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Pangenome04-0298108BA0217611819-9771193COLECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50N315NCTC8325NewmanRF122ST398T0131TCH60TW20USA300_FPR3757USA300_TCH1516VC40
NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01949
  • pan locus tag?: SAUPAN004575000
  • symbol: SAOUHSC_01949
  • pan gene symbol?: epiP
  • synonym:
  • product: intracellular serine protease

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_01949
  • symbol: SAOUHSC_01949
  • product: intracellular serine protease
  • replicon: chromosome
  • strand: -
  • coordinates: 1851533..1852906
  • length: 1374
  • essential: no DEG

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGAAAATCATAAAGCGCGCTATTATTAGTTTAATAATATTGAGTCTTTTAATATCTATT
    ACAATGAGTAATGCATCAGCGTCAGAAGAACTATATTACAGTGTTGAATACAAAAATACA
    GCAACATTTAACAAGTTAGTTAAAAAGAAGTCCTTAAACGTTGTCTATAATATACCGGAA
    TTACATGTGGCACAGATTAAAATGACGAAAATGCATGCTAATGCTTTAGCAAACTATAAA
    AATGATATTAAATATATCAATGCCACATGTTCAACTTGTATTACTAGCGAGAAAACAATA
    GACAGAACATCAAATGAGTCATTATTTTCAAGACAATGGGATATGAATAAAATAACCAAT
    AATGGTGCATCGTATGATGATTTGCCAAAACATGCTAACACCAAAATAGCAATCATAGAT
    ACAGGTGTGATGAAAAACCATGACGATTTGAAAAATAATTTCTCGACTGATTCTAAAAAT
    TTAGTACCTTTAAACGGTTTTAGAGGTACTGAACCGGAGGAAACAGGTGATGTTCACGAT
    GTCAATGATAGGAAAGGACATGGCACGATGGTGTCGGGTCAAACGAGTGCTAATGGTAAG
    TTAATAGGTGTTGCACCGAATAACAAATTTACAATGTATCGCGTGTTTGGTAGTAAAAAA
    ACAGAACTGCTTTGGGTATCAAAAGCGATTGTTCAAGCTGCAAATGATGGAAATCAAGTC
    ATTAATATTAGTGTTGGTAGTTATATTATTTTGGACAAAAATGACCATCAAACATTTAGA
    AAAGATGAAAAAGTAGAATACGATGCGTTACAGAAAGCAATCAATTACGCCAAGAAGAAA
    AAATCTATCGTTGTTGCTGCAGCTGGTAATGATGGTATTGATGTCAATGACAAACAGAAA
    CTAAAATTACAGCGTGAATATCAAGGTAATGGCGAAGTGAAAGATGTTCCTGCATCTATG
    GACAATGTCGTTACAGTAGGATCTACAGATCAAAAGAGTAATCTATCTGAGTTTTCCAAT
    TTTGGTATGAATTATACAGATATTGCTGCGCCCGGAGGATCATTTGCTTATTTAAATCAA
    TTCGGTGTGGATAAATGGATGAATGAAGGGTATATGCATAAGGAGAACATTTTAACTACT
    GCCAATAACGGAAGATATATTTATCAAGCTGGAACTTCATTAGCCACACCTAAAGTTTCG
    GGAGCACTAGCTTTAATCATTGATAAATATCATCTTGAAAAACATCCAGATAAAGCGATT
    GAATTGTTATATCAGCATGGGACATCTAAGAATAATAAACCATTTAGTAGATATGGGCAT
    GGTGAGCTTGATGTGTATAAAGCATTAAATGTAGCAAATCAAAAAGCAAGTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1374

Protein[edit source | edit]

Protein Data Bank: 3QFH
Protein Data Bank: 3T41

General[edit source | edit]

  • locus tag: SAOUHSC_01949
  • symbol: SAOUHSC_01949
  • description: intracellular serine protease
  • length: 457
  • theoretical pI: 9.48761
  • theoretical MW: 50735.1
  • GRAVY: -0.497812

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Genetic information processingProtein fateProtein and peptide secretion and traffickingtype VII secretion-associated serine protease mycosin (TIGR03921; EC 3.4.21.-; HMM-score: 136)
    Genetic information processingProtein fateProtein modification and repairtype VII secretion-associated serine protease mycosin (TIGR03921; EC 3.4.21.-; HMM-score: 136)
    cyanobactin maturation protease, PatA/PatG family (TIGR03895; EC 3.4.21.-; HMM-score: 50.3)
  • TheSEED:  
    Epidermin leader peptide processing serine protease epiP precursor (EC 3.4.21.-) 
  • PFAM:
    no clan definedPeptidase_S8; Subtilase family (PF00082; HMM-score: 146.4)
    DUF2850; Protein of unknown function (DUF2850) (PF11012; HMM-score: 13.1)
    DUF2960; Protein of unknown function (DUF2960) (PF11173; HMM-score: 12.5)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Extracellular
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0.09
    • Cellwall Score: 0.18
    • Extracellular Score: 9.73
    • Internal Helices: 0
  • LocateP: Secretory(released) (with CS)
    • Prediction by SwissProt Classification: Extracellular
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: 1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: SNASASEE
  • SignalP: Signal peptide length 28 aa
    • Ymax: 0.794
    • Ymax_pos: 28
    • Cmax: 0.811
    • Cmax_pos: 28
    • Smax: 0.899
    • Smax_pos: 13
    • Smean: 0.778
    • D: 0.787
  • predicted transmembrane helices (TMHMM): 1

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MKIIKRAIISLIILSLLISITMSNASASEELYYSVEYKNTATFNKLVKKKSLNVVYNIPELHVAQIKMTKMHANALANYKNDIKYINATCSTCITSEKTIDRTSNESLFSRQWDMNKITNNGASYDDLPKHANTKIAIIDTGVMKNHDDLKNNFSTDSKNLVPLNGFRGTEPEETGDVHDVNDRKGHGTMVSGQTSANGKLIGVAPNNKFTMYRVFGSKKTELLWVSKAIVQAANDGNQVINISVGSYIILDKNDHQTFRKDEKVEYDALQKAINYAKKKKSIVVAAAGNDGIDVNDKQKLKLQREYQGNGEVKDVPASMDNVVTVGSTDQKSNLSEFSNFGMNYTDIAAPGGSFAYLNQFGVDKWMNEGYMHKENILTTANNGRYIYQAGTSLATPKVSGALALIIDKYHLEKHPDKAIELLYQHGTSKNNKPFSRYGHGELDVYKALNVANQKAS

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]