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NCBI: 26-AUG-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SA0895 [new locus tag: SA_RS05080 ]
  • pan locus tag?: SAUPAN003244000
  • symbol: SA0895
  • pan gene symbol?: menF
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SA0895 [new locus tag: SA_RS05080 ]
  • symbol: SA0895
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 1015154..1016515
  • length: 1362
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGGCTACGGGCGTATTAGAGGACGATATTGTCAAAGAGATATATGGCAGCTCAAAGGAA
    TGGGTTTCAGTTGAAGTGAAATTATCACAGTCACTAGACCCGAGCACATTATTTCATCTC
    ACTGACAATGAGGCAGGAGATCGCTTTTATATGCGTTTGAATGATAATCGAACGTCATAT
    TTTGGCTACAAAGCAATTCAATTATTCAAAAATAATTCTAAAAATAAACAATCTATTTTT
    AAAGACTGGGAAAAATTAAAACATAACATCACATTTATACATCCGCAATCTGAGAAACAT
    CATCTTCGAGTTGTTGGAGGGTTTCAATTTTCAAGTCATAAATCAGATGATGAATGGCGA
    GAGTTTGGACTAAATCATTTTGTATTACCTGAAGTTTTAATTTCAACTGATAATAATGGG
    ACATTTTTAACTTATACAGTTAAAAGGGAAAGTTTTACTGTTGAGGCATTGAACGATTTA
    ATGGATTTGTTCAACAATATATCGGATATAGATGTGGACGAGCAAATTGGGGAAATTACT
    AGAAATGAAGATATTTATAAAGATGACTGGCGTCAACTTGTAGTAGAAGCTATAGAATCT
    ATTAATAATGAAGAAAAAATTGTACTAGCACGTAGACGGTTAATAAAGTTCGATAAAGAT
    ATCAGTATTCCATATATTCTAAAGCAAGCATATTCTAAAGAAAAAAACAGTTATATATTC
    TTGTTAGAATCACAAGATTCTATTTTCTTTTCACAAACACCTGAACAATTAATAAAGGTC
    AATAATAAAATACTATCGACTAAAGCTGTAGCAGGTACAATTAAACGTTCACAAGATGAG
    GACGAAGATACAAAAAATGTTGAAGCATTTTTAAAAGATAATAAAAACTTAATCGAACAT
    CGATTTGTTGTTGACAGTATTTTACATGATATTAAACCTTATATCACTGAATTACATTAT
    GATAAGACGCCTAAAATTCTAAAAAATGATCATTTATATCACTTGTACACTGAAATAAAG
    GCGCCACTGAAGGATGATTCGTATATTAGTTTAATTGATCATTTACATCCAACACCTGCT
    TTAGGTGGCTATCCAAAAGAATTTGCGATGGATTTTATTGAACAGAAAGAATTTGGTACA
    CGAGGATTATATGGTGCGCCGGTTGGCTATATAGATATATATGATGATTGTGAATTTATT
    GTTGCAATTCGTTCGATGCTTATTAAGAAAGCACAAGCAACTTTATTTGCTGGGTGTGGC
    ATTGTTAAAGATTCTGATCCAGATAGTGAATTGGCAGAAACGAACCTTAAGTTCACACCT
    ATGATGAATGCATTAGGAGTCGATATGAATGGGAAATCATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1362

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SA0895 [new locus tag: SA_RS05080 ]
  • symbol: SA0895
  • description: hypothetical protein
  • length: 453
  • theoretical pI: 5.09672
  • theoretical MW: 52244.7
  • GRAVY: -0.459382

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersMenaquinone and ubiquinoneisochorismate synthase (TIGR00543; EC 5.4.4.2; HMM-score: 412)
    MetabolismAmino acid biosynthesisAromatic amino acid familyanthranilate synthase component I (TIGR01820; EC 4.1.3.27; HMM-score: 152.6)
    MetabolismAmino acid biosynthesisAromatic amino acid familyanthranilate synthase component I (TIGR00564; EC 4.1.3.27; HMM-score: 139.1)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersFolic acidaminodeoxychorismate synthase, component I (TIGR00553; EC 2.6.1.85; HMM-score: 118)
    aminodeoxychorismate synthase, component I (TIGR01824; EC 2.6.1.85; HMM-score: 106.5)
    salicylate synthase (TIGR03494; EC 4.1.3.-,5.4.4.2; HMM-score: 89.9)
    MetabolismAmino acid biosynthesisAromatic amino acid familyanthranilate synthase component I (TIGR00565; EC 4.1.3.27; HMM-score: 82.8)
    MetabolismAmino acid biosynthesisAromatic amino acid familyanthranilate synthase (TIGR01815; EC 4.1.3.27; HMM-score: 61.6)
    aminodeoxychorismate synthase (TIGR01823; EC 2.6.1.85; HMM-score: 60.4)
  • TheSEED:  
    Cofactors, Vitamins, Prosthetic Groups, PigmentsQuinone cofactorsMenaquinone and Phylloquinone Biosynthesis Menaquinone-specific isochorismate synthase (EC 5.4.4.2) 
    Quinone cofactorsMenaquinone biosynthesis from chorismate via 1,4-dihydroxy-2-naphthoate Menaquinone-specific isochorismate synthase (EC 5.4.4.2) 
  • PFAM:
    no clan definedChorismate_bind; chorismate binding enzyme (PF00425; HMM-score: 217.3)
    ATP_synthase (CL0255) vATP-synt_E; ATP synthase (E/31 kDa) subunit (PF01991; HMM-score: 16.1)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.117
    • Ymax_pos: 47
    • Cmax: 0.12
    • Cmax_pos: 40
    • Smax: 0.165
    • Smax_pos: 44
    • Smean: 0.089
    • D: 0.106
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MATGVLEDDIVKEIYGSSKEWVSVEVKLSQSLDPSTLFHLTDNEAGDRFYMRLNDNRTSYFGYKAIQLFKNNSKNKQSIFKDWEKLKHNITFIHPQSEKHHLRVVGGFQFSSHKSDDEWREFGLNHFVLPEVLISTDNNGTFLTYTVKRESFTVEALNDLMDLFNNISDIDVDEQIGEITRNEDIYKDDWRQLVVEAIESINNEEKIVLARRRLIKFDKDISIPYILKQAYSKEKNSYIFLLESQDSIFFSQTPEQLIKVNNKILSTKAVAGTIKRSQDEDEDTKNVEAFLKDNKNLIEHRFVVDSILHDIKPYITELHYDKTPKILKNDHLYHLYTEIKAPLKDDSYISLIDHLHPTPALGGYPKEFAMDFIEQKEFGTRGLYGAPVGYIDIYDDCEFIVAIRSMLIKKAQATLFAGCGIVKDSDPDSELAETNLKFTPMMNALGVDMNGKS

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]