; HMM-score: 509.6)Unknown functionGeneralDNA primase-like protein (TIGR00646; HMM-score: 33.6)DNA metabolismDNA replication, recombination, and repairreplicative DNA helicase (TIGR00665; EC 3.6.4.12; HMM-score: 21.1)TranscriptionRNA processingribonuclease M5 (TIGR00334; EC 3.1.26.8; HMM-score: 16.7)Mobile and extrachromosomal element functionsProphage functionsphage replicative helicase, DnaB family (TIGR03600; HMM-score: 14.2)
- TheSEED:
- PFAM: no clan definedToprim_N; DNA primase catalytic core, N-terminal domain (PF08275; HMM-score: 139.3)Zn_Beta_Ribbon (CL0167) zf-CHC2; CHC2 zinc finger (PF01807; HMM-score: 130)Toprim-like (CL0413) Toprim_4; Toprim domain (PF13662; HMM-score: 53.5)Toprim_2; Toprim-like (PF13155; HMM-score: 52.9)no clan definedDnaB; DnaB-like helicase N terminal domain (PF00772; HMM-score: 26.6)Toprim-like (CL0413) Toprim_3; Toprim domain (PF13362; HMM-score: 26.5)Toprim; Toprim domain (PF01751; HMM-score: 16.8)
⊟Structure, modifications & interactions[edit source | edit]
- domains:
- modifications:
- cofactors: Mg2+, Zn2+
- effectors:
- protein partners:
⊟Localization[edit source | edit]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helices: 0
- LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular Possibility: 1
- Signal Peptide Possibility: -1
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- Ymax: 0.109
- Ymax_pos: 60
- Cmax: 0.107
- Cmax_pos: 60
- Smax: 0.148
- Smax_pos: 47
- Smean: 0.083
- D: 0.099
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit source | edit]
⊟Protein sequence[edit source | edit]
- MRIDQSIINEIKDKTDILDLVSEYVKLEKRGRNYIGLCPFHDEKTPSFTVSEDKQICHCFGCKKGGNVFQFTQEIKDISFVEAVKELGDRVNVAVDIEATQSNSNVQIASDDLQMIEMHELIQEFYYYALTKTVEGEQALTYLQERGFTDALIKERGIGFAPDSSHFCHDFLQKKGYDIELAYEAGLLSRNEENFSYYDRFRNRIMFPLKNAQGRIVGYSGRTYTGQEPKYLNSPETPIFQKRKLLYNLDKARKSIRKLDEIVLLEGFMDVIKSDTAGLKNVVATMGTQLSDEHITFIRKLTSNITLMFDGDFAGSEATLKTGQNLLQQGLNVFVIQLPSGMDPDEYIGKYGNDAFTAFVKNDKKSFAHYKVSILKDEIAHNDLSYERYLKELSHDISLMKSSILQQKALNDVAPFFNVSPEQLANEIQFNQAPANYYPEDEYGGYIEPEPIGMAQFDNLSRQEKAERAFLKHLMRDKDTFLNYYESVDKDNFTNQHFKYVFEVLHDFYAENDQYNISDAVQYVNSNELRETLISLEQYNLNDEPYENEIDDYVNVINEKGQETIESLNHKLREATRIGDVELQKYYLQQIVAKNKERM
⊟Experimental data[edit source | edit]
- experimentally validated: PeptideAtlas [2] [3]
⊟Expression & Regulation[edit source | edit]
⊟Operon[edit source | edit]
- MicrobesOnline: SAOUHSC_01663 no polycistronic organisation predicted
⊟Regulation[edit source | edit]
- sigma factor:
- regulator:
⊟Transcription pattern[edit source | edit]
⊟Protein synthesis (provided by Aureolib)[edit source | edit]
- Aureolib: no data available
⊟Protein stability[edit source | edit]
- half-life: no data available
⊟Biological Material[edit source | edit]
⊟Mutants[edit source | edit]
⊟Expression vector[edit source | edit]
⊟lacZ fusion[edit source | edit]
⊟GFP fusion[edit source | edit]
⊟two-hybrid system[edit source | edit]
⊟FLAG-tag construct[edit source | edit]
⊟Antibody[edit source | edit]
⊟Other Information[edit source | edit]
You are kindly invited to share additional interesting facts.
⊟Literature[edit source | edit]
⊟References[edit source | edit]
- ↑
Roy R Chaudhuri, Andrew G Allen, Paul J Owen, Gil Shalom, Karl Stone, Marcus Harrison, Timothy A Burgis, Michael Lockyer, Jorge Garcia-Lara, Simon J Foster, Stephen J Pleasance, Sarah E Peters, Duncan J Maskell, Ian G Charles
Comprehensive identification of essential Staphylococcus aureus genes using Transposon-Mediated Differential Hybridisation (TMDH).
BMC Genomics: 2009, 10;291
[PubMed:19570206] [WorldCat.org] [DOI] (I e) - ↑
Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
Sci Rep: 2017, 7(1);9718
[PubMed:28887440] [WorldCat.org] [DOI] (I e) - ↑
Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
Proteomics: 2015, 15(21);3648-61
[PubMed:26224020] [WorldCat.org] [DOI] (I p) - ↑
Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
PLoS Genet.: 2016, 12(4);e1005962
[PubMed:27035918] [WorldCat.org] [DOI] (I e)
⊟Relevant publications[edit source | edit]