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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01942
  • pan locus tag?: SAUPAN004567000
  • symbol: SAOUHSC_01942
  • pan gene symbol?: splA
  • synonym:
  • product: serine protease SplA

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_01942
  • symbol: SAOUHSC_01942
  • product: serine protease SplA
  • replicon: chromosome
  • strand: -
  • coordinates: 1846654..1847361
  • length: 708
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGAATAAAAATGTAATGGTTAAAGGTTTAACTGCTTTAACTATTTTAACATCTCTTGGT
    TTTGCTGAAAATATTTCTAATCAGCCTCATTCAATTGCCAAAGCAGAAAAGAATGTCAAA
    GAAATTACCGATGCAACTAAGGAACCATACAATTCAGTGGTAGCATTTGTGGGTGGTACT
    GGTGTAGTTGTTGGTAAAAATACAATCGTAACTAACAAACATATCGCTAAAAGTAATGAT
    ATTTTTAAAAATAGAGTATCAGCACATCATTCGAGTAAAGGTAAAGGCGGAGGAAACTAC
    GACGTTAAAGACATTGTAGAATATCCCGGAAAAGAAGACCTTGCGATAGTTCATGTTCAT
    GAAACAAGTACAGAAGGTTTGAATTTTAATAAGAACGTTAGTTATACAAAATTTGCAGAC
    GGAGCAAAAGTGAAAGATAGAATTTCTGTTATTGGTTATCCAAAGGGTGCACAAACAAAA
    TATAAAATGTTTGAATCGACAGGAACGATTAACCATATCAGTGGAACGTTTATGGAATTT
    GATGCGTATGCACAACCAGGTAATTCAGGATCTCCTGTATTGAATTCTAAACATGAACTG
    ATTGGTATTTTATATGCAGGTAGTGGAAAAGATGAATCTGAAAAGAATTTCGGTGTTTAT
    TTCACACCACAATTAAAAGAATTTATTCAAAATAATATTGAAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    708

⊟Protein[edit | edit source]

Protein Data Bank: 2W7S
Protein Data Bank: 2W7U
Protein Data Bank: 3UFA
Protein Data Bank: 4MVN

⊟General[edit | edit source]

  • locus tag: SAOUHSC_01942
  • symbol: SAOUHSC_01942
  • description: serine protease SplA
  • length: 235
  • theoretical pI: 9.51102
  • theoretical MW: 25548.6
  • GRAVY: -0.431064

⊟Function[edit | edit source]

  • reaction:
    EC 3.4.21.19?  ExPASy
    Glutamyl endopeptidase Preferential cleavage: Glu-|-Xaa, Asp-|-Xaa
  • TIGRFAM:
    Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides periplasmic serine peptidase DegS (TIGR02038; EC 3.4.21.-; HMM-score: 29.1)
    Signal transduction Regulatory functions Protein interactions periplasmic serine peptidase DegS (TIGR02038; EC 3.4.21.-; HMM-score: 29.1)
    Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides peptidase Do (TIGR02037; EC 3.4.21.-; HMM-score: 24.4)
    Genetic information processing Protein fate Protein folding and stabilization peptidase Do (TIGR02037; EC 3.4.21.-; HMM-score: 24.4)
  • TheSEED  :
    • serine protease
  • PFAM:
    Peptidase_PA (CL0124) Trypsin; Trypsin (PF00089; HMM-score: 73.9)
    Trypsin_2; Trypsin-like peptidase domain (PF13365; HMM-score: 59.5)
    and 5 more
    Peptidase_S46; Peptidase S46 (PF10459; HMM-score: 24.5)
    Peptidase_S32; Equine arteritis virus serine endopeptidase S32 (PF05579; HMM-score: 20.4)
    Peptidase_S7; Peptidase S7, Flavivirus NS3 serine protease (PF00949; HMM-score: 19.4)
    DUF31; Putative peptidase (DUF31) (PF01732; HMM-score: 16.4)
    Peptidase_S39; Peptidase S39 (PF02122; HMM-score: 12.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Extracellular
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0.09
    • Cellwall Score: 0.18
    • Extracellular Score: 9.73
    • Internal Helices: 0
  • LocateP: Secretory(released) (with CS)
    • Prediction by SwissProt Classification: Extracellular
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: 0.5
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: NISNQPHS
  • SignalP: Signal peptide SP(Sec/SPI) length 35 aa
    • SP(Sec/SPI): 0.917726
    • TAT(Tat/SPI): 0.012326
    • LIPO(Sec/SPII): 0.019476
    • Cleavage Site: CS pos: 35-36. AKA-EK. Pr: 0.5793
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MNKNVMVKGLTALTILTSLGFAENISNQPHSIAKAEKNVKEITDATKEPYNSVVAFVGGTGVVVGKNTIVTNKHIAKSNDIFKNRVSAHHSSKGKGGGNYDVKDIVEYPGKEDLAIVHVHETSTEGLNFNKNVSYTKFADGAKVKDRISVIGYPKGAQTKYKMFESTGTINHISGTFMEFDAYAQPGNSGSPVLNSKHELIGILYAGSGKDESEKNFGVYFTPQLKEFIQNNIEK

⊟Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: SaeR* (activation) regulon
    SaeR*(TF)important in Virulence; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ 1.0 1.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

S B Reed, C A Wesson, L E Liou, W R Trumble, P M Schlievert, G A Bohach, K W Bayles
Molecular characterization of a novel Staphylococcus aureus serine protease operon.
Infect Immun: 2001, 69(3);1521-7
[PubMed:11179322] [WorldCat.org] [DOI] (P p)Grzegorz M Popowicz, Grzegorz Dubin, Justyna Stec-Niemczyk, Anna Czarny, Adam Dubin, Jan Potempa, Tad A Holak
Functional and structural characterization of Spl proteases from Staphylococcus aureus.
J Mol Biol: 2006, 358(1);270-9
[PubMed:16516230] [WorldCat.org] [DOI] (P p)Justyna Stec-Niemczyk, Katarzyna Pustelny, Magdalena Kisielewska, Michal Bista, Kevin T Boulware, Henning R Stennicke, Ida B Thogersen, Patrick S Daugherty, Jan J Enghild, Krzysztof Baczynski, Grzegorz M Popowicz, Adam Dubin, Jan Potempa, Grzegorz Dubin
Structural and functional characterization of SplA, an exclusively specific protease of Staphylococcus aureus.
Biochem J: 2009, 419(3);555-64
[PubMed:19175361] [WorldCat.org] [DOI] (I p)

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