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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01691
  • pan locus tag?: SAUPAN004174000
  • symbol: SAOUHSC_01691
  • pan gene symbol?: comEC
  • synonym:
  • product: DNA internalization-related competence protein ComEC/Rec2

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_01691
  • symbol: SAOUHSC_01691
  • product: DNA internalization-related competence protein ComEC/Rec2
  • replicon: chromosome
  • strand: -
  • coordinates: 1601433..1603580
  • length: 2148
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    GTGCTCTCTACATTTCTTTTCATTTTACTTTTGTATATTACTTATCGTAAAAATAAAATC
    GTTTATGCCCCTATTTCTCTCTTTTTAATCATTTTCTCCGCATGGTATTTACATTATTCA
    CAACAAGCAATATTTAATTATATCAATTATATTGAACGTAATTCTCAGTTTAATGAGCGT
    GCTCAAGTAATCCAAATTCAACGTCAAGGTAGTGACACATATAAAGGTAGGTTGAGTTTA
    AAAAATGAAATATATCCTTTCTTTTTAACAGATAAAAAGAATTTTGATTTAAAGAAAATT
    GAAAGTCGTAATTGTATTGTTAAAGGACAATTCAAAGTTAATGACAATAAGTTTGTAACT
    CTTAAATTACAAAGTATAGTTGTACAAAGCTGCCTAGAATCGAACCGGTCTAATTTAATT
    GAGAAACATAAACAGTTTATAATGAATCGAATTTATGATTCGGGTATTAAGTTTCCGGAT
    CGTATTATGGCATTGATTACTGGTGACGTAAAAGAAGTTAATGAGCAATTTAAGGAACGT
    GTTAAAGAGATAGGTATATATCATTTGCTGGCAGTTAGTGGCTCGCATATAGCTGCAATT
    GTATTTTTAATTTACCAACCTTTAAAACGATTAAATTTACCTTTATTTGTCATTAAAGGA
    ATTACAATCATTGTATTAGCTTTATTTGCTCAATACACAAATTATGCACCTAGTGCTGTA
    AGAGCTATAATAATGACAACTCTTGTACTGGTTATTACTAAGCAAATTAAAATAAAGGGT
    ATTCAGCTATTAGCATTTGCATTTATAATTATGTTTATTTTAAATCCACTAGTTGTTTAT
    GATATTGGATTTCAATTTTCATTCATCATTTCATTTTTTATTATGCTACTTTTTCCTTTT
    TTACAGCAATTGTCAAAGTTACAATCATTATTCATAATTACGTTTATTGCACAATTAGCT
    TCATTTATCGTTGCCATTCCAAGCTTTCATCAACTTCAATGGGTGGGATTTTTATCTAAT
    TTGATTTTTGTACCGTACTATTCGATTATATTGTTTCCGCTATCTATTTTATTCTTTATT
    ACAAGTCATTTTATTGTGGGATTAACGCCGCTAAATTACTTGGTTGACCTAAGTTTTAAT
    TTTCATGACTGGTTACTAGACCTATTCACAAGAATCAAGCAATCACATTTTTCTGTTCCC
    AAGTTTAATGATTGGATATTTATAGTATTTATAATTTCTGTTTATTACATATTTTGGTTA
    TTGGCTAAACGTAAATATATATTGGTTACGTTTTGGACTATAATTATTCTGACATTATTA
    ATAACGTTTCCAACAAATTCACATCACAAAATTACAATGTTAAATGTGGGGCAGGGAGAC
    AGTATTTTATATGAAGGCGGTAAGAACCAAAATGTCTTGATTGATACAGGTGGGAAAGTG
    ATTGATGATACTAAACAACCTAGTTATTCAATTTCTAAATATCATATTTTACCAACGCTA
    AATGAAAGAGGGATAAATGAATTAGAGTATCTAATTTTAACACATCCACACAATGACCAT
    ATTGGTGAAGTGGAATATATTATTAGTCATATTAAAATTAAACATATAGTGATATACAAT
    AAGGGATATAGTAGTAATACATTGATGTTATTATCGAAATTAAGCCATAAGTACAACATT
    AAACTTATGGATGTAAGACAAGTTAGTAGTTTTAAACTTGGAGATAGTAGTTTTCTATTT
    TTTGATAGTTTTATTCCAAATAGCCGAGATAAAAATGAGTATTCGATTATTACTATGATT
    ACATATCAAAATAAAAAAGTTTTATTAATGGGCGATGCTAGTAAAAATAATGAATCTTTA
    CTACTAAAAAAATATAACTTGCCGGAGATTGATATTTTAAAAGTAGGACATCATGGGAGC
    AAGACAAGTAGTTCTAAAGAATTTATAGAGATGATTAAGCCTAAAATAAGTTTGATTTCT
    TCTGGGAAGAACAATATGTATCATCTTCCTAATATAGAAGTTGTTAAACGATTGCAAAGG
    ATTCGCAGTCGCATTTACAATAGTCAACAAAACGGTCAAGTTACAATTGACTTAGATGAT
    AATTTAAAAGTTGATTCAAGCTCTTATGGAAATGCAAGTGGTTTATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2148

