From AureoWiki
Jump to: navigation, search
NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01836
  • pan locus tag?: SAUPAN004385000
  • symbol: SAOUHSC_01836
  • pan gene symbol?: ptaA
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_01836
  • symbol: SAOUHSC_01836
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1740916..1742382
  • length: 1467
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGTATAAATTTTTCCAAAATTTAGGGCGTTCGTTAATGTTACCAGTTGCCATTTTACCG
    GCTGCAGCAATTATTGCCGGAATTGGTAATACGTTAAATGCACTTCATGCGACGCCGAAA
    ATCGCAATGTTCTTTACTACGGTAGGTACTACGATTTTAGAGCAATTAGGTATTTTATTT
    GCAATTGGTGTAGCTATAGGAATGGCAAAGAAAAATGATGGTGCAGTCGCGCTTGCAGCT
    ACGTTAGGATATTTTTTAGTTACAGTTGTATTATCACCAATGAAATTAGCGCCTTTATTA
    GGAATGAAAGCTTCGGAAATAAATTCAGCTTTTGAAAAGATGAATAATGGCAATGTCTTT
    GTTGGTATTGTAATAGGTTTAATAGCAGCTTATGCATACAATAAGTTTAGTGAAACAGAA
    TTACCATTAGCATTATCATTTTTTAGTGGTAAACGTCTAGTTCCAATTATGACTGCATTT
    TACTGTACATTTTTAGTTGTCATATTGTTATTCTTATGGCCACTACTTTATTCATGGATT
    GTAAAATTTGGTGAATCTATAGTCGGTTTAGGATCGTTTGGAGCTTTCATATATGGTGTT
    GCCAATAGATTATTAATTCCTACAGGATTACATCATGCACTGAATAGTGTGTTCTGGTTT
    GATACGATTGGTATTAATGATATTGGTAAATTCCAAAGTGGCAAAGATGCTATAAAAGGT
    ATTACAGGACGCTATCAAGCGGGATTCTTCCCAATCATGATGTTCGGTATTCCAGCGGCT
    GCGTTAGCAATGTATCATACAGCTAAAACTACTCAGAAAAAACAAGTTTACGGTTGGTTC
    CTAGCGAGTTCAGTTGCAGCATTTTTCGTTGGTGTTACAGAGCCGATTGAGTTTGCATTT
    ATGTTTGTTGTGCCAATCTTATATGTTGTTCATGCGTTATTAACAGGATTATCTTTATTT
    ATCGCAGCTACATTCCATTGGACAGCTGGATTTTCATTTAGTGCAGGTTTGATAGATTAT
    GTGTTATCACTAATTAATCCAGTATCAAATCATCCATTGATGTTGCTTGTTCAAGGTGTT
    GTATTTTTCATCTTATACTATGTGATTTTCCGTGTAGTAATCCAAGTATTTAACTTGAAT
    ACGATTGGTAGAGGTGAAAATGAATTAGTTGACCCAACAGTTGTAAAAGATAATATTGCT
    CCTGGTGAAAATGATATTAAACAAAGTAAATATCATCAACATGCTATACAAATATTAGAA
    GGTTTAGGCGGTCAAGAGAATATTGTTAATTTAACCAATTGTGCAACAAGGTTGCGTCTA
    GAGTTAAAAGACACATCTATTATTGATAAACAGAAGATTAAAAACGCCGGTGCAGTTGGT
    GTTACTGTTAATGGCAAACATTCAACTCAAGTTATTGTCGGTACACACGTACAGCAGGTT
    GCTGATGAAATTGAAAAACATTTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1467

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_01836
  • symbol: SAOUHSC_01836
  • description: hypothetical protein
  • length: 488
  • theoretical pI: 9.38912
  • theoretical MW: 53114.3
  • GRAVY: 0.630123

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsPTS system, N-acetylglucosamine-specific IIBC component (TIGR01998; EC 2.7.1.69; HMM-score: 711.1)
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsPTS system, glucose-specific IIBC component (TIGR02002; EC 2.7.1.69; HMM-score: 378.5)
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsPTS system, maltose and glucose-specific IIBC component (TIGR02004; EC 2.7.1.69; HMM-score: 290)
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsPTS system, alpha-glucoside-specific IIBC component (TIGR02005; EC 2.7.1.69; HMM-score: 228.7)
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsPTS system, IIBC component (TIGR02003; EC 2.7.1.69; HMM-score: 194.3)
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsPTS system, maltose and glucose-specific subfamily, IIC component (TIGR00852; HMM-score: 175.7)
    Signal transductionSignal transductionPTSPTS system, maltose and glucose-specific subfamily, IIC component (TIGR00852; HMM-score: 175.7)
    PTS system, sucrose-specific IIBC component (TIGR01996; EC 2.7.1.69; HMM-score: 58.4)
    PTS system, beta-glucoside-specific IIABC component (TIGR01995; EC 2.7.1.69; HMM-score: 56.1)
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsPTS system, glucose-like IIB component (TIGR00826; EC 2.7.1.69; HMM-score: 46.2)
    Signal transductionSignal transductionPTSPTS system, glucose-like IIB component (TIGR00826; EC 2.7.1.69; HMM-score: 46.2)
    PTS system, trehalose-specific IIBC component (TIGR01992; EC 2.7.1.69; HMM-score: 44.7)
  • TheSEED:  
    Chitin and N-acetylglucosamine utilization PTS system, N-acetylglucosamine-specific IIB component (EC 2.7.1.69) / PTS system, N-acetylglucosamine-specific IIC component 
    Sialic Acid Metabolism PTS system, N-acetylglucosamine-specific IIB component (EC 2.7.1.69) / PTS system, N-acetylglucosamine-specific IIC component 
  • PFAM:
    PTS_EIIC (CL0493) PTS_EIIC; Phosphotransferase system, EIIC (PF02378; HMM-score: 232.3)
    no clan definedPTS_EIIB; phosphotransferase system, EIIB (PF00367; HMM-score: 57.2)
    Oxa1 (CL0376) 60KD_IMP; 60Kd inner membrane protein (PF02096; HMM-score: 10.4)
    no clan definedSH; Viral small hydrophobic protein (PF01445; HMM-score: 7.1)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 11
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: 0.5
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.283
    • Ymax_pos: 24
    • Cmax: 0.248
    • Cmax_pos: 38
    • Smax: 0.634
    • Smax_pos: 13
    • Smean: 0.545
    • D: 0.385
  • predicted transmembrane helices (TMHMM): 11

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MYKFFQNLGRSLMLPVAILPAAAIIAGIGNTLNALHATPKIAMFFTTVGTTILEQLGILFAIGVAIGMAKKNDGAVALAATLGYFLVTVVLSPMKLAPLLGMKASEINSAFEKMNNGNVFVGIVIGLIAAYAYNKFSETELPLALSFFSGKRLVPIMTAFYCTFLVVILLFLWPLLYSWIVKFGESIVGLGSFGAFIYGVANRLLIPTGLHHALNSVFWFDTIGINDIGKFQSGKDAIKGITGRYQAGFFPIMMFGIPAAALAMYHTAKTTQKKQVYGWFLASSVAAFFVGVTEPIEFAFMFVVPILYVVHALLTGLSLFIAATFHWTAGFSFSAGLIDYVLSLINPVSNHPLMLLVQGVVFFILYYVIFRVVIQVFNLNTIGRGENELVDPTVVKDNIAPGENDIKQSKYHQHAIQILEGLGGQENIVNLTNCATRLRLELKDTSIIDKQKIKNAGAVGVTVNGKHSTQVIVGTHVQQVADEIEKHL

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  2. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]