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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01897
  • pan locus tag?: SAUPAN004470000
  • symbol: SAOUHSC_01897
  • pan gene symbol?: sigS
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_01897
  • symbol: SAOUHSC_01897
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 1808335..1808805
  • length: 471
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    TTGAAATTTAATGACGTATACAACAAACACCAGAAAATCATACACTATCTTTTAAAAAAA
    TATAATATTAGCTATAATTATGATGAGTATTATCAACTACTCTTGATAAAAATGTGGCAA
    TTGAGTCAGATATATAAACCCTCAAGCAAGCAATCTTTATCCTCTTTTTTATTCACTCGA
    TTAAATTTTTACCTTATCGATTTATTCAGACAACAAAATCAATTAAAAGATGTCATTTTA
    TGTGAGAATAATTCACCAACATTAACTGAACAACCAACATACTTTAATGAACATGACCTT
    CGTTTACAAGATATCTTCAAGCTTTTAAATCAAAGAGAAAGACTATGGCTCAAACTATAC
    CTTGAAGGATACAAGCAATTTGAAATTGCTGAAATCATGTCATTATCGCTTTCAACGATT
    AAATTAATTAAGATGTCCGTTAAGCGTAAATGCCAACATAATTTTAATTAG
    60
    120
    180
    240
    300
    360
    420
    471

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_01897
  • symbol: SAOUHSC_01897
  • description: hypothetical protein
  • length: 156
  • theoretical pI: 9.76636
  • theoretical MW: 19153.2
  • GRAVY: -0.469231

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    RNA polymerase sigma factor, sigma-70 family (TIGR02937; HMM-score: 60.9)
    transcriptional regulator BotR, P-21 (TIGR03209; HMM-score: 28.8)
    RNA polymerase sigma factor, SigZ family (TIGR02959; HMM-score: 28)
    RNA polymerase sigma-70 factor, Bacteroides expansion family 1 (TIGR02985; HMM-score: 22.3)
    RNA polymerase sigma factor, FliA/WhiG family (TIGR02479; HMM-score: 21.8)
    RNA polymerase sigma-70 factor, TIGR02954 family (TIGR02954; HMM-score: 16.6)
    RNA polymerase sigma factor, TIGR02999 family (TIGR02999; HMM-score: 15)
    LuxR family transcriptional regulatory, chaperone HchA-associated (TIGR03541; HMM-score: 14.9)
    Cellular processesCellular processesSporulation and germinationRNA polymerase sigma-G factor (TIGR02850; HMM-score: 12.4)
    Genetic information processingTranscriptionTranscription factorsRNA polymerase sigma-G factor (TIGR02850; HMM-score: 12.4)
    RNA polymerase sigma-B factor (TIGR02941; HMM-score: 12.1)
    RNA polymerase sigma-70 factor, sigma-E family (TIGR02983; HMM-score: 11.9)
  • TheSEED:  
    RNA polymerase sigma factor SigS 
  • PFAM:
    HTH (CL0123) Sigma70_r2; Sigma-70 region 2 (PF04542; HMM-score: 28.7)
    GerE; Bacterial regulatory proteins, luxR family (PF00196; HMM-score: 24.3)
    Sigma70_r4; Sigma-70, region 4 (PF04545; HMM-score: 20.1)
    Sigma70_r4_2; Sigma-70, region 4 (PF08281; HMM-score: 19.4)
    Terminase_5; Putative ATPase subunit of terminase (gpP-like) (PF06056; HMM-score: 13.4)
    HTH_7; Helix-turn-helix domain of resolvase (PF02796; HMM-score: 12.9)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.116
    • Ymax_pos: 12
    • Cmax: 0.115
    • Cmax_pos: 51
    • Smax: 0.197
    • Smax_pos: 8
    • Smean: 0.138
    • D: 0.125
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MKFNDVYNKHQKIIHYLLKKYNISYNYDEYYQLLLIKMWQLSQIYKPSSKQSLSSFLFTRLNFYLIDLFRQQNQLKDVILCENNSPTLTEQPTYFNEHDLRLQDIFKLLNQRERLWLKLYLEGYKQFEIAEIMSLSLSTIKLIKMSVKRKCQHNFN

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]

Lindsey N Shaw, Catharina Lindholm, Tomasz K Prajsnar, Halie K Miller, Melanie C Brown, Ewa Golonka, George C Stewart, Andrej Tarkowski, Jan Potempa
Identification and characterization of sigma, a novel component of the Staphylococcus aureus stress and virulence responses.
PLoS ONE: 2008, 3(12);e3844
[PubMed:19050758] [WorldCat.org] [DOI] (I p)