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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02674
  • pan locus tag?: SAUPAN005920000
  • symbol: SAOUHSC_02674
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_02674
  • symbol: SAOUHSC_02674
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 2457900..2458640
  • length: 741
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    TTGGCATACACATACACTTTAAAAGATATTATTGAAATTACAGGTGTAACTAAAAGAACT
    TTACATTATTACGATGAAATAGGATTATTAGTTCCAGATAAAAATGATAAAAATTATCGC
    GTTTATAAACAGCAAGACTTAGAAAAATTACAAAAGATTTTAATACTCAAGTCTTTTGAT
    TTTGATATCGCTAAAATAAAACAATACATTTCGTATGATAATGAACAATTGCGAAAGTTA
    TTATCAGAGCAAATAAGCAAGTTAGATAAAAAGATTTCTGACTTACAATTAATTAGACGC
    TCTGTATGTGAATTTATTAAAGGACTCTCTCTAATAGATACCAGCATTTTAAACAAGACA
    CTACAGTCACAATATGATAAAGAAGCATCTATAAAATATGGTCATACGAAAGCATATCAA
    TCATTTATTAGACGTAAAGACAGCTTACAATCGCAGGATATCAGACATAAATTGACAACT
    ATCTTCAATAAATTTAATCATATGTCTTTGAGTCATTATCCAATCCAAGATTGTAGTGAT
    CTCGTATTTGAGTGGAAGGCATTTATGAACACTATCGCTGATTTTGATGATGAAACATTA
    TGCTGTATTGCTAAAACATATGAAGATGATACGCGTTTCAAAGATTACTTTAATTCATAT
    GATAATCAAAATTTAGCATCATACATTTCAGAAGCTGTTAATTATTTTTTGAGCAATGTG
    AATAAGAGCGACAATTTTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    741

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_02674
  • symbol: SAOUHSC_02674
  • description: hypothetical protein
  • length: 246
  • theoretical pI: 7.48621
  • theoretical MW: 29126.9
  • GRAVY: -0.555285

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Signal transductionRegulatory functionsDNA interactionsCu(I)-responsive transcriptional regulator (TIGR02044; HMM-score: 44.6)
    Signal transductionRegulatory functionsDNA interactionsCd(II)/Pb(II)-responsive transcriptional regulator (TIGR02047; HMM-score: 40.5)
    Cellular processesCellular processesDetoxificationHg(II)-responsive transcriptional regulator (TIGR02051; HMM-score: 39.6)
    Signal transductionRegulatory functionsDNA interactionsHg(II)-responsive transcriptional regulator (TIGR02051; HMM-score: 39.6)
    Signal transductionRegulatory functionsDNA interactionsZn(II)-responsive transcriptional regulator (TIGR02043; HMM-score: 36.7)
    Cellular processesCellular processesDetoxificationredox-sensitive transcriptional activator SoxR (TIGR01950; HMM-score: 21.3)
    Signal transductionRegulatory functionsDNA interactionsredox-sensitive transcriptional activator SoxR (TIGR01950; HMM-score: 21.3)
  • TheSEED:  
    Virulence, Disease and DefenseResistance to antibiotics and toxic compoundsCobalt-zinc-cadmium resistance Transcriptional regulator, MerR family 
  • PFAM:
    HTH (CL0123) MerR_1; MerR HTH family regulatory protein (PF13411; HMM-score: 60.7)
    no clan definedTipAS; TipAS antibiotic-recognition domain (PF07739; HMM-score: 53.8)
    HTH (CL0123) MerR; MerR family regulatory protein (PF00376; HMM-score: 42.3)
    MerR-DNA-bind; MerR, DNA binding (PF09278; HMM-score: 23.1)
    no clan definedMKT1_N; Temperature dependent protein affecting M2 dsRNA replication (PF12247; HMM-score: 14.6)
    FliD_N; Flagellar hook-associated protein 2 N-terminus (PF02465; HMM-score: 12.5)
    Ribosomal_L12_N; Ribosomal protein L7/L12 dimerisation domain (PF16320; HMM-score: 12.5)
    HTH (CL0123) MerR_2; MerR HTH family regulatory protein (PF13591; HMM-score: 11.7)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.094
    • Ymax_pos: 39
    • Cmax: 0.106
    • Cmax_pos: 39
    • Smax: 0.103
    • Smax_pos: 35
    • Smean: 0.075
    • D: 0.087
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MAYTYTLKDIIEITGVTKRTLHYYDEIGLLVPDKNDKNYRVYKQQDLEKLQKILILKSFDFDIAKIKQYISYDNEQLRKLLSEQISKLDKKISDLQLIRRSVCEFIKGLSLIDTSILNKTLQSQYDKEASIKYGHTKAYQSFIRRKDSLQSQDIRHKLTTIFNKFNHMSLSHYPIQDCSDLVFEWKAFMNTIADFDDETLCCIAKTYEDDTRFKDYFNSYDNQNLASYISEAVNYFLSNVNKSDNF

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  2. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]