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NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_1930 [new locus tag: SAUSA300_RS10590 ]
  • pan locus tag?: SAUPAN005048000
  • symbol: SAUSA300_1930
  • pan gene symbol?:
  • synonym:
  • product: phi77 ORF001-like protein, phage tail tape measure protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAUSA300_1930 [new locus tag: SAUSA300_RS10590 ]
  • symbol: SAUSA300_1930
  • product: phi77 ORF001-like protein, phage tail tape measure protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2096778..2101307
  • length: 4530
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGGAGAAAGAATAAAAGGTTTATCTATAGGTTTGGATTTAGATGCAGCAAATTTAAAT
    AGATCATTTGCAGAAATCAAACGAAACTTTAAAACTTTAAATTCTGACTTAAAATTAACA
    GGCAACAACTTCAAATATACCGAAAAATCAACTGATAGTTACAAACAAAGGATTAAAGAA
    CTTGATGGAACTATCACAGGTTATAAGAAAAACGTTGATGATTTAGCTAAGCAATATGAC
    AAGGTATCTCAAGAACAGGGCGAAAACAGTGCAGGAGCTCAAAAGTTACGACAAGAATAT
    AACAAACAAGCAAATGAACTGAATTATTTAGAAAGAGAATTACAAAAAACATCAGCCGAA
    TTTGAAGAGTTCAAAAAAGCTCAAGTTGAAGCTCAAAGAATGGCAGAAAGTGGCTGGGGA
    AAAACCAGTAAAGTTTTTGAAAGTATGGGACCTAAATTAACAAAAATGGGTGATGGTTTA
    AAATCCATTGGTAAAGGCATGATGATTGGTGTAACTGCACCTGTTTTAGGTATCGCAGCA
    GCATCAGGAAAAGCTTTTGCAGAAGTTGATAAAGGTTTAGATACTGTTACTCAAGCAACA
    GGCGCAACAGGCAGTGAATTAAAAAAATTGCAGAACTCATTTAAAGATGTTTATGGCAAT
    TTTCCAGCAGATGCTGAAACTGTTGGTGGAGTTTTAGGAGAAGTTAATACAAGATTAGGT
    TTTACAGGTAAAGAACTTGAAAATGCCACAGAGTCATTCTTGAAATTCAGTCATATAACA
    GGTTCTGACGGTGTGCAAGCCGTACAGTTAATTACCCGTGCAATGGGCGATGCAGGTATC
    GAAGCAAGTGAATATCAAAGTGTTTTGGATATGGTAGCAAAAGCGGCGCAAGCTAGTGGG
    ATAAGTGTTGACACATTAGCTGATAGTATTACTAAATACGGCGCTCCAATGAGAGCTATG
    GGCTTTGAGATGAAAGAATCAATTGCTTTATTCTCTCAATGGGAAAAGTCAGGCGTTAAT
    ACTGAAATAGCATTCAGTGGTTTGAAAAAAGCTATATCAAATTGGGGTAAAGCTGGTAAA
    AACCCAAGAGAAGAATTTAAGAAGACATTAGCAGAAATTGAAAAGACGCCGGATATAGCT
    AGCGCAACTATTTTAGCGATTGAAGCATTTGGTGCAAAGGCAGGTCCTGATTTAGCAGAC
    GCTATTAAAGGTGGTCGCTTTAGTTATCAAGAATTTTTAAAAACTATTGAAGATTCCCAA
    GGCACAGTAAACCAAACATTTAAAGATTCTGAAAGTGGCTCCGAAAGATTTAAAGTAGCA
    ATGAATAAATTAAAATTAGTAGGTGCTGATGTATGGGCTTCTATTGAAAGTGCGTTTGCT
    CCAGTCATGGAAGAATTAATAAAAAAGCTATCAGTCGCTGTCGATTGGTTTTCGAATTTA
    AGTGATGGTTCTAAAAGATCAATTGTTATTTTCGGTGGTATTGCTGCTGCAATTGGTCCT
    GTAGTTTTTGGGTTAGGTGCATTTATAAGCACAATTGGTAATGCAGTAACTGTATTAGCC
    CCACTATTAGCTGGTATTGCAAAGGCTGATGGATTAATTAGTTTTTTATCGACTAAAGTA
    CCTATATTAGGAACTGTCTTCACAGCTTTAACTGGTCCAATTGGCATTGTATTAGGTGTT
    TTAGCTGGCCTAGCAGTCGCATTTACAATTGCTTATAAGAAATCTGAAACATTTAGAAAT
    TTTGTTAATGATGCAATTGAAAGTGTTAAACAAACATTTAGTAATTTTATTCAATTTATT
    CAACCTTTCATTGATTCCGTTAAAAACGTCTTTAAACAAGCGGTTTCAGCAATCGTTGAT
    TTCGCTAAAGATATTTGGAGTCAAATTAATGGATTCTTTAATGAAAACGGAATTTCTATT
    GTTCAAGCGCTTCAAAATATATGCAATTTTATCAAAGTTATATTTGAATTTATCTTAAAT
    TTTGTAATTAAACCAATAATGTTTGCAATCTGGCAAGTGATGCAATTTATTTGGCCGGCG
    GTTAAAGCCTTGATTGTCAGTACTTGGGAGAATATAAAAGGTGTGATACAAGGAACTTTA
    AATATCATACTAGGTTTAATTAAGTTCTTCTCAAGTTTATTTACTGGAGATTGGCGAGGA
    GTTTGGGACGCGATTATTATGATTCTTAAAGGAGTCGTTCAATTAATATGGAATTTAATT
    CAATTATGGTTTGTAGGCAAAATACTTGGCGTTGTTAGGTACTTTGGCGGATTGCTAAAA
    GGATTAATAACAGGTATTTGGGACGTAATAAAAAGTATATTCAGTAAATCTTTATCAGCA
    ATTTGGAATGCGACAAAAAGTATTTTTGGATTCTTATTTAATAGTGTCAAATCAATTTTC
    ACGAATATGAAAAATTGGTTATCTAACACATGGAGTAGTATCCGTACGAATACGATAGGA
    AAAGCACAGTCATTATTTAGTGGTGTGAAATCAAAATTCACTAGTTTGTGGAATGCGACA
    AAAGATATTTTTAGTAATCTAAGAAATTGGATGGCAAATATTTGGAATTCCATTAAAGAT
    AATACGGTTGGAATTGCAAGTCGTTTATGGAGTAAAGTGCGTGGAATTTTTACAAATATG
    CGTGACGGCTTACAAAGTATTATCAGCAAAATTAAAAGTCATATCGGCGGTATGGTAGAT
    GCTATTAAAAAAGGACTTAATAAATTAATTGAAGGTTTAAACTGGGTCGGTGGTAAGCTG
    GGAATGGATAAAATACCTAAGTTACACACTGGTACAGAACACACACATACTACTACAAGA
    TTAGTTAAGAACGGTAAGATTGCGCGGGATACGTTCGCTACGGTTGGGGATAAAGGACGT
    GGAAATGGTCCGAATGGTTTCAGAAATGAAATAATTGAATTCCCTAATGGTAAACGTGTA
    ATCACACCTAATACAGATACTACCGCTTATTTACCTAAAGGCTCAAAAGTATACAACGGT
    ACACAAACTTATTCAATGTTAAACGGAACGCTTCCAAGATTTAGCTTAGGTACTATGTGG
    AAAGATATTAAATCCGGTGCATCATCGGCATTTAACTGGACAAAAGATCAAATAGGTAAA
    GGTACCAAATGGCTTGGCGATAAAGTTGGCGATGTTTTAGATTTTATGGAAAATCCTGGC
    AAACTTTTAAATTATATACTTGAAGCTTTTGGAATTGATTTCAATTCTTTAACTAAAGGT
    ATGGGAATTGCAGGCGACATAACAAAAGCTGCATGGTCTAAGATTAAGAAAAGTGCTACT
    GATTGGATAAAAGAAAATTTAGAAGCTATGGGCGGTGGCGATTTAGTCGGCGGAATATTA
    GACCCTGACAAAATTAATTATCATTATGGACGTACCGCAGCTTATACTGCTGCAACTGGA
    AGACCATTTCATGAAGGTGTCGATTTTCCATTTGTATATCAAGAAGTTAGAACGCCGATG
    GGTGGCAGACTTACAAGAATGCCATTTATGTCTGGTGGTTATGGTAATTATGTAAAAATT
    ACTAGTGGAGTTATCGATATGCTATTTGCGCATTTGAAAAACTTTAGCAAATCACCACCT
    AGTGGCACGATGGTAAAGCCCGGCGATGTTGTTGGTTTAACTGGTAATACCGGATTTAGT
    ACAGGACCACACTTACATTTTGAAATGAGAAGAAATGGACGTCACTTTGACCCTGAACCA
    TATTTAAGAAATGCAAAGAAAAAAGGTAGGTTATCAATTGGTGGCGGTGGTGCTACTTCT
    GGAAGTGGTGCAACTTATGCAAGCCGAGTAATCCGTCAAGCGCAAAGTATTTTAGGAGGA
    CGTTATAAAGGTAAGTGGATTCATGACCAGATGATGCGAGTTGCAAAGCGTGAAAGTAAC
    TATCAATCAAATGCAGTGAATAATTGGGATATTAATGCTCAAAGAGGAGACCCATCAAGA
    GGATTATTCCAAATCATCGGCTCAACTTTTAGAGCAAACGCTAAACGTGGATATACTAAC
    TTTAATAATCCGGTTCATCAAGGTATTTCAGCAATGCAGTACATTGTTAGACGCTACGGT
    TGGGATGGATTTAAGCGTGCTGGTGATTACGCATATGCTACAGGTGGAAAAGTTTTTGAT
    GGTTGGTATAACTTAGGTGAAGACGGTCATCCAGAATGGATTATTCCAACAGATCCAGCT
