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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01839
  • pan locus tag?: SAUPAN004388000
  • symbol: SAOUHSC_01839
  • pan gene symbol?: tyrS
  • synonym:
  • product: tyrosyl-tRNA synthetase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_01839
  • symbol: SAOUHSC_01839
  • product: tyrosyl-tRNA synthetase
  • replicon: chromosome
  • strand: -
  • coordinates: 1744667..1745929
  • length: 1263
  • essential: yes [1] DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGACGAATGTATTAATTGAAGATTTAAAATGGAGAGGTCTTATTTATCAACAAACTGAT
    GAACAAGGTATTGAAGATTTATTAAATAAAGAACAAGTGACGTTATACTGCGGTGCCGAT
    CCAACGGCAGATAGTTTACATATTGGTCACTTACTACCATTCTTAACATTAAGACGTTTT
    CAAGAACATGGACATCGTCCTATCGTTTTAATTGGCGGTGGTACAGGTATGATTGGTGAT
    CCATCAGGTAAATCAGAAGAACGTGTGCTACAAACAGAAGAACAAGTAGATAAAAATATC
    GAAGGTATTAGTAAGCAAATGCACAATATTTTTGAATTTGGAACAGACCATGGTGCAGTG
    CTTGTTAATAATAGAGACTGGTTAGGACAAATCTCATTAATTAGTTTTTTACGTGACTAT
    GGTAAACACGTCGGCGTTAATTACATGTTAGGTAAAGATTCAATCCAAAGTCGTTTAGAA
    CATGGTATTTCATATACAGAATTCACATACACGATTTTACAAGCTATTGATTTCGGTCAT
    TTGAATAGAGAATTGAATTGTAAGATTCAAGTAGGTGGATCAGATCAATGGGGTAATATC
    ACAAGTGGTATTGAATTAATGCGTCGTATGTATGGTCAAACAGACGCATACGGTTTAACT
    ATTCCGCTTGTAACTAAATCAGATGGTAAGAAATTTGGTAAGTCTGAGTCAGGTGCTGTT
    TGGTTAGATGCTGAAAAAACAAGTCCTTATGAATTTTATCAATTCTGGATTAATCAATCA
    GACGAAGATGTAATTAAATTCTTAAAATACTTTACTTTCTTAGGAAAAGAAGAAATTGAT
    CGCTTAGAACAATCTAAAAATGAAGCACCGCATTTACGTGAAGCTCAAAAAACATTAGCT
    GAAGAAGTAACTAAATTTATTCATGGTGAAGATGCATTAAATGATGCAATCCGTATTTCA
    CAAGCATTATTTAGTGGTGATTTAAAATCATTATCAGCGAAAGAATTAAAAGATGGATTT
    AAAGATGTGCCTCAAGTGACATTATCAAATGACACAACAAATATCGTTGAAGTCCTTATT
    GAAACAGGCATTTCTCCTTCTAAACGACAAGCACGTGAAGATGTTAACAATGGTGCGATT
    TATATTAATGGTGAGAGACAACAAGATGTTAATTATGCTTTAGCACCAGAAGATAAAATT
    GATGGCGAATTTACGATTATTCGTCGCGGTAAGAAAAAATACTTCATGGTTAACTATCAA
    TAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1263

Protein[edit source | edit]

Protein Data Bank: 1JII
Protein Data Bank: 1JIJ
Protein Data Bank: 1JIK
Protein Data Bank: 1JIL

General[edit source | edit]

  • locus tag: SAOUHSC_01839
  • symbol: SAOUHSC_01839
  • description: tyrosyl-tRNA synthetase
  • length: 420
  • theoretical pI: 5.05025
  • theoretical MW: 47597.2
  • GRAVY: -0.488333

Function[edit source | edit]

  • reaction:
    EC 6.1.1.1?  ExPASy
    Tyrosine--tRNA ligaseATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr)
  • TIGRFAM:
    Genetic information processingProtein synthesistRNA aminoacylationtyrosine--tRNA ligase (TIGR00234; EC 6.1.1.1; HMM-score: 446.5)
    Genetic information processingProtein synthesistRNA aminoacylationtryptophan--tRNA ligase (TIGR00233; EC 6.1.1.2; HMM-score: 13.7)
    Genetic information processingProtein synthesisRibosomal proteins: synthesis and modificationribosomal protein uS4 (TIGR01017; HMM-score: 12.7)
  • TheSEED:  
    Tyrosyl-tRNA synthetase (EC 6.1.1.1) 
  • PFAM:
    HUP (CL0039) tRNA-synt_1b; tRNA synthetases class I (W and Y) (PF00579; HMM-score: 284.1)
    S4 (CL0492) S4; S4 domain (PF01479; HMM-score: 34.2)

Structure, modifications & interactions[edit source | edit]

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.098
    • Ymax_pos: 24
    • Cmax: 0.128
    • Cmax_pos: 24
    • Smax: 0.108
    • Smax_pos: 21
    • Smean: 0.064
    • D: 0.085
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MTNVLIEDLKWRGLIYQQTDEQGIEDLLNKEQVTLYCGADPTADSLHIGHLLPFLTLRRFQEHGHRPIVLIGGGTGMIGDPSGKSEERVLQTEEQVDKNIEGISKQMHNIFEFGTDHGAVLVNNRDWLGQISLISFLRDYGKHVGVNYMLGKDSIQSRLEHGISYTEFTYTILQAIDFGHLNRELNCKIQVGGSDQWGNITSGIELMRRMYGQTDAYGLTIPLVTKSDGKKFGKSESGAVWLDAEKTSPYEFYQFWINQSDEDVIKFLKYFTFLGKEEIDRLEQSKNEAPHLREAQKTLAEEVTKFIHGEDALNDAIRISQALFSGDLKSLSAKELKDGFKDVPQVTLSNDTTNIVEVLIETGISPSKRQAREDVNNGAIYINGERQQDVNYALAPEDKIDGEFTIIRRGKKKYFMVNYQ

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SAOUHSC_01839 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 2.13 2.14 2.15

Relevant publications[edit source | edit]