From AureoWiki
Jump to: navigation, search
NCBI: 10-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_2129 [new locus tag: SAUSA300_RS11725 ]
  • pan locus tag?: SAUPAN005559000
  • symbol: SAUSA300_2129
  • pan gene symbol?:
  • synonym:
  • product: putative hemolysin III

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAUSA300_2129 [new locus tag: SAUSA300_RS11725 ]
  • symbol: SAUSA300_2129
  • product: putative hemolysin III
  • replicon: chromosome
  • strand: -
  • coordinates: 2303099..2303782
  • length: 684
  • essential: unknown other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGTCCAAATCAACTAAAAAAAGTAATGACACAATCGTAGAAACATTTAAGGATATCATC
    CCTTTATCATTTGGAGAAGAAATTGGTAATGCGGCATCTCATGGTGTTGCAGCTTTGTTA
    ACATTATTAGTGTTACCCTATGCGGCTGTGCACAGTTATATTGCACATGGTACGCTTGCG
    TCTGTGAGTATGTCTATTTATGTTATTTCTATCTTTATGATGTTTATTTCATCTACTATT
    TATCATTCTATGCAAAATGAAACACCTCATAAATATATTTTAAGGATTATCGATCACAGT
    ATGATTTACGTAGCAATTTCTGGTACATATACGCCTATTTTGTTAACAGTTGTAGGTGGT
    TGGATAGGTTGGACAGTATTTATTTTATTATGGGGAACGACACTTTGGGGGATTTTATAT
    AAATCCATAGCTGTCAAAGTTAACCAAAAGTTAAGCTTAATTGTCTATTTAGTGATGGGC
    TGGGTAGGTATTATCTTGTTACCAATTATTTTTATTCGCACATCATGGATATTCATATTA
    TTCATTTTCTTAGGTGGTGTGTCTTATACAATTGGTGCATGGTTTTATGCACAAAAGAAT
    AGACCATATTTTCATATGATTTGGCATATTTTTATTGTAATAGCATCACTATTGCATTTA
    ATTGCAATCTTATATTTTATGTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    684

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAUSA300_2129 [new locus tag: SAUSA300_RS11725 ]
  • symbol: SAUSA300_2129
  • description: putative hemolysin III
  • length: 227
  • theoretical pI: 9.66153
  • theoretical MW: 25675.6
  • GRAVY: 0.929515

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    channel protein, hemolysin III family (TIGR01065; HMM-score: 176)
  • TheSEED:  
    Hemolysin III 
  • PFAM:
    GPCR_A (CL0192) HlyIII; Haemolysin-III related (PF03006; HMM-score: 104.9)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 7
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: -0.5
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.149
    • Ymax_pos: 55
    • Cmax: 0.166
    • Cmax_pos: 55
    • Smax: 0.268
    • Smax_pos: 47
    • Smean: 0.116
    • D: 0.136
  • predicted transmembrane helices (TMHMM): 7

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MSKSTKKSNDTIVETFKDIIPLSFGEEIGNAASHGVAALLTLLVLPYAAVHSYIAHGTLASVSMSIYVISIFMMFISSTIYHSMQNETPHKYILRIIDHSMIYVAISGTYTPILLTVVGGWIGWTVFILLWGTTLWGILYKSIAVKVNQKLSLIVYLVMGWVGIILLPIIFIRTSWIFILFIFLGGVSYTIGAWFYAQKNRPