NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_0403 [new locus tag: SAUSA300_RS02160 ]
• pan locus tag^?: SAUPAN002118000
• symbol: SAUSA300_0403
• pan gene symbol^?: ssl9
• synonym:
• product: superantigen-like protein

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_0403 [new locus tag: SAUSA300_RS02160 ]
• symbol: SAUSA300_0403
• product: superantigen-like protein
• replicon: chromosome
• strand: +
• coordinates: 455485..456183
• length: 699
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3914104 NCBI
• RefSeq: YP_493117 NCBI
• BioCyc: GH3C-399 BioCyc
• MicrobesOnline: 1291918 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGAAATTAACAACGATAGCTAAAGCAACATTAGCATTAGGAATATTAACTACAGGTGTG
  TTTACAGCAGAAAGTCAAACTGGTCACGCGAAAGTAGAACTTGATGAGACACAACGCAAA
  TATTATATCAATATGCTACATCAATACTATTCTGAAGAAAGTTTTGAACCAACAAACATT
  AGTGTTAAAAGCGAAGATTACTATGGCTCTAACGTTTTAAACTTTAAACAACGAAATAAA
  GCTTTTAAAGTATTTTTACTTGGTGACGATAAAAATAAATATAAAGAAAAAACACATGGC
  CTTGATGTCTTTGCAGTACCTGAATTAATAGATATAAAAGGTGGCATATATAGCGTTGGC
  GGTATAACAAAGAAAAATGTGAGATCAGTGTTTGGATTTGTAAGTAATCCAAGTCTACAA
  GTTAAAAAAGTTGATGCTAAAAATGGCTTTTCGATAAACGAGTTGTTTTTTATTCAAAAG
  GAAGAAGTATCATTGAAGGAACTGGACTTTAAAATAAGAAAACTCTTAATCGAAAAATAT
  AGATTGTATAAAGGAACGTCTGATAAAGGTAGAATTGTTATCAATATGAAAGACGAAAAG
  AAGCATGAAATTGATTTAAGTGAAAAATTAAGTTTTGAACGTATGTTTGATGTAATGGAT
  AGTAAGCAAATTAAAAATATTGAAGTGAATTTGAATTAG
  60
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⊟Protein

• 3R2I
  PDB

Protein Data Bank: 3R2I

⊟General

• locus tag: SAUSA300_0403 [new locus tag: SAUSA300_RS02160 ]
• symbol: SAUSA300_0403
• description: superantigen-like protein
• length: 232
• theoretical pI: 9.69341
• theoretical MW: 26675.5
• GRAVY: -0.450431

⊟Function

• TIGRFAM:
• TheSEED:  
  Exotoxin 13 
• PFAM:
  OB_enterotoxin (CL0658) SSL_OB; Staphylococcal superantigen-like OB-fold domain (PF09199; HMM-score: 109.3)
  and 3 more
  Ant-toxin_C (CL0386) Stap_Strp_tox_C; Staphylococcal/Streptococcal toxin, beta-grasp domain (PF02876; HMM-score: 39.6)
  MAP; MAP domain (PF03642; HMM-score: 14.3)
  no clan definedMRP-S33; Mitochondrial ribosomal subunit S27 (PF08293; HMM-score: 12.5)

⊟Structure, modifications & interactions

• domains:
• modifications:
• cofactors:
• effectors:
• protein partners:

⊟Localization

• PSORTb: Extracellular
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0
  • Extracellular Score: 10
  • Internal Helices: 0
• LocateP: N-terminally anchored (No CS)
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular Possibility: -0.17
  • Signal Peptide Possibility: 1
  • N-terminally Anchored Score: 5
  • Predicted Cleavage Site: No CleavageSite
• SignalP: Signal peptide length 31 aa
  • Ymax: 0.512
  • Ymax_pos: 31
  • Cmax: 0.411
  • Cmax_pos: 31
  • Smax: 0.862
  • Smax_pos: 20
  • Smean: 0.704
  • D: 0.587
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87161416 NCBI
• RefSeq: YP_493117 NCBI
• UniProt: A0A0H2XII7 UniProt

⊟Protein sequence

• MKLTTIAKATLALGILTTGVFTAESQTGHAKVELDETQRKYYINMLHQYYSEESFEPTNISVKSEDYYGSNVLNFKQRNKAFKVFLLGDDKNKYKEKTHGLDVFAVPELIDIKGGIYSVGGITKKNVRSVFGFVSNPSLQVKKVDAKNGFSINELFFIQKEEVSLKELDFKIRKLLIEKYRLYKGTSDKGRIVINMKDEKKHEIDLSEKLSFERMFDVMDSKQIKNIEVNLN

⊟Experimental data

• experimentally validated: no data available

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SAUSA300_0403 no polycistronic organisation predicted

⊟Regulation

• sigma factor:
• regulator: SaeR (activation) regulon

  SaeR

  (TF)

  important in Virulence;

  RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟Relevant publications

Stefan Monecke, Peter Slickers, Ralf Ehricht
Assignment of Staphylococcus aureus isolates to clonal complexes based on microarray analysis and pattern recognition.
FEMS Immunol. Med. Microbiol.: 2008, 53(2);237-51
[PubMed:18507678] [WorldCat.org] [DOI] (P p)