From AureoWiki
Jump to: navigation, search
NCBI: 26-AUG-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA1391 [new locus tag: SA_RS07865 ]
  • pan locus tag?: SAUPAN004143000
  • symbol: dnaG
  • pan gene symbol?: dnaG
  • synonym:
  • product: DNA primase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA1391 [new locus tag: SA_RS07865 ]
  • symbol: dnaG
  • product: DNA primase
  • replicon: chromosome
  • strand: -
  • coordinates: 1596711..1598528
  • length: 1818
  • essential: yes DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    TTGCGAATAGATCAATCGATCATTAATGAAATAAAAGATAAAACCGACATTTTAGACTTG
    GTAAGTGAATATGTAAAACTAGAAAAGAGAGGACGCAATTATATAGGTTTGTGTCCTTTT
    CATGATGAAAAGACACCTTCATTTACAGTTTCTGAAGATAAACAAATCTGTCATTGTTTT
    GGTTGTAAAAAAGGTGGCAATGTTTTTCAATTTACTCAAGAAATTAAAGACATATCATTT
    GTTGAAGCGGTTAAAGAATTAGGTGATAGAGTTAATGTTGCTGTAGATATTGAGGCAACA
    CAATCTAACTCAAATGTTCAAATTGCTTCTGATGATTTACAAATGATTGAAATGCATGAG
    TTAATACAAGAATTTTATTATTACGCTTTAACAAAGACAGTCGAAGGCGAACAAGCATTA
    ACATACTTACAAGAACGTGGTTTTACAGATGCGCTTATTAAAGAGCGAGGCATTGGCTTT
    GCACCCGATAGCTCACATTTTTGTCATGATTTTCTTCAAAAAAAGGGTTACGATATTGAA
    TTAGCATATGAAGCCGGATTATTATCACGTAACGAAGAAAATTTCAGTTATTACGATAGA
    TTTCGAAATCGTATTATGTTTCCTTTGAAAAATGCGCAAGGAAGAATTGTTGGATATTCA
    GGTCGAACATATACCGGTCAAGAACCAAAATACCTAAATAGTCCTGAAACGCCTATCTTT
    CAAAAAAGAAAGTTGTTATATAACTTAGATAAAGCACGTAAATCAATTAGAAAATTAGAT
    GAAATTGTATTACTAGAAGGTTTTATGGATGTTATAAAATCTGATACTGCTGGCTTGAAA
    AACGTTGTTGCAACAATGGGTACACAGTTGTCAGATGAACATATTACCTTTATACGAAAG
    TTAACATCAAATATAACATTAATGTTTGATGGGGATTTTGCGGGTAGTGAAGCAACACTT
    AAAACAGGTCAACATTTGTTACAGCAAGGGCTAAATGTATTTGTTATACAATTGCCATCT
    GGCATGGATCCGGATGAATACATTGGTAAGTATGGCAACGACGCATTTACTACTTTTGTA
    AAAAATGACAAAAAGTCATTTGCACATTATAAAGTAAGTATATTAAAAGATGAAATTGCA
    CATAATGACCTTTCATATGAACGTTATTTGAAAGAACTGAGTCATGACATTTCACTTATG
    AAGTCATCAATTCTGCAACAAAAGGCTATAAATGATGTTGCGCCATTTTTCAATGTTAGT
    CCTGAGCAGTTAGCTAACGAAATACAATTCAATCAAGCACCAGCCAATTATTATCCAGAA
    GATGAGTATGGCGGTTATGATGAGTATGGCGGTTATATTGAACCTGAGCCAATTGGTATG
    GCACAATTTGACAATTTGAGCCGTCAAGAAAAAGCGGAGCGAGCATTTTTAAAACATTTA
    ATGAGAGATAAAGATACATTTTTAAATTATTATGAAAGTGTTGATAAGGATAACTTCACA
    AATCAGCATTTTAAATATGTATTCGAAGTCTTACATGATTTTTATGCGGAAAATGATCAA
    TATAATATCAGTGATGCTGTGCAGTATGTTAATTCAAATGAGTTGAGAGAAACACTAATT
    AGCTTAGAACAATATAATTTGAATGACGAACCATATGAAAATGAAATTGATGATTATGTC
    AATGTTATTAATGAAAAAGGACAAGAAACAATTGAGTCATTGAATCATAAATTAAGGGAA
    GCTACAAGGATTGGCGATGTAGAATTACAAAAATACTATTTACAGCAAATTGTTGCTAAG
    AATAAAGAACGCATGTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1818

