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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02933
  • pan locus tag?: SAUPAN006314000
  • symbol: SAOUHSC_02933
  • pan gene symbol?: betB
  • synonym:
  • product: betaine aldehyde dehydrogenase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_02933
  • symbol: SAOUHSC_02933
  • product: betaine aldehyde dehydrogenase
  • replicon: chromosome
  • strand: -
  • coordinates: 2699895..2701385
  • length: 1491
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGAACTTTTAAAACATTTATCTCAGCGTCAATATATTGATGGTGAGTGGGTTGAAAGC
    GCGAATAAAAATACAAGAGATATTATCAATCCTTACAATCAAGAAGTGATATTTACGGTT
    TCTGAAGGGACAAAAGAGGATGCAGAACGTGCAATCTTAGCTGCAAGACGTGCGTTTGAG
    TCTGGTGAATGGTCGCAAGAAACTGCTGAAACAAGAGGTAAAAAAGTACGTGCCATCGCG
    GACAAGATTAAAGAACATCGCGAAGCGTTAGCACGATTAGAAACATTAGATACTGGAAAA
    ACGTTAGAAGAATCATATGCAGATATGGATGATATTCATAATGTGTTTATGTATTTTGCT
    GGATTAGCAGATAAAGACGGTGGCGAAATGATTGATTCACCAATTCCAGATACAGAAAGC
    AAAATTGTTAAAGAACCAGTAGGTGTAGTTACACAAATTACACCTTGGAATTATCCGTTA
    TTACAAGCATCATGGAAAATTGCGCCAGCGCTTGCTACGGGTTGTTCACTAGTTATGAAA
    CCAAGTGAAATTACACCATTAACAACAATACGTGTTTTTGAATTAATGGAAGAAGTTGGT
    TTCCCTAAAGGAACAATTAATCTTATTCTAGGTGCAGGTTCTGAAGTTGGTGACGTAATG
    TCAGGTCATAAAGAGGTTGACCTTGTATCATTTACAGGTGGCATTGAGACTGGTAAGCAT
    ATTATGAAAAATGCTGCTAATAATGTTACGAATATTGCCTTGGAACTTGGCGGTAAAAAT
    CCAAACATTATCTTTGATGATGCTGATTTTGAATTGGCAGTAGACCAAGCGTTAAATGGT
    GGATATTTCCATGCAGGTCAAGTTTGTTCAGCAGGATCAAGAATATTAGTACAAAACAGT
    ATTAAAGACAAATTTGAGCAAGCACTTATTGATCGCGTGAAAAAAATCAAATTAGGTAAT
    GGTTTTGATGCTGATACTGAAATGGGACCAGTGATTTCAACAGAACATCGTAATAAGATC
    GAATCTTATATGGATGTAGCTAAAGCAGAAGGCGCAACAATTGCTGTTGGTGGTAAACGT
    CCAGATAGAGATGATTTAAAAGATGGTCTATTCTTCGAGCCAACAGTCATTACAAATTGT
    GATACGTCAATGCGTATTGTACAAGAAGAGGTTTTCGGACCTGTCGTTACTGTAGAAGGC
    TTTGAAACTGAACAAGAAGCGATTCAATTAGCGAATGATTCTATATATGGTTTAGCAGGT
    GCTGTATTTTCTAAAGATATTGGAAAAGCACAACGCGTTGCTAACAAGTTGAAACTTGGA
    ACGGTGTGGATTAATGATTTCCATCCATATTTTGCACAAGCGCCATGGGGTGGATACAAA
    CAATCAGGTATCGGTAGAGAATTAGGCAAAGAAGGCTTAGAAGAGTACCTTGTTTCAAAA
    CACATTTTAACAAATACAAATCCACAATTAGTGAATTGGTTTAGCAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1491

Protein[edit source | edit]

Protein Data Bank: 4MPB
Protein Data Bank: 4MPY
Protein Data Bank: 4NEA
Protein Data Bank: 4NU9
Protein Data Bank: 4Q92
Protein Data Bank: 4QJE
Protein Data Bank: 4QN2
Protein Data Bank: 4QTO

General[edit source | edit]

  • locus tag: SAOUHSC_02933
  • symbol: SAOUHSC_02933
  • description: betaine aldehyde dehydrogenase
  • length: 496
  • theoretical pI: 4.70024
  • theoretical MW: 54622.4
  • GRAVY: -0.306048

Function[edit source | edit]