    CGTAGAAATGATGCAATGAAGATGTTGCATTATGCAGCAGCAGAAGTAAGAGGGAGAAAA
    GCGAGTAAAAATAAGCGTCCTAGTCAATTGTCTAGTGTAAATGGGTTTGATGACCCAAGC
    TTATTATTGAAAATGATTGAACAACAGCAACAACAAATAGCTTTATTACTGAAAATAGCG
    CAATCCAACGATGTGATTGCAGATAAAGATTATCAGCCGATTATTGACGAATACGCTTTT
    GATAAAAAGGTGAACGCGTCTATAGAAAAGCGAGAAAGGCAAGAATCAACAAAAGTAAAG
    TTTAGAAAAGGAGGAATTGCTATTCAATGA
    60
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    240
    300
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    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2280
    2340
    2400
    2460
    2520
    2580
    2640
    2700
    2760
    2820
    2880
    2940
    3000
    3060
    3120
    3180
    3240
    3300
    3360
    3420
    3480
    3540
    3600
    3660
    3720
    3780
    3840
    3900
    3960
    4020
    4080
    4140
    4200
    4260
    4320
    4380
    4440
    4500
    4530

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAUSA300_1930 [new locus tag: SAUSA300_RS10590 ]
  • symbol: SAUSA300_1930
  • description: phi77 ORF001-like protein, phage tail tape measure protein
  • length: 1509
  • theoretical pI: 10.2249
  • theoretical MW: 165607
  • GRAVY: -0.194433

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Genetic information processingMobile and extrachromosomal element functionsProphage functionsphage tail tape measure protein, TP901 family, core region (TIGR01760; HMM-score: 65)
    Cellular processesCellular processesCell divisionchromosome segregation protein SMC (TIGR02168; HMM-score: 25.5)
    Genetic information processingDNA metabolismChromosome-associated proteinschromosome segregation protein SMC (TIGR02168; HMM-score: 25.5)
    Cellular processesCellular processesCell divisionchromosome segregation protein SMC (TIGR02169; HMM-score: 22)
    Genetic information processingDNA metabolismChromosome-associated proteinschromosome segregation protein SMC (TIGR02169; HMM-score: 22)
    two transmembrane protein (TIGR04527; HMM-score: 17.8)
    WXG100 family type VII secretion target (TIGR03930; HMM-score: 13.5)
    Unknown functionEnzymes of unknown specificityquinoprotein relay system zinc metallohydrolase 2 (TIGR04559; EC 3.-.-.-; HMM-score: 13.5)
    Genetic information processingDNA metabolismDNA replication, recombination, and repairDnaD domain protein (TIGR01446; HMM-score: 13.2)
    Genetic information processingMobile and extrachromosomal element functionsProphage functionsDnaD domain protein (TIGR01446; HMM-score: 13.2)
    SH3 domain protein (TIGR04211; HMM-score: 13.1)
