From AureoWiki
Jump to: navigation, search

NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02740
  • pan locus tag?: SAUPAN005996000
  • symbol: SAOUHSC_02740
  • pan gene symbol?:
  • synonym:
  • product: drug transporter

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_02740
  • symbol: SAOUHSC_02740
  • product: drug transporter
  • replicon: chromosome
  • strand: -
  • coordinates: 2519028..2520428
  • length: 1401
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGACACATCAAAACAATTTAGAGGTGACAACCGATTGCTTTTGGGTATCGTTTTAGGG
    GTTATTACCTTTTGGCTATTCGCGCAGTCACTTGTTAATCTTGTTGTCCCATTACAATCA
    ACATATAGTAGTGACGTTGGAACGATAAATATCGCTGTTAGCTTATCTGCCTTATTTGCT
    GGTTTGTTTATCGTAGGTGCTGGTGATGTTGCTGATAAATTTGGTCGCGTCAAAATTACT
    TATGTAGGATTGATATTAAATGTTGTAGGTTCATTACTCATCATCATTACACCTTTGCCA
    GCATTTTTAATTATAGGTAGAATAATTCAAGGTTTGTCTGCAGCATGTATTATGCCATCA
    ACACTTGCTATTATTAACGAATATTATATTGGTACAAGAAGACAACGTGCCTTAAGCTAT
    TGGTCTATTGGTTCTTGGGGTGGTAGTGGTATTTGTACGTTGTTTGGTGGCTTAATGGCT
    ACATATATAGGTTGGCGTTCAATATTTGTTGTTTCAATTCTATTAACATTATTAGCAATG
    TACTTAATCAAACATGCACCTGAGACTAAAGCAGAACCAATCAAAGGTATGAAAGCAGAA
    GCTAAAAAGTTTGACGTTATTGGTTTAGTCATTTTAGTAGTGACGATGTTAAGTTTAAAT
    GTAATCATCACACAGACGTCTCATTTTGGTTTAGTTTCACCGTTAATTCTAGGTTTAATT
    GTTGTGTTTATCTGTTCATTAGTTGGTTTTGTATATTACGAAAATAAAATAAAGCATCCA
    CTTGTTGATTTTTCAATTTTTAAAAATAGAGGATACAGTGGTGCAACTATTTCAAACTTC
    TTATTAAATGGTGTAGCAGGTGGTGCACTTATCGTTATTAACACGTATTATCAACAACAA
    TTAGGATTTAATTCTTCGCAAACGGGTTATATTTCATTAACGTATTTAATAACAGTGTTG
    TCAATGATTCGTGTAGGTGAAAAGATTTTATCTCAACATGGTCCGAAGCGCCCACTATTA
    CTAGGAAGTGGCTTTACAGTGATTGGGTTAATCTTATTGTCGTTAACATTTTTACCAGAA
    GTGTGGTATATCATATCTAGTATAGTTGGATATTTATTGTTTGGTACTGGTTTAGGATTA
    TATGCTACACCATCAACTGATACAGCAGTTGCTAGTGCGCCAGATGATAAGTCGGGTGTT
    GCTTCAGGTGTGTATAAAATGGCGTCATCATTAGGAAATGCATTTGGAGTAGCAGTATCT
    GGTACGGTTTATACTGTGTTAGCAGCTAATTTAAATTTGAACTTAGGTGGTTTCACAGGT
    ATGATGTTTAATGCCTTGCTAGCAATTGTTGCATTTTTAGTCATTTTACTATTAGTTCCT
    AAAAATCAAACGAATTTGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1401

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_02740
  • symbol: SAOUHSC_02740
  • description: drug transporter
  • length: 466
  • theoretical pI: 9.74613
  • theoretical MW: 49840.8
  • GRAVY: 0.840987

Function[edit source | edit]

  • TIGRFAM:
    Cellular processesCellular processesToxin production and resistancedrug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 123.8)
    MetabolismTransport and binding proteinsOtherdrug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 123.8)
    and 9 more
    multidrug resistance protein (TIGR00880; HMM-score: 61.1)
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsMFS transporter, aromatic acid:H+ symporter (AAHS) family (TIGR00895; HMM-score: 46.9)
    drug resistance transporter, Bcr/CflA subfamily (TIGR00710; HMM-score: 46)
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsD-galactonate transporter (TIGR00893; HMM-score: 35.7)
    MetabolismTransport and binding proteinsAnionsNa(+)-dependent inorganic phosphate cotransporter (TIGR00894; HMM-score: 30.4)
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsMFS transporter, sialate:H+ symporter (SHS) family (TIGR00891; HMM-score: 29.4)
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsphosphoglycerate transporter family protein (TIGR00881; HMM-score: 24.1)
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsoxalate/formate antiporter family transporter (TIGR00890; HMM-score: 20.3)
    MetabolismTransport and binding proteinsCations and iron carrying compoundscation transport protein (TIGR00898; HMM-score: 17.1)
  • TheSEED:  
    putative transporter 
  • PFAM:
    MFS (CL0015) MFS_1; Major Facilitator Superfamily (PF07690; HMM-score: 160.7)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 14
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.163
    • Ymax_pos: 31
    • Cmax: 0.173
    • Cmax_pos: 31
    • Smax: 0.283
    • Smax_pos: 30
    • Smean: 0.148
    • D: 0.157
  • predicted transmembrane helices (TMHMM): 14

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MDTSKQFRGDNRLLLGIVLGVITFWLFAQSLVNLVVPLQSTYSSDVGTINIAVSLSALFAGLFIVGAGDVADKFGRVKITYVGLILNVVGSLLIIITPLPAFLIIGRIIQGLSAACIMPSTLAIINEYYIGTRRQRALSYWSIGSWGGSGICTLFGGLMATYIGWRSIFVVSILLTLLAMYLIKHAPETKAEPIKGMKAEAKKFDVIGLVILVVTMLSLNVIITQTSHFGLVSPLILGLIVVFICSLVGFVYYENKIKHPLVDFSIFKNRGYSGATISNFLLNGVAGGALIVINTYYQQQLGFNSSQTGYISLTYLITVLSMIRVGEKILSQHGPKRPLLLGSGFTVIGLILLSLTFLPEVWYIISSIVGYLLFGTGLGLYATPSTDTAVASAPDDKSGVASGVYKMASSLGNAFGVAVSGTVYTVLAANLNLNLGGFTGMMFNALLAIVAFLVILLLVPKNQTNL

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  2. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]