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA1391 [new locus tag: SA_RS07865 ]
  • symbol: DnaG
  • description: DNA primase
  • length: 605
  • theoretical pI: 4.69548
  • theoretical MW: 70031
  • GRAVY: -0.58562

Function[edit source | edit]

  • reaction:
    EC 2.7.7.-?  ExPASy
  • TIGRFAM:
    Genetic information processingDNA metabolismDNA replication, recombination, and repairDNA primase (TIGR01391; EC 2.7.7.-; HMM-score: 507.9)
    Unknown functionGeneralDNA primase-like protein (TIGR00646; HMM-score: 34.2)
    Genetic information processingDNA metabolismDNA replication, recombination, and repairreplicative DNA helicase (TIGR00665; EC 3.6.4.12; HMM-score: 21.1)
    Genetic information processingTranscriptionRNA processingribonuclease M5 (TIGR00334; EC 3.1.26.8; HMM-score: 17.9)
    Genetic information processingMobile and extrachromosomal element functionsProphage functionsphage replicative helicase, DnaB family (TIGR03600; HMM-score: 13.2)
  • TheSEED:  
    DNA MetabolismDNA replicationDNA-replication DNA primase (EC 2.7.7.-) 
  • PFAM:
    no clan definedToprim_N; DNA primase catalytic core, N-terminal domain (PF08275; HMM-score: 139.3)
    Zn_Beta_Ribbon (CL0167) zf-CHC2; CHC2 zinc finger (PF01807; HMM-score: 129.9)
    Toprim-like (CL0413) Toprim_2; Toprim-like (PF13155; HMM-score: 54.3)
    Toprim_4; Toprim domain (PF13662; HMM-score: 53.1)
    no clan definedDnaB; DnaB-like helicase N terminal domain (PF00772; HMM-score: 26.5)
    Toprim-like (CL0413) Toprim_3; Toprim domain (PF13362; HMM-score: 26.5)
    Toprim; Toprim domain (PF01751; HMM-score: 16.8)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors: Mg2+, Zn2+
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.109
    • Ymax_pos: 60
    • Cmax: 0.107
    • Cmax_pos: 60
    • Smax: 0.148
    • Smax_pos: 47
    • Smean: 0.083
    • D: 0.099
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MRIDQSIINEIKDKTDILDLVSEYVKLEKRGRNYIGLCPFHDEKTPSFTVSEDKQICHCFGCKKGGNVFQFTQEIKDISFVEAVKELGDRVNVAVDIEATQSNSNVQIASDDLQMIEMHELIQEFYYYALTKTVEGEQALTYLQERGFTDALIKERGIGFAPDSSHFCHDFLQKKGYDIELAYEAGLLSRNEENFSYYDRFRNRIMFPLKNAQGRIVGYSGRTYTGQEPKYLNSPETPIFQKRKLLYNLDKARKSIRKLDEIVLLEGFMDVIKSDTAGLKNVVATMGTQLSDEHITFIRKLTSNITLMFDGDFAGSEATLKTGQHLLQQGLNVFVIQLPSGMDPDEYIGKYGNDAFTTFVKNDKKSFAHYKVSILKDEIAHNDLSYERYLKELSHDISLMKSSILQQKAINDVAPFFNVSPEQLANEIQFNQAPANYYPEDEYGGYDEYGGYIEPEPIGMAQFDNLSRQEKAERAFLKHLMRDKDTFLNYYESVDKDNFTNQHFKYVFEVLHDFYAENDQYNISDAVQYVNSNELRETLISLEQYNLNDEPYENEIDDYVNVINEKGQETIESLNHKLREATRIGDVELQKYYLQQIVAKNKERM

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]

Scott A Koepsell, Marilynn A Larson, Christopher A Frey, Steven H Hinrichs, Mark A Griep
Staphylococcus aureus primase has higher initiation specificity, interacts with single-stranded DNA stronger, but is less stimulated by its helicase than Escherichia coli primase.
Mol. Microbiol.: 2008, 68(6);1570-82
[PubMed:18452509] [WorldCat.org] [DOI] (I p)