    Genetic information processingProtein synthesistRNA aminoacylationserine--tRNA ligase (TIGR00414; EC 6.1.1.11; HMM-score: 11.3)
    Genetic information processingProtein fateProtein folding and stabilizationprefoldin, alpha subunit (TIGR00293; HMM-score: 7.3)
    phasin (TIGR01985; HMM-score: 6.7)
  • TheSEED:  
    Phages, Prophages, Transposable elements, PlasmidsPhages, ProphagesPhage tail proteins 2 Phage tail length tape-measure protein 
  • PFAM:
    no clan definedPhageMin_Tail; Phage-related minor tail protein (PF10145; HMM-score: 67.6)
    Hybrid (CL0105) Peptidase_M23; Peptidase family M23 (PF01551; HMM-score: 61.9)
    Lysozyme (CL0037) SLT; Transglycosylase SLT domain (PF01464; HMM-score: 24.1)
    no clan definedDUF948; Bacterial protein of unknown function (DUF948) (PF06103; HMM-score: 19.6)
    Laminin_I; Laminin Domain I (PF06008; HMM-score: 18.3)
    TPR_MLP1_2; TPR/MLP1/MLP2-like protein (PF07926; HMM-score: 17.1)
    EzrA; Septation ring formation regulator, EzrA (PF06160; HMM-score: 16.4)
    YtxH; YtxH-like protein (PF12732; HMM-score: 14.7)
    BCLiA (CL0551) APG6; Autophagy protein Apg6 (PF04111; HMM-score: 14.4)
    no clan definedBaculo_PEP_C; Baculovirus polyhedron envelope protein, PEP, C terminus (PF04513; HMM-score: 14.4)
    DUF745; Protein of unknown function (DUF745) (PF05335; HMM-score: 13.8)
    FlaC_arch; Flagella accessory protein C (FlaC) (PF05377; HMM-score: 13.6)
    Sec34; Sec34-like family (PF04136; HMM-score: 13.5)
    FapA; Flagellar Assembly Protein A (PF03961; HMM-score: 13.4)
    GAS; Growth-arrest specific micro-tubule binding (PF13851; HMM-score: 13.1)
    IL32; Interleukin 32 (PF15225; HMM-score: 12.5)
    YebO; YebO-like protein (PF13974; HMM-score: 12.4)
    BLOC1_2; Biogenesis of lysosome-related organelles complex-1 subunit 2 (PF10046; HMM-score: 12.3)
    SNARE-fusion (CL0445) Syntaxin; Syntaxin (PF00804; HMM-score: 12)
    HTH (CL0123) Mnd1; Mnd1 family (PF03962; HMM-score: 11)
    no clan definedDUF16; Protein of unknown function DUF16 (PF01519; HMM-score: 10.8)
    MscS_porin; Mechanosensitive ion channel porin domain (PF12795; HMM-score: 10.8)
    Exonuc_VII_L; Exonuclease VII, large subunit (PF02601; HMM-score: 10.7)
    Reo_sigma (CL0326) Reo_sigmaC; Reovirus sigma C capsid protein (PF04582; HMM-score: 10.7)
    no clan definedTMP_2; Prophage tail length tape measure protein (PF06791; HMM-score: 10.7)
    Goodbye; fungal STAND N-terminal Goodbye domain (PF17109; HMM-score: 10.3)
    CENP-F_leu_zip; Leucine-rich repeats of kinetochore protein Cenp-F/LEK1 (PF10473; HMM-score: 9.8)
    Tho2; Transcription factor/nuclear export subunit protein 2 (PF11262; HMM-score: 9.4)
    tRNA_bind_arm (CL0298) Seryl_tRNA_N; Seryl-tRNA synthetase N-terminal domain (PF02403; HMM-score: 9.1)
    no clan definedCarbpep_Y_N; Carboxypeptidase Y pro-peptide (PF05388; HMM-score: 7.8)
    Fib_alpha; Fibrinogen alpha/beta chain family (PF08702; HMM-score: 7.5)
    HIP1_clath_bdg; Clathrin-binding domain of Huntingtin-interacting protein 1 (PF16515; HMM-score: 7.2)
    DASH_Spc34; DASH complex subunit Spc34 (PF08657; HMM-score: 7.1)
    DUF4407; Domain of unknown function (DUF4407) (PF14362; HMM-score: 7.1)
    HAUS-augmin3; HAUS augmin-like complex subunit 3 (PF14932; HMM-score: 6.2)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 2
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.135
    • Ymax_pos: 25
    • Cmax: 0.152
    • Cmax_pos: 25
    • Smax: 0.186
    • Smax_pos: 24
    • Smean: 0.107
    • D: 0.124
  • predicted transmembrane helices (TMHMM): 6

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MGERIKGLSIGLDLDAANLNRSFAEIKRNFKTLNSDLKLTGNNFKYTEKSTDSYKQRIKELDGTITGYKKNVDDLAKQYDKVSQEQGENSAGAQKLRQEYNKQANELNYLERELQKTSAEFEEFKKAQVEAQRMAESGWGKTSKVFESMGPKLTKMGDGLKSIGKGMMIGVTAPVLGIAAASGKAFAEVDKGLDTVTQATGATGSELKKLQNSFKDVYGNFPADAETVGGVLGEVNTRLGFTGKELENATESFLKFSHITGSDGVQAVQLITRAMGDAGIEASEYQSVLDMVAKAAQASGISVDTLADSITKYGAPMRAMGFEMKESIALFSQWEKSGVNTEIAFSGLKKAISNWGKAGKNPREEFKKTLAEIEKTPDIASATILAIEAFGAKAGPDLADAIKGGRFSYQEFLKTIEDSQGTVNQTFKDSESGSERFKVAMNKLKLVGADVWASIESAFAPVMEELIKKLSVAVDWFSNLSDGSKRSIVIFGGIAAAIGPVVFGLGAFISTIGNAVTVLAPLLAGIAKADGLISFLSTKVPILGTVFTALTGPIGIVLGVLAGLAVAFTIAYKKSETFRNFVNDAIESVKQTFSNFIQFIQPFIDSVKNVFKQAVSAIVDFAKDIWSQINGFFNENGISIVQALQNICNFIKVIFEFILNFVIKPIMFAIWQVMQFIWPAVKALIVSTWENIKGVIQGTLNIILGLIKFFSSLFTGDWRGVWDAIIMILKGVVQLIWNLIQLWFVGKILGVVRYFGGLLKGLITGIWDVIKSIFSKSLSAIWNATKSIFGFLFNSVKSIFTNMKNWLSNTWSSIRTNTIGKAQSLFSGVKSKFTSLWNATKDIFSNLRNWMANIWNSIKDNTVGIASRLWSKVRGIFTNMRDGLQSIISKIKSHIGGMVDAIKKGLNKLIEGLNWVGGKLGMDKIPKLHTGTEHTHTTTRLVKNGKIARDTFATVGDKGRGNGPNGFRNEIIEFPNGKRVITPNTDTTAYLPKGSKVYNGTQTYSMLNGTLPRFSLGTMWKDIKSGASSAFNWTKDQIGKGTKWLGDKVGDVLDFMENPGKLLNYILEAFGIDFNSLTKGMGIAGDITKAAWSKIKKSATDWIKENLEAMGGGDLVGGILDPDKINYHYGRTAAYTAATGRPFHEGVDFPFVYQEVRTPMGGRLTRMPFMSGGYGNYVKITSGVIDMLFAHLKNFSKSPPSGTMVKPGDVVGLTGNTGFSTGPHLHFEMRRNGRHFDPEPYLRNAKKKGRLSIGGGGATSGSGATYASRVIRQAQSILGGRYKGKWIHDQMMRVAKRESNYQSNAVNNWDINAQRGDPSRGLFQIIGSTFRANAKRGYTNFNNPVHQGISAMQYIVRRYGWDGFKRAGDYAYATGGKVFDGWYNLGEDGHPEWIIPTDPARRNDAMKMLHYAAAEVRGRKASKNKRPSQLSSVNGFDDPSLLLKMIEQQQQQIALLLKIAQSNDVIADKDYQPIIDEYAFDKKVNASIEKRERQESTKVKFRKGGIAIQ

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]