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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02943
  • pan locus tag?: SAUPAN006323000
  • symbol: SAOUHSC_02943
  • pan gene symbol?: citM
  • synonym:
  • product: citrate transporter

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_02943
  • symbol: SAOUHSC_02943
  • product: citrate transporter
  • replicon: chromosome
  • strand: -
  • coordinates: 2708110..2709480
  • length: 1371
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGAATAGTGATAATATGTGGTTAACAGTAATGGGGCTCATTATTATTATTTCAATTGTA
    GGTTTACTCATTGCCAAAAAGATAAATCCAGTTGTAGGTATGACAATCATACCTTGCTTA
    GGGGCAATGATTTTAGGATATAGTGTGACAGATTTGGTTGGATTTTTTGCTAAAGGGTTA
    GATCAAGTCATCAACGTTGTTATTATGTTTATCTTTGCCATTATTTTCTTTGGCATCATG
    AACGATAGTGGTTTATTCAAGCCGCTTGTCAAACGCTTAATATTAATGACACGAGGCAAT
    GTCGTCATTGTCTGTGCAATGACAGCTTTAATTGGCACAATAGCCCAATTAGATGGGGCC
    GGTGCGGTAACATTTTTGCTTTCTATTCCTGCATTATTACCTTTATATAAAGCGTTAAAT
    ATGAATAAATATTTATTGATTTTACTATTAGCATTAAGCGCGGCGATTATGAACATGGTA
    CCTTGGGGAGGTCCAATGGCTCGTGTAGCTGCAGTGTTAAAAGCCAAAAGTGTCAATGAA
    TTATGGTATGGATTAATACCTATTCAAATAATAGGTTTCATTCTTGTTATGTTGTTTGCG
    GTATATCTTGGATTTAAAGAACAGAAACGTATCAAAAAAGCAATAGAGAGAAATGAATTA
    CCGCAAACACAAGATATAGATGTACATAAATTAGTTGAAGTATATGAACGAGATCAAGAT
    GTAAGGTTTCCTGTAAAAGGACGTGCAAGAACAAAATCATGGATAAAATGGGTGAATACA
    GCTTTAACTTTAGCTGTTATTCTATCGATGTTAATAAATATTGCGCCACCTGAATTTGCA
    TTCATGATAGGTGTTTCGTTGGCACTTGTTATTAATTTTAAATCAGTGGATGAACAAATG
    GAACGATTAAGAGCGCATGCGCCGAATGCATTAATGATGGCTGCAGTGATTATTGCAGCA
    GGTATGTTTTTAGGTGTACTAAATGAAACCGGTATGCTTAAAGCGATTGCGACCAATTTA
    ATCAAAGTGATTCCTGCAGAAGTAGGACCATACTTGCATATTATTGTAGGTTTACTTGGC
    GTACCATTAGATTTACTAACTAGCACAGACGCTTATTATTTTGCTGTGTTACCGATTGTC
    GAACAAACAGCAGGGCAATTTGGTGTACCGTCTGTATCAACAGCTTATTCAATGGTCATA
    GGGAATATTATAGGTACATTTGTCAGCCCATTTTCACCAGCCTTATGGTTGGCAATTGGT
    TTAGCAGAGGCAAACATGGGCACGTATATTAAGTATGCATTCTTTTGGATTTGGGGATTC
    GCTATCGTTATGTTAGTAATTGCAATGTTGATGGGCATTGTGACGATTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1371

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_02943
  • symbol: SAOUHSC_02943
  • description: citrate transporter
  • length: 456
  • theoretical pI: 9.61164
  • theoretical MW: 49771.1
  • GRAVY: 0.952412

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidscitrate transporter (TIGR00784; HMM-score: 279.8)
    Cell structureCell envelopeOtherLPXTG cell wall anchor domain (TIGR01167; HMM-score: 13.3)
  • TheSEED:  
    Citrate/H+ symporter of CitMHS family 
  • PFAM:
    IT (CL0182) CitMHS; Citrate transporter (PF03600; HMM-score: 131.5)
    DcuC; C4-dicarboxylate anaerobic carrier (PF03606; HMM-score: 26.9)
    DctM; Tripartite ATP-independent periplasmic transporter, DctM component (PF06808; HMM-score: 23.4)
    Na_H_antiporter; Na+/H+ antiporter family (PF03553; HMM-score: 21.6)
    Na_H_antiport_2; Na+-H+ antiporter family (PF13726; HMM-score: 18.3)
    no clan definedGram_pos_anchor; LPXTG cell wall anchor motif (PF00746; HMM-score: 11.9)
    Caudo_bapla_RBP; Receptor-binding protein of phage tail base-plate Siphoviridae, head (PF08931; HMM-score: 11)
    LapA_dom; Lipopolysaccharide assembly protein A domain (PF06305; HMM-score: 9.6)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 12
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.163
    • Ymax_pos: 28
    • Cmax: 0.213
    • Cmax_pos: 28
    • Smax: 0.186
    • Smax_pos: 25
    • Smean: 0.117
    • D: 0.145
  • predicted transmembrane helices (TMHMM): 12

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MNSDNMWLTVMGLIIIISIVGLLIAKKINPVVGMTIIPCLGAMILGYSVTDLVGFFAKGLDQVINVVIMFIFAIIFFGIMNDSGLFKPLVKRLILMTRGNVVIVCAMTALIGTIAQLDGAGAVTFLLSIPALLPLYKALNMNKYLLILLLALSAAIMNMVPWGGPMARVAAVLKAKSVNELWYGLIPIQIIGFILVMLFAVYLGFKEQKRIKKAIERNELPQTQDIDVHKLVEVYERDQDVRFPVKGRARTKSWIKWVNTALTLAVILSMLINIAPPEFAFMIGVSLALVINFKSVDEQMERLRAHAPNALMMAAVIIAAGMFLGVLNETGMLKAIATNLIKVIPAEVGPYLHIIVGLLGVPLDLLTSTDAYYFAVLPIVEQTAGQFGVPSVSTAYSMVIGNIIGTFVSPFSPALWLAIGLAEANMGTYIKYAFFWIWGFAIVMLVIAMLMGIVTI

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  2. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]