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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02000
  • pan locus tag?: SAUPAN004844000
  • symbol: SAOUHSC_02000
  • pan gene symbol?: gsaB
  • synonym:
  • product: glutamate-1-semialdehyde aminotransferase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_02000
  • symbol: SAOUHSC_02000
  • product: glutamate-1-semialdehyde aminotransferase
  • replicon: chromosome
  • strand: +
  • coordinates: 1909259..1910548
  • length: 1290
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGAATTTTAGTGAAAGTGAACGTTTACAACAACTTTCAAACGAATATATTCTAGGCGGT
    GTCAATTCCCCTTCTCGTTCTTATAAAGCTGTAGGAGGCGGTGCACCTGTTGTTATGAAA
    GAAGGACACGGTGCATATTTATATGATGTCGATGGCAATAAATTTATTGATTACCTTCAA
    GCATACGGTCCAATTATTACGGGGCATGCACATCCTCATATTACTAAAGCAATTCAAGAA
    CAAGCTGCTAAAGGTGTTTTATTTGGTACACCGACTGAATTAGAAATTGAATTCAGCAAA
    AAATTACGTGATGCAATTCCATCTCTTGAGAAAATTCGCTTTGTAAATTCTGGAACAGAA
    GCAGTCATGACAACAATTCGTGTTGCACGTGCATATACTAAAAGAAATAAAATTATAAAA
    TTTGCTGGATCTTATCATGGCCATTCTGATTTAGTATTGGTTGCAGCAGGTAGCGGCCCA
    TCTCAGCTCGGTTCTCCAGACTCAGCTGGTGTTCCAGAAAGCGTCGCACGTGAAGTCATT
    ACTGTACCTTTCAATGATATTAACGCCTATAAAGAAGCAATTGAATTTTGGGGTGATGAA
    ATTGCCGCAGTATTAGTAGAACCAATTGTTGGTAACTTTGGAATGGTAATGCCTCAACCT
    GGATTTTTAGAAGAGGTTAATGAAATTTCACATAACAATGGGACCCTAGTGATTTATGAT
    GAAGTAATTACTGCATTCCGTTTCCATTACGGTGCCGCTCAAGATTTATTAGGTGTTATC
    CCTGATTTAACTGCATTTGGTAAAATTGTTGGCGGTGGTTTACCAATTGGAGGCTATGGT
    GGACGTCAAGATATTATGGAACAAGTAGCACCTCTAGGACCTGCATATCAAGCTGGTACA
    ATGGCTGGTAACCCGTTATCTATGAAAGCAGGTATTGCATTACTCGAAGTACTAGAACAA
    GACGGTGTTTATGAAAAATTAGACAGCTTAGGCCAACAACTAGAAGAAGGTTTACTTAAA
    TTAATCGAAAAACATAATATCACAGCTACAATTAATCGTATTTATGGATCTTTAACATTG
    TACTTTACAGATGAAAAAGTCACACATTATGATCAAGTTGAACATTCTGACGGCGAAGCG
    TTCGGTAAATTTTTCAAATTAATGTTAAATCAAGGTATCAATTTAGCACCTTCTAAGTTT
    GAAGCTTGGTTCTTAACAACTGAACATACAGAAGAAGATATTAAACAAACTTTAAAAGCT
    GCAGACTATGCTTTTAGTCAAATGAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1290

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_02000
  • symbol: SAOUHSC_02000
  • description: glutamate-1-semialdehyde aminotransferase
  • length: 429
  • theoretical pI: 4.90976
  • theoretical MW: 46755.7
  • GRAVY: -0.12028

Function[edit source | edit]

