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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02802
  • pan locus tag?: SAUPAN006097000
  • symbol: SAOUHSC_02802
  • pan gene symbol?: fnbB
  • synonym:
  • product: fibronectin binding protein B

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_02802
  • symbol: SAOUHSC_02802
  • product: fibronectin binding protein B
  • replicon: chromosome
  • strand: -
  • coordinates: 2577879..2580632
  • length: 2754
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGATCGTTGTTGGAATGGGACAAGAAAAAGAAGCTGCAGCATCGGAACAAAACAATACT
    ACAGTAGAGGAAAGTGGGAGTTCAGCTACTGAAAGTAAAGCAAGCGAAACACAAACAACT
    ACAAATAACGTTAATACAATAGATGAAACACAATCATACAGCGCGACATCAACTGAGCAA
    CCATCACAATCAACACAAGTAACAACAGAAGAAGCACCGAAAACTGTGCAAGCACCAAAA
    GTAGAAACTTCGCGAGTTGATTTGCCATCGGAAAAAGTTGCTGATAAGGAAACTACAGGA
    ACTCAAGTTGACATAGCTCAACCAAGTAACGTCTCAGAAATTAAACCAAGAATGAAAAGA
    TCAACTGACGTTACAGCAGTTGCAGAGAAAGAAGTAGTGGAAGAAACTAAAGCGACAGGT
    ACAGATGTAACAAATAAAGTGGAAGTAGAAGAAGGTAGTGAAATTGTAGGACATAAACAA
    GATACGAATGTTGTAAATCCTCATAACGCAGAAAGAGTAACCTTGAAATATAAATGGAAA
    TTTGGAGAAGGAATTAAGGCGGGAGATTATTTTGATTTCACATTAAGCGATAATGTTGAA
    ACTCATGGTATCTCAACACTGCGTAAAGTTCCGGAGATAAAAAGTACAGATGGTCAAGTT
    ATGGCGACAGGAGAAATAATTGGAGAAAGAAAAGTTAGATATACGTTTAAAGAATATGTA
    CAAGAAAAGAAAGATTTAACTGCTGAATTATCTTTAAATCTATTTATTGATCCTACAACA
    GTGACGCAAAAAGGTAACCAAAATGTTGAAGTTAAATTGGGTGAGACTACGGTTAGCAAA
    ATATTTAATATTCAATATTTAGGTGGAGTTAGAGATAATTGGGGAGTAACAGCTAATGGT
    CGAATTGATACTTTAAATAAAGTAGATGGGAAATTTAGTCATTTTGCGTACATGAAACCT
    AACAACCAGTCGTTAAGCTCTGTGACAGTAACTGGTCAAGTAACTAAAGGAAATAAACCA
    GGGGTTAATAATCCAACAGTTAAGGTATATAAACACATTGGTTCAGACGATTTAGCTGAA
    AGCGTATATGCAAAGCTTGATGATGTCAGCAAATTTGAAGATGTGACTGATAATATGAGT
    TTAGATTTTGATACTAATGGTGGTTATTCTTTAAACTTTAATAATTTAGACCAAAGTAAA
    AATTATGTAATAAAATATGAAGGGTATTATGATTCAAATGCTAGCAACTTAGAATTTCAA
    ACACACCTTTTTGGATATTATAACTATTATTATACAAGTAATTTAACTTGGAAAAATGGC
    GTTGCATTTTACTCTAATAACGCTCAAGGCGACGGCAAAGATAAACTAAAGGAACCTATT
    ATAGAACATAGTACTCCTATCGAACTTGAATTTAAATCAGAGCCGCCAGTGGAGAAGCAT
    GAATTGACTGGTACAATCGAAGAAAGTAATGATTCTAAGCCAATTGATTTTGAATATCAT
    ACAGCTGTTGAAGGTGCAGAAGGTCATGCAGAAGGTACCATTGAAACTGAAGAAGATTCT
    ATTCATGTAGACTTTGAAGAATCGACACATGAAAATTCAAAACATCATGCTGATGTTGTT
    GAATATGAAGAAGATACAAACCCAGGTGGTGGTCAGGTTACTACTGAGTCTAACCTAGTT
    GAATTTGACGAAGATTCTACAAAAGGTATTGTAACTGGTGCTGTTAGCGATCATACAACA
    ATTGAAGATACGAAAGAATATACGACTGAAAGTAATCTGATTGAACTAGTAGATGAACTA
    CCTGAAGAACATGGTCAAGCGCAAGGACCAATCGAGGAAATTACTGAAAACAATCATCAT
    ATTTCTCATTCTGGTTTAGGAACTGAAAATGGTCACGGTAATTATGGCGTGATTGAAGAA
    ATCGAAGAAAATAGCCACGTGGATATTAAGAGTGAATTAGGTTACGAAGGTGGCCAAAAT
    AGCGGTAATCAGTCATTTGAGGAAGACACAGAAGAAGATAAACCGAAATATGAACAAGGT
    GGCAATATCGTAGATATCGATTTCGATAGTGTACCTCAAATTCATGGTCAAAATAATGGT
    AACCAATCATTCGAAGAAGATACAGAGAAAGACAAACCTAAGTATGAACAAGGTGGTAAT
    ATCATTGATATCGACTTCGACAGTGTGCCACATATTCACGGATTCAATAAGCACACTGAA
    ATTATTGAAGAAGATACAAATAAAGATAAACCAAATTATCAATTCGGTGGACACAATAGT
    GTTGACTTTGAAGAAGATACACTTCCACAAGTAAGTGGTCATAATGAAGGTCAACAAACG
    ATTGAAGAAGATACAACACCTCCAATCGTGCCACCAACGCCACCGACACCAGAAGTACCA
    AGCGAGCCGGAAACACCAACACCACCGACACCAGAAGTACCAAGCGAGCCGGAAACACCA
    ACACCGCCAACGCCAGAGGTACCAACTGAACCTGGTAAACCAATACCACCTGCTAAAGAA
    GAACCTAAAAAACCTTCTAAACCAGTGGAACAAGGTAAAGTAGTAACACCTGTTATTGAA
    ATCAATGAAAAGGTTAAAGCAGTGGTACCAACTAAAAAAGCACAATCTAAGAAATCTGAA
    CTACCTGAAACAGGTGGAGAAGAATCAACAAACAACGGCATGTTGTTCGGCGGATTATTT
    AGCATTTTAGGTTTAGCGTTATTACGCAGAAATAAAAAGAATCACAAAGCATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2280
    2340
    2400
    2460
    2520
    2580
    2640
    2700
    2754

