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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_2389 [new locus tag: SAUSA300_RS13225 ]
  • pan locus tag?: SAUPAN005996000
  • symbol: SAUSA300_2389
  • pan gene symbol?:
  • synonym:
  • product: putative drug transporter

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAUSA300_2389 [new locus tag: SAUSA300_RS13225 ]
  • symbol: SAUSA300_2389
  • product: putative drug transporter
  • replicon: chromosome
  • strand: -
  • coordinates: 2571924..2573324
  • length: 1401
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGGACACATCAAAACAATTTAGAGGTGACAACCGATTGCTTTTGGGTATCGTTTTAGGG
    GTTATTACCTTTTGGCTATTCGCGCAGTCACTTGTTAATCTTGTTGTCCCATTACAATCA
    ACATATAGTAGTGACGTTGGAACGATAAATATCGCTGTTAGCTTATCTGCCTTATTTGCT
    GGTTTGTTTATCGTAGGTGCTGGTGATGTTGCTGATAAATTTGGTCGCGTCAAAATTACT
    TATGTAGGATTGATATTAAATGTTGTAGGTTCATTACTCATCATCATTACACCTTTGCCA
    GCATTTTTAATTATAGGTAGAATAATTCAAGGTTTGTCTGCAGCATGTATTATGCCATCA
    ACACTTGCTATTATTAACGAATATTATATTGGTACAAGAAGACAACGTGCCTTAAGCTAT
    TGGTCTATTGGTTCTTGGGGTGGTAGTGGTATTTGTACGTTGTTTGGTGGCTTAATGGCT
    ACATATATAGGTTGGCGTTCAATATTTGTTGTTTCAATTCTATTAACATTATTAGCAATG
    TACTTAATCAAACATGCACCTGAGACTAAAGCAGAACCAATCAAAGGTATGAAAGCAGAA
    GCTAAAAAGTTTGACGTTATTGGTTTAGTCATTTTAGTAGTGACGATGTTAAGTTTAAAT
    GTAATCATCACACAGACGTCTCATTTTGGTTTAGTTTCACCGTTAATTCTAGGTTTAATT
    GTTGTGTTTATCTGTTCATTAGTTGGTTTTGTATATTACGAAAATAAAATAAAGCATCCA
    CTTGTTGATTTTTCAATTTTTAAAAATAGAGGATACAGTGGTGCAACTATTTCAAACTTC
    TTATTAAATGGTGTAGCAGGTGGTGCACTTATCGTTATTAACACGTATTATCAACAACAA
    TTAGGATTTAATTCTTCGCAAACGGGTTATATTTCATTAACGTATTTAATAACAGTGTTG
    TCAATGATTCGTGTAGGTGAAAAGATTTTATCTCAACATGGTCCGAAGCGCCCACTATTA
    CTAGGAAGTGGCTTTACAGTGATTGGGTTAATCTTATTGTCGTTAACATTTTTACCAGAA
    GTGTGGTATATCATATCTAGTATAGTTGGATATTTATTGTTTGGTACTGGTTTAGGATTA
    TATGCTACACCATCAACTGATACAGCAGTTGCTAGTGCGCCAGATGATAAGTCGGGTGTT
    GCTTCAGGTGTGTATAAAATGGCGTCATCATTAGGAAATGCATTTGGAGTAGCAGTATCT
    GGTACGGTTTATACTGTGTTAGCAGCTAATTTAAATTTGAACTTAGGTGGTTTCACAGGT
    ATGATGTTTAATGCCTTGCTAGCAATTGTTGCATTTTTAGTCATTTTACTATTAGTTCCT
    AAAAATCAAACGAATTTGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1401

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAUSA300_2389 [new locus tag: SAUSA300_RS13225 ]
  • symbol: SAUSA300_2389
  • description: putative drug transporter
  • length: 466
  • theoretical pI: 9.74613
  • theoretical MW: 49840.8
  • GRAVY: 0.840987

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Cellular processesCellular processesToxin production and resistancedrug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 123.8)
    MetabolismTransport and binding proteinsOtherdrug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family (TIGR00711; HMM-score: 123.8)
    multidrug resistance protein (TIGR00880; HMM-score: 61.1)
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsMFS transporter, aromatic acid:H+ symporter (AAHS) family (TIGR00895; HMM-score: 46.9)
    drug resistance transporter, Bcr/CflA subfamily (TIGR00710; HMM-score: 46)
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsD-galactonate transporter (TIGR00893; HMM-score: 35.7)
    MetabolismTransport and binding proteinsAnionsNa(+)-dependent inorganic phosphate cotransporter (TIGR00894; HMM-score: 30.4)
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsMFS transporter, sialate:H+ symporter (SHS) family (TIGR00891; HMM-score: 29.4)
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsphosphoglycerate transporter family protein (TIGR00881; HMM-score: 24.1)
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsoxalate/formate antiporter family transporter (TIGR00890; HMM-score: 20.3)
    MetabolismTransport and binding proteinsCations and iron carrying compoundscation transport protein (TIGR00898; HMM-score: 17.1)
  • TheSEED:  
    putative transporter 
  • PFAM:
    MFS (CL0015) MFS_1; Major Facilitator Superfamily (PF07690; HMM-score: 160.7)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 14
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.163
    • Ymax_pos: 31
    • Cmax: 0.173
    • Cmax_pos: 31
    • Smax: 0.283
    • Smax_pos: 30
    • Smean: 0.148
    • D: 0.157
  • predicted transmembrane helices (TMHMM): 14

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MDTSKQFRGDNRLLLGIVLGVITFWLFAQSLVNLVVPLQSTYSSDVGTINIAVSLSALFAGLFIVGAGDVADKFGRVKITYVGLILNVVGSLLIIITPLPAFLIIGRIIQGLSAACIMPSTLAIINEYYIGTRRQRALSYWSIGSWGGSGICTLFGGLMATYIGWRSIFVVSILLTLLAMYLIKHAPETKAEPIKGMKAEAKKFDVIGLVILVVTMLSLNVIITQTSHFGLVSPLILGLIVVFICSLVGFVYYENKIKHPLVDFSIFKNRGYSGATISNFLLNGVAGGALIVINTYYQQQLGFNSSQTGYISLTYLITVLSMIRVGEKILSQHGPKRPLLLGSGFTVIGLILLSLTFLPEVWYIISSIVGYLLFGTGLGLYATPSTDTAVASAPDDKSGVASGVYKMASSLGNAFGVAVSGTVYTVLAANLNLNLGGFTGMMFNALLAIVAFLVILLLVPKNQTNL

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator: CodY
    CodY (TF) important in Amino acid metabolism  RegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]