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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01876
  • pan locus tag?: SAUPAN004444000
  • symbol: SAOUHSC_01876
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_01876
  • symbol: SAOUHSC_01876
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1791523..1792704
  • length: 1182
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGAATATACCTAAATCAGTCTGGTGGCTAGTAATTGGCATGGCGTTAAATATTACTGGT
    TCCAGTTTTTTGTGGCCTTTAAATACAATTTATATGAAACAAGAACTTGGAAAAAGTTTA
    ACTGTTGCTGGTTTAGTGCTAATGATAAATTCATTTGGCATGGTTATTGGAAACTTATTA
    GGTGGTTCACTATTTGATAAATTAGGTGGATACAAGACGATTTTAATTGGAACTTTCACT
    TGTCTTTGTAGTACAACGCTACTTAATTTCTTTCACGGGTGGCCTTGGTATGCTGTATGG
    CTTGTAATGTTAGGGTTTGGTGGCGGAATGATTATTCCTGCGATATACGCTATGGCTGGA
    GCAGTGTGGCCAAATGGCGGAAGACAAACGTTTAATGCGATATACTTAGCGCAAAATATT
    GGTGTGGCTGTCGGTGCTGCAATGGGCGGCTTTGTCGCAGAATTTAGCTTTAACTATATC
    TTTTTAGCCAATCTTATTATGTATGTTGTGTTTGCGCTTGTCGCGGTAACGCAATTTAAT
    ATTGAAATTAATGCGAAAGTTAAATATCCAACTCATTTAGATATTACTGGTAAAAAGAAT
    AAAGCAAGATTTATTTCATTAGTACTAATTTGTGCAATGTTTGCAATTTGTTGGGTTGCA
    TATATTCAATGGGAGTCTACAATCGCTTCATTTACACAATCTATTAATATTTCAATGGCA
    CAATATAGTGTTTTATGGACAATTAACGGAATAATGATTTTAGTAGCACAACCATTAATT
    AAACCGATTCTCTATCTGTTAAAAGGAAACTTAAAGAAGCAAATGTTTGTCGGCATCATC
    ATTTTTATGTTGTCGTTCTTTGTCACGAGTTTTGCCGAAAACTTTACAATATTTGTTGTC
    GGTATGATTATTTTAACTTTTGGAGAAATGTTTGTATGGCCAGCAGTTCCAACTATAGCC
    AATCAGTTAGCGCCAGATGGTAAGCAAGGACAGTACCAAGGTTTTGTGAATTCAGCTGCT
    ACAGTAGGAAAAGCATTTGGTCCATTTCTTGGTGGTGTATTAGTTGATGCGTTTAATATG
    CGCATGATGTTTATCGGTATGATGCTACTACTTGTATTTGCATTAATATTATTAATGGTT
    TTCAAGGAGAATAATACGCAACCTAAAAAAATAGATGCATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1182

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_01876
  • symbol: SAOUHSC_01876
  • description: hypothetical protein
  • length: 393
  • theoretical pI: 9.75148
  • theoretical MW: 43344.8
  • GRAVY: 0.840204

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    multidrug resistance protein (TIGR00880; HMM-score: 72.2)
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsD-galactonate transporter (TIGR00893; HMM-score: 69.8)
    Cellular processesCellular processesToxin production and resistancedrug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 48.1)
    MetabolismTransport and binding proteinsOtherdrug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 48.1)
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsphosphoglycerate transporter family protein (TIGR00881; HMM-score: 31)
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsMFS transporter, aromatic acid:H+ symporter (AAHS) family (TIGR00895; HMM-score: 30.5)
    MetabolismTransport and binding proteinsCations and iron carrying compoundsH+ Antiporter protein (TIGR00900; HMM-score: 27)
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsMFS transporter, sialate:H+ symporter (SHS) family (TIGR00891; HMM-score: 26.8)
    drug resistance transporter, Bcr/CflA subfamily (TIGR00710; HMM-score: 21.8)
    synaptic vesicle protein SV2 (TIGR01299; HMM-score: 4.3)
  • TheSEED:  
    Multidrug resistance protein 
  • PFAM:
    MFS (CL0015) MFS_1; Major Facilitator Superfamily (PF07690; HMM-score: 141.1)
    MFS_1_like; MFS_1 like family (PF12832; HMM-score: 39.8)
    Sugar_tr; Sugar (and other) transporter (PF00083; HMM-score: 12.8)
    no clan definedTrp_oprn_chp; Tryptophan-associated transmembrane protein (Trp_oprn_chp) (PF09534; HMM-score: 12.3)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 12
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.148
    • Ymax_pos: 18
    • Cmax: 0.146
    • Cmax_pos: 25
    • Smax: 0.255
    • Smax_pos: 6
    • Smean: 0.192
    • D: 0.165
  • predicted transmembrane helices (TMHMM): 12

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MNIPKSVWWLVIGMALNITGSSFLWPLNTIYMKQELGKSLTVAGLVLMINSFGMVIGNLLGGSLFDKLGGYKTILIGTFTCLCSTTLLNFFHGWPWYAVWLVMLGFGGGMIIPAIYAMAGAVWPNGGRQTFNAIYLAQNIGVAVGAAMGGFVAEFSFNYIFLANLIMYVVFALVAVTQFNIEINAKVKYPTHLDITGKKNKARFISLVLICAMFAICWVAYIQWESTIASFTQSINISMAQYSVLWTINGIMILVAQPLIKPILYLLKGNLKKQMFVGIIIFMLSFFVTSFAENFTIFVVGMIILTFGEMFVWPAVPTIANQLAPDGKQGQYQGFVNSAATVGKAFGPFLGGVLVDAFNMRMMFIGMMLLLVFALILLMVFKENNTQPKKIDA

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-3661
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]