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_01691
  • symbol: SAOUHSC_01691
  • description: DNA internalization-related competence protein ComEC/Rec2
  • length: 715
  • theoretical pI: 10.0045
  • theoretical MW: 82863.8
  • GRAVY: 0.231608

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Cellular processesCellular processesDNA transformationDNA internalization-related competence protein ComEC/Rec2 (TIGR00361; HMM-score: 276.2)
    Unknown functionGeneralComEC/Rec2-related protein (TIGR00360; HMM-score: 76)
    Cellular processesCellular processesDetoxificationhydroxyacylglutathione hydrolase (TIGR03413; EC 3.1.2.6; HMM-score: 17.2)
    arCOG00543 universal archaeal KH-domain/beta-lactamase-domain protein (TIGR03675; HMM-score: 15.8)
  • TheSEED:  
    DNA MetabolismDNA uptake, competenceGram Positive Competence Late competence protein ComEC, DNA transport 
    DNA uptake, competenceLate competence Late competence protein ComEC, DNA transport 
  • PFAM:
    no clan definedCompetence; Competence protein (PF03772; HMM-score: 177.6)
    Metallo-HOrase (CL0381) Lactamase_B; Metallo-beta-lactamase superfamily (PF00753; HMM-score: 80.4)
    Lactamase_B_2; Beta-lactamase superfamily domain (PF12706; HMM-score: 13.9)
    no clan definedDUF4131; Domain of unknown function (DUF4131) (PF13567; HMM-score: 12.6)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 10
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.117
    • Ymax_pos: 11
    • Cmax: 0.127
    • Cmax_pos: 44
    • Smax: 0.182
    • Smax_pos: 1
    • Smean: 0.129
    • D: 0.121
  • predicted transmembrane helices (TMHMM): 8

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MLSTFLFILLLYITYRKNKIVYAPISLFLIIFSAWYLHYSQQAIFNYINYIERNSQFNERAQVIQIQRQGSDTYKGRLSLKNEIYPFFLTDKKNFDLKKIESRNCIVKGQFKVNDNKFVTLKLQSIVVQSCLESNRSNLIEKHKQFIMNRIYDSGIKFPDRIMALITGDVKEVNEQFKERVKEIGIYHLLAVSGSHIAAIVFLIYQPLKRLNLPLFVIKGITIIVLALFAQYTNYAPSAVRAIIMTTLVLVITKQIKIKGIQLLAFAFIIMFILNPLVVYDIGFQFSFIISFFIMLLFPFLQQLSKLQSLFIITFIAQLASFIVAIPSFHQLQWVGFLSNLIFVPYYSIILFPLSILFFITSHFIVGLTPLNYLVDLSFNFHDWLLDLFTRIKQSHFSVPKFNDWIFIVFIISVYYIFWLLAKRKYILVTFWTIIILTLLITFPTNSHHKITMLNVGQGDSILYEGGKNQNVLIDTGGKVIDDTKQPSYSISKYHILPTLNERGINELEYLILTHPHNDHIGEVEYIISHIKIKHIVIYNKGYSSNTLMLLSKLSHKYNIKLMDVRQVSSFKLGDSSFLFFDSFIPNSRDKNEYSIITMITYQNKKVLLMGDASKNNESLLLKKYNLPEIDILKVGHHGSKTSSSKEFIEMIKPKISLISSGKNNMYHLPNIEVVKRLQRIRSRIYNSQQNGQVTIDLDDNLKVDSSSYGNASGL

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]