NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_0945 [new locus tag: SAUSA300_RS05080 ]
• pan locus tag^?: SAUPAN003244000
• symbol: SAUSA300_0945
• pan gene symbol^?: menF
• synonym:
• product: isochorismate synthase family protein

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_0945 [new locus tag: SAUSA300_RS05080 ]
• symbol: SAUSA300_0945
• product: isochorismate synthase family protein
• replicon: chromosome
• strand: +
• coordinates: 1033198..1034409
• length: 1212
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3914450 NCBI
• RefSeq: YP_493643 NCBI
• MicrobesOnline: 1292460 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
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  ATGCGTTTGAATGATAATCGAACGTCATATTTTGGCTACAAAGCAATTCAATTATTCAAA
  AATAATTCTAAAAATAAACAATCTATTTTTAAAGACTGGGAAAAATTAAAACATAACATC
  ACATTTATACATCCGCAATCTGAGAAACATCATCTTCGAGTTGTTGGAGGGTTCCAATTT
  TCAAGTCATAAATCAGATGATGAATGGCGAGAGTTTGGACTAAATCATTTTGTATTACCT
  GAAGTTTTAATTTCAACTGATAATAATGGGACATTTTTAACTTATACAGTTAAAAGGGAA
  AGTTTTACTGTTGAGGCATTGAACGATTTAATGGATTTGTTCAACAATATATCGGACATA
  GATGTGGACGAGCAAATTGGGGGAATTACTAGAAATGAAGATATTTATAAAGATGACTGG
  CGTCAACTTGTAGTAGAAGCTATAGAATCTATTAATAATGAAGAAAAAATTGTACTAGCA
  CGTAGACGGTTAATAAAGTTCGATAAAGATATCAGTATTCCATATATTCTAAAGCAAGCA
  TATTCTAAAGAAAAAAACAGTTATATATTCTTGTTAGAATCACAAGATTCTATATTCTTT
  TCACAAACACCTGAACAATTAATAAAGGTCAATAATAAAATACTATCGACTAAAGCTGTA
  GCAGGTACAATTAAACGTTCACAAGATGAGGACGAAGATACAAAAAATGTTGAAGCATTT
  TTAAAAGATAATAAAAACTTAATCGAACATCGATTTGTTGTTGACAGTATTTTACATGAT
  ATTAAACCTTATATCACTGAATTACATTATGATAAGACGCCTAAAATTCTAAAAAATGAT
  CATTTATATCACTTGTACACTGAAATAAAGGCGCCACTGAAGGATGATTCGTATATTAGT
  TTAATTGATAATTTACATCCAACACCTGCTTTAGGTGGCTATCCAAAAGAATTTGCAATG
  GATTTTATTGAGCAGAAAGAATTTGGTACGCGAGGATTATATGGTGCACCGGTTGGCTAT
  ATAGATATATACGATGATTGTGAATTTATTGTTGCAATTCGTTCGATGCTTATTAAGAAA
  GCACAAGCAACTTTATTTGCTGGGTGTGGCATTGTTAAAGATTCTGATCCAGATAGTGAA
  TTGGCAGAAACGAACCTTAAGTTCACACCTATGATGAATGCATTAGGAGTCGATATGAAT
  GGGAAATCATAA
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  1212
  

⊟Protein

⊟General

• locus tag: SAUSA300_0945 [new locus tag: SAUSA300_RS05080 ]
• symbol: SAUSA300_0945
• description: isochorismate synthase family protein
• length: 403
• theoretical pI: 5.63334
• theoretical MW: 46546.5
• GRAVY: -0.473449

⊟Function

• reaction:
  EC 5.4.4.2?  ExPASy
  Isochorismate synthaseChorismate = isochorismate
• TIGRFAM:
  MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersMenaquinone and ubiquinoneisochorismate synthase (TIGR00543; EC 5.4.4.2; HMM-score: 409.7)
  MetabolismAmino acid biosynthesisAromatic amino acid familyanthranilate synthase component I (TIGR01820; EC 4.1.3.27; HMM-score: 152.6)
  MetabolismAmino acid biosynthesisAromatic amino acid familyanthranilate synthase component I (TIGR00564; EC 4.1.3.27; HMM-score: 138.6)
  MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersFolic acidaminodeoxychorismate synthase, component I (TIGR00553; EC 2.6.1.85; HMM-score: 118.9)
  aminodeoxychorismate synthase, component I (TIGR01824; EC 2.6.1.85; HMM-score: 107.3)
  salicylate synthase (TIGR03494; EC 4.1.3.-,5.4.4.2; HMM-score: 89.1)
  MetabolismAmino acid biosynthesisAromatic amino acid familyanthranilate synthase component I (TIGR00565; EC 4.1.3.27; HMM-score: 83.1)
  MetabolismAmino acid biosynthesisAromatic amino acid familyanthranilate synthase (TIGR01815; EC 4.1.3.27; HMM-score: 60.1)
  aminodeoxychorismate synthase (TIGR01823; EC 2.6.1.85; HMM-score: 59.3)
• TheSEED:  
  Cofactors, Vitamins, Prosthetic Groups, PigmentsQuinone cofactorsMenaquinone and Phylloquinone Biosynthesis Menaquinone-specific isochorismate synthase (EC 5.4.4.2) 
  Quinone cofactorsMenaquinone biosynthesis from chorismate via 1,4-dihydroxy-2-naphthoate Menaquinone-specific isochorismate synthase (EC 5.4.4.2) 
  Menaquinone and Phylloquinone Biosynthesis Isochorismate synthase (EC 5.4.4.2) 
• PFAM:
  no clan definedChorismate_bind; chorismate binding enzyme (PF00425; HMM-score: 217.5)
  ATP_synthase (CL0255) vATP-synt_E; ATP synthase (E/31 kDa) subunit (PF01991; HMM-score: 15.8)
  no clan definedSinI; Anti-repressor SinI (PF08671; HMM-score: 9.3)

⊟Structure, modifications & interactions

• domains:
• modifications:
• cofactors:
• effectors:
• protein partners:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular Possibility: 1
  • Signal Peptide Possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • Ymax: 0.154
  • Ymax_pos: 11
  • Cmax: 0.117
  • Cmax_pos: 29
  • Smax: 0.32
  • Smax_pos: 7
  • Smean: 0.254
  • D: 0.193
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87161685 NCBI
• RefSeq: YP_493643 NCBI
• UniProt: A0A0H2XJA7 UniProt

⊟Protein sequence

• MRLNDNRTSYFGYKAIQLFKNNSKNKQSIFKDWEKLKHNITFIHPQSEKHHLRVVGGFQFSSHKSDDEWREFGLNHFVLPEVLISTDNNGTFLTYTVKRESFTVEALNDLMDLFNNISDIDVDEQIGGITRNEDIYKDDWRQLVVEAIESINNEEKIVLARRRLIKFDKDISIPYILKQAYSKEKNSYIFLLESQDSIFFSQTPEQLIKVNNKILSTKAVAGTIKRSQDEDEDTKNVEAFLKDNKNLIEHRFVVDSILHDIKPYITELHYDKTPKILKNDHLYHLYTEIKAPLKDDSYISLIDNLHPTPALGGYPKEFAMDFIEQKEFGTRGLYGAPVGYIDIYDDCEFIVAIRSMLIKKAQATLFAGCGIVKDSDPDSELAETNLKFTPMMNALGVDMNGKS

⊟Experimental data

• experimentally validated: no data available

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SAUSA300_0945 > menD > SAUSA300_0947 > menB

⊟Regulation

• sigma factor:
• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Other Information

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