NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_0508 [new locus tag: SAUSA300_RS02720 ]
• pan locus tag^?: SAUPAN002288000
• symbol: SAUSA300_0508
• pan gene symbol^?: mcsA
• synonym:
• product: hypothetical protein

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_0508 [new locus tag: SAUSA300_RS02720 ]
• symbol: SAUSA300_0508
• product: hypothetical protein
• replicon: chromosome
• strand: +
• coordinates: 567121..567609
• length: 489
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3915211 NCBI
• RefSeq: YP_493211 NCBI
• MicrobesOnline: 1292023 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGGTGTGTCAAACTTGTGCTGAGGGACACCATCCGTGGAATCAAGCTAATGAACAACCT
  GAATATCAAGAACATCAAGATAATTTCGAAGAAGCATTTGTTGTTAAGCAAATTTTACAA
  CATTTAGCTACGAAACATGGAATTAATTTTCAAGAAGTAGCGTTTAAAGAAGAAAAACGT
  TGCCCATCATGTCATATGACTTTGAAAGATATTGCACATGTTGGTAAATTTGGGTGTGCT
  AATTGTTATGCAACATTTAAAGATGACATCATTGATATCGTCCGCAGAGTTCAAGGTGGA
  CAATTTGAGCACGTTGGAAAGACACCACATTCTTCACATAAAAAGATAGCTTTAAAGCGA
  AAAATCGAAGAAAAGAATGAATATTTGAAAAAACTTATTGAAATCCAAGATTTTGAGGAA
  GCAGCCATTGTTAGAGATGAAATTAAAGCACTAAAAGCTGAGAGTGAGGTGCAACATGAT
  GACGCATAA
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  489
  

⊟Protein

⊟General

• locus tag: SAUSA300_0508 [new locus tag: SAUSA300_RS02720 ]
• symbol: SAUSA300_0508
• description: hypothetical protein
• length: 162
• theoretical pI: 6.42997
• theoretical MW: 18710
• GRAVY: -0.72037

⊟Function

• reaction:
• TIGRFAM:
  Genetic information processingDNA metabolismDNA replication, recombination, and repairexcinuclease ABC subunit B (TIGR00631; EC 3.1.25.-; HMM-score: 14.8)
  MetabolismTransport and binding proteinsCations and iron carrying compoundssodium/hydrogen antiporter (TIGR00844; HMM-score: 12)
• TheSEED:  
  Protein MetabolismProtein degradationProteolysis in bacteria, ATP-dependent Nucleotide excision repair protein, with UvrB/UvrC motif 
• PFAM:
  no clan definedUVR; UvrB/uvrC motif (PF02151; HMM-score: 23)
  Zn_Beta_Ribbon (CL0167) OrfB_Zn_ribbon; Putative transposase DNA-binding domain (PF07282; HMM-score: 16.5)
  Zn_Tnp_IS1595; Transposase zinc-ribbon domain (PF12760; HMM-score: 16.5)
  Zn-ribbon_8; Zinc ribbon domain (PF09723; HMM-score: 11.5)
  Multiheme_cytos (CL0317) Cytochrom_CIII; Class III cytochrome C family (PF02085; HMM-score: 9.6)
  Zn_Beta_Ribbon (CL0167) zinc-ribbons_6; zinc-ribbons (PF07191; HMM-score: 8.1)
  RING (CL0229) Prok-RING_1; Prokaryotic RING finger family 1 (PF14446; HMM-score: 6.4)

⊟Structure, modifications & interactions

• domains:
• modifications:
• cofactors:
• effectors:
• protein partners:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular Possibility: 1
  • Signal Peptide Possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • Ymax: 0.141
  • Ymax_pos: 57
  • Cmax: 0.18
  • Cmax_pos: 57
  • Smax: 0.195
  • Smax_pos: 16
  • Smean: 0.1
  • D: 0.125
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87160592 NCBI
• RefSeq: YP_493211 NCBI
• UniProt: A0A0H2XEU5 UniProt

⊟Protein sequence

• MVCQTCAEGHHPWNQANEQPEYQEHQDNFEEAFVVKQILQHLATKHGINFQEVAFKEEKRCPSCHMTLKDIAHVGKFGCANCYATFKDDIIDIVRRVQGGQFEHVGKTPHSSHKKIALKRKIEEKNEYLKKLIEIQDFEEAAIVRDEIKALKAESEVQHDDA

⊟Experimental data

• experimentally validated: no data available

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: ctsR > SAUSA300_0508 > SAUSA300_0509 > clpC

⊟Regulation

• sigma factor:
• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Other Information

You are kindly invited to share additional interesting facts.