  • reaction:
    EC 5.4.3.8?  ExPASy
    Glutamate-1-semialdehyde 2,1-aminomutase(S)-4-amino-5-oxopentanoate = 5-aminolevulinate
  • TIGRFAM:
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersHeme, porphyrin, and cobalaminglutamate-1-semialdehyde-2,1-aminomutase (TIGR00713; EC 5.4.3.8; HMM-score: 547.9)
    transaminase, acetylornithine/succinylornithine family (TIGR00707; HMM-score: 175.2)
    MetabolismCentral intermediary metabolismOther4-aminobutyrate transaminase (TIGR00700; EC 2.6.1.19; HMM-score: 159.2)
    ornithine--oxo-acid transaminase (TIGR01885; EC 2.6.1.13; HMM-score: 155.8)
    MetabolismEnergy metabolismAmino acids and aminessuccinylornithine transaminase family (TIGR03246; EC 2.6.1.81; HMM-score: 133.3)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersBiotinadenosylmethionine-8-amino-7-oxononanoate transaminase (TIGR00508; EC 2.6.1.62; HMM-score: 129.4)
    MetabolismCentral intermediary metabolismPolyamine biosynthesisputrescine aminotransferase (TIGR03372; EC 2.6.1.82; HMM-score: 111.8)
    MetabolismCentral intermediary metabolismOther2,4-diaminobutyrate 4-transaminase (TIGR00709; EC 2.6.1.-; HMM-score: 108.3)
    Cellular processesCellular processesAdaptations to atypical conditionsdiaminobutyrate--2-oxoglutarate aminotransferase (TIGR02407; EC 2.6.1.76; HMM-score: 87.1)
    L-lysine 6-transaminase (TIGR03251; EC 2.6.1.36; HMM-score: 64.9)
    MetabolismCentral intermediary metabolismOther4-aminobutyrate aminotransferase (TIGR00699; EC 2.6.1.19; HMM-score: 43.5)
  • TheSEED:  
    Cofactors, Vitamins, Prosthetic Groups, PigmentsTetrapyrrolesCPO analysis Glutamate-1-semialdehyde 2,1-aminomutase (EC 5.4.3.8) 
    CBSS-196164.1.peg.461 Glutamate-1-semialdehyde 2,1-aminomutase (EC 5.4.3.8) 
  • PFAM:
    PLP_aminotran (CL0061) Aminotran_3; Aminotransferase class-III (PF00202; HMM-score: 243.8)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors: pyridoxal 5'-phosphate
  • effectors:
  • protein partners:
    SAOUHSC_02500(rplE)50S ribosomal protein L5  [1] (data from MRSA252)
    SAOUHSC_02492(rplO)50S ribosomal protein L15  [1] (data from MRSA252)
    SAOUHSC_02506(rpsC)30S ribosomal protein S3  [1] (data from MRSA252)
    SAOUHSC_00069protein A  [1] (data from MRSA252)
    SAOUHSC_00530elongation factor Tu  [1] (data from MRSA252)
    SAOUHSC_00795glyceraldehyde-3-phosphate dehydrogenase  [1] (data from MRSA252)
    SAOUHSC_007982,3-bisphosphoglycerate-independent phosphoglycerate mutase  [1] (data from MRSA252)
    SAOUHSC_01490DNA-binding protein HU  [1] (data from MRSA252)
    SAOUHSC_02441alkaline shock protein 23  [1] (data from MRSA252)

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.144
    • Ymax_pos: 30
    • Cmax: 0.141
    • Cmax_pos: 30
    • Smax: 0.194
    • Smax_pos: 28
    • Smean: 0.135
    • D: 0.141
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MNFSESERLQQLSNEYILGGVNSPSRSYKAVGGGAPVVMKEGHGAYLYDVDGNKFIDYLQAYGPIITGHAHPHITKAIQEQAAKGVLFGTPTELEIEFSKKLRDAIPSLEKIRFVNSGTEAVMTTIRVARAYTKRNKIIKFAGSYHGHSDLVLVAAGSGPSQLGSPDSAGVPESVAREVITVPFNDINAYKEAIEFWGDEIAAVLVEPIVGNFGMVMPQPGFLEEVNEISHNNGTLVIYDEVITAFRFHYGAAQDLLGVIPDLTAFGKIVGGGLPIGGYGGRQDIMEQVAPLGPAYQAGTMAGNPLSMKAGIALLEVLEQDGVYEKLDSLGQQLEEGLLKLIEKHNITATINRIYGSLTLYFTDEKVTHYDQVEHSDGEAFGKFFKLMLNQGINLAPSKFEAWFLTTEHTEEDIKQTLKAADYAFSQMK

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)
  2. Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  4. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

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