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_02802
  • symbol: SAOUHSC_02802
  • description: fibronectin binding protein B
  • length: 917
  • theoretical pI: 4.29721
  • theoretical MW: 101024
  • GRAVY: -0.888877

Function[edit source | edit]

  • TIGRFAM:
    Cell structureCell envelopeOtherLPXTG cell wall anchor domain (TIGR01167; HMM-score: 26.5)
  • TheSEED:  
    Virulence, Disease and DefenseAdhesionAdhesins in Staphylococcus Fibronectin binding protein FnbB 
  • PFAM:
    Adhesin (CL0204) SdrG_C_C; C-terminus of bacterial fibrinogen-binding adhesin (PF10425; HMM-score: 133.5)
    no clan definedFn_bind; Fibronectin binding repeat (PF02986; HMM-score: 107.8)
    and 1 more
    Gram_pos_anchor; LPXTG cell wall anchor motif (PF00746; HMM-score: 34.3)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cellwall
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 10
    • Extracellular Score: 0
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 0.83
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -2
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.201
    • Ymax_pos: 35
    • Cmax: 0.247
    • Cmax_pos: 35
    • Smax: 0.355
    • Smax_pos: 11
    • Smean: 0.195
    • D: 0.198
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MIVVGMGQEKEAAASEQNNTTVEESGSSATESKASETQTTTNNVNTIDETQSYSATSTEQPSQSTQVTTEEAPKTVQAPKVETSRVDLPSEKVADKETTGTQVDIAQPSNVSEIKPRMKRSTDVTAVAEKEVVEETKATGTDVTNKVEVEEGSEIVGHKQDTNVVNPHNAERVTLKYKWKFGEGIKAGDYFDFTLSDNVETHGISTLRKVPEIKSTDGQVMATGEIIGERKVRYTFKEYVQEKKDLTAELSLNLFIDPTTVTQKGNQNVEVKLGETTVSKIFNIQYLGGVRDNWGVTANGRIDTLNKVDGKFSHFAYMKPNNQSLSSVTVTGQVTKGNKPGVNNPTVKVYKHIGSDDLAESVYAKLDDVSKFEDVTDNMSLDFDTNGGYSLNFNNLDQSKNYVIKYEGYYDSNASNLEFQTHLFGYYNYYYTSNLTWKNGVAFYSNNAQGDGKDKLKEPIIEHSTPIELEFKSEPPVEKHELTGTIEESNDSKPIDFEYHTAVEGAEGHAEGTIETEEDSIHVDFEESTHENSKHHADVVEYEEDTNPGGGQVTTESNLVEFDEDSTKGIVTGAVSDHTTIEDTKEYTTESNLIELVDELPEEHGQAQGPIEEITENNHHISHSGLGTENGHGNYGVIEEIEENSHVDIKSELGYEGGQNSGNQSFEEDTEEDKPKYEQGGNIVDIDFDSVPQIHGQNNGNQSFEEDTEKDKPKYEQGGNIIDIDFDSVPHIHGFNKHTEIIEEDTNKDKPNYQFGGHNSVDFEEDTLPQVSGHNEGQQTIEEDTTPPIVPPTPPTPEVPSEPETPTPPTPEVPSEPETPTPPTPEVPTEPGKPIPPAKEEPKKPSKPVEQGKVVTPVIEINEKVKAVVPTKKAQSKKSELPETGGEESTNNGMLFGGLFSILGLALLRRNKKNHKA

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  2. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]

T J Foster, M Höök
Surface protein adhesins of Staphylococcus aureus.
Trends Microbiol.: 1998, 6(12);484-8
[PubMed:10036727] [WorldCat.org] (P p)