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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02679
  • pan locus tag?: SAUPAN005926000
  • symbol: SAOUHSC_02679
  • pan gene symbol?: narJ
  • synonym:
  • product: respiratory nitrate reductase subunit delta

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_02679
  • symbol: SAOUHSC_02679
  • product: respiratory nitrate reductase subunit delta
  • replicon: chromosome
  • strand: -
  • coordinates: 2461774..2462364
  • length: 591
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGGAGGGATTTTCCGTGATTAATTTCGATAATTTTAAAAAATATCAAGAAAGTTTTGGA
    TATATGGCACAACAGTTATGTTTTCCAGAAAAATTAACTTTTCATCCAAAAACTTTTGAA
    GAAACAATTTCGAAAGATCATCCAGCATATGAAGATTTAGTAGCATTTAGAAATGTAATG
    ATGACATTTTCATTATCAGAAATCAAAGCGATTTATACAGATACATTTGATTTTACGAAA
    AAAGCACCATTATATATGACGTATAACAAATTTGATACGCAAAAAGAACGTGGGCAAATG
    TTAGCTAAATTAAAGGTATTATATGAAATGTTTGGACTAGAAATGGTAGATAATGAACTT
    TCTGATTATCTACCGTTGATGCTTCAATTTTTGCAAGTGGCAGAATGGCGAAATGATCCA
    AGAGCTGAAGAGAATATTCAATTAATCATTATGATTATTGAGGATGGAACATATGTGATG
    GCGAATGCACTTGGTGAAGATAACAATCCATATGCCTATGTGATTCAAGCACTTAGAAAA
    ACATTGAAATTATGTTTGACATCACCAAAAGGAGTGAAACATCATGCTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    591

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_02679
  • symbol: SAOUHSC_02679
  • description: respiratory nitrate reductase subunit delta
  • length: 196
  • theoretical pI: 5.09662
  • theoretical MW: 22925.3
  • GRAVY: -0.286735

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Genetic information processingProtein fateProtein folding and stabilizationnitrate reductase molybdenum cofactor assembly chaperone (TIGR00684; HMM-score: 65.6)
    and 1 more
    DMSO reductase family type II enzyme chaperone (TIGR03482; HMM-score: 26.6)
  • TheSEED:  
    Nitrogen MetabolismNitrogen Metabolism - no subcategoryNitrate and nitrite ammonification Respiratory nitrate reductase delta chain (EC 1.7.99.4) 
  • PFAM:
    no clan definedNitrate_red_del; Nitrate reductase delta subunit (PF02613; HMM-score: 25.2)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.109
    • Ymax_pos: 37
    • Cmax: 0.119
    • Cmax_pos: 21
    • Smax: 0.144
    • Smax_pos: 31
    • Smean: 0.083
    • D: 0.099
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MEGFSVINFDNFKKYQESFGYMAQQLCFPEKLTFHPKTFEETISKDHPAYEDLVAFRNVMMTFSLSEIKAIYTDTFDFTKKAPLYMTYNKFDTQKERGQMLAKLKVLYEMFGLEMVDNELSDYLPLMLQFLQVAEWRNDPRAEENIQLIIMIIEDGTYVMANALGEDNNPYAYVIQALRKTLKLCLTSPKGVKHHA

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  2. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]