  • reaction:
    EC 1.2.1.8?  ExPASy
    Betaine-aldehyde dehydrogenaseBetaine aldehyde + NAD+ + H2O = betaine + NADH
  • TIGRFAM:
    Cellular processesCellular processesAdaptations to atypical conditionsbetaine-aldehyde dehydrogenase (TIGR01804; EC 1.2.1.8; HMM-score: 775.3)
    5-carboxymethyl-2-hydroxymuconate semialdehyde dehydrogenase (TIGR02299; EC 1.2.1.60; HMM-score: 483.1)
    MetabolismEnergy metabolismOther2-hydroxymuconic semialdehyde dehydrogenase (TIGR03216; EC 1.2.1.-; HMM-score: 431.5)
    MetabolismCentral intermediary metabolismOthersuccinate-semialdehyde dehydrogenase (TIGR01780; EC 1.2.1.-; HMM-score: 406.5)
    1-pyrroline dehydrogenase (TIGR03374; EC 1.2.1.19; HMM-score: 401.7)
    Unknown functionEnzymes of unknown specificityaldehyde dehydrogenase, Rv0768 family (TIGR04284; EC 1.2.1.-; HMM-score: 385.2)
    MetabolismEnergy metabolismAmino acids and aminesputative delta-1-pyrroline-5-carboxylate dehydrogenase (TIGR01237; EC 1.2.1.88; HMM-score: 313.2)
    putative phosphonoacetaldehyde dehydrogenase (TIGR03250; EC 1.2.1.-; HMM-score: 299.2)
    MetabolismEnergy metabolismAmino acids and aminesmethylmalonate-semialdehyde dehydrogenase (acylating) (TIGR01722; EC 1.2.1.27; HMM-score: 284.2)
    MetabolismEnergy metabolismAmino acids and aminessuccinylglutamate-semialdehyde dehydrogenase (TIGR03240; EC 1.2.1.71; HMM-score: 236.8)
    MetabolismEnergy metabolismAmino acids and aminesdelta-1-pyrroline-5-carboxylate dehydrogenase (TIGR01238; EC 1.2.1.88; HMM-score: 212.6)
    MetabolismEnergy metabolismAmino acids and amines1-pyrroline-5-carboxylate dehydrogenase (TIGR01236; EC 1.2.1.88; HMM-score: 193.8)
    phenylacetic acid degradation protein paaN (TIGR02278; HMM-score: 156.2)
    acetaldehyde dehydrogenase (acetylating) (TIGR02518; EC 1.2.1.10; HMM-score: 51.1)
    phenylacetic acid degradation protein paaN (TIGR02288; HMM-score: 41.3)
  • TheSEED:  
    Stress ResponseOsmotic stressCholine and Betaine Uptake and Betaine Biosynthesis Betaine aldehyde dehydrogenase (EC 1.2.1.8) 
  • PFAM:
    ALDH-like (CL0099) Aldedh; Aldehyde dehydrogenase family (PF00171; HMM-score: 583.2)
    T3SS-Chaperone (CL0419) YscO-like; YscO-like protein (PF16789; HMM-score: 17.1)
    ALDH-like (CL0099) DUF1487; Protein of unknown function (DUF1487) (PF07368; HMM-score: 15.7)
    no clan definedSART-1; SART-1 family (PF03343; HMM-score: 14)
    Hybrid (CL0105) GARS_C; Phosphoribosylglycinamide synthetase, C domain (PF02843; HMM-score: 12.4)
    no clan definedDUF1465; Protein of unknown function (DUF1465) (PF07323; HMM-score: 12.2)
    RPEL; RPEL repeat (PF02755; HMM-score: 10.5)
    FAM25; FAM25 family (PF15825; HMM-score: 6.8)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.117
    • Ymax_pos: 21
    • Cmax: 0.109
    • Cmax_pos: 21
    • Smax: 0.181
    • Smax_pos: 17
    • Smean: 0.109
    • D: 0.114
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MELLKHLSQRQYIDGEWVESANKNTRDIINPYNQEVIFTVSEGTKEDAERAILAARRAFESGEWSQETAETRGKKVRAIADKIKEHREALARLETLDTGKTLEESYADMDDIHNVFMYFAGLADKDGGEMIDSPIPDTESKIVKEPVGVVTQITPWNYPLLQASWKIAPALATGCSLVMKPSEITPLTTIRVFELMEEVGFPKGTINLILGAGSEVGDVMSGHKEVDLVSFTGGIETGKHIMKNAANNVTNIALELGGKNPNIIFDDADFELAVDQALNGGYFHAGQVCSAGSRILVQNSIKDKFEQALIDRVKKIKLGNGFDADTEMGPVISTEHRNKIESYMDVAKAEGATIAVGGKRPDRDDLKDGLFFEPTVITNCDTSMRIVQEEVFGPVVTVEGFETEQEAIQLANDSIYGLAGAVFSKDIGKAQRVANKLKLGTVWINDFHPYFAQAPWGGYKQSGIGRELGKEGLEEYLVSKHILTNTNPQLVNWFSK

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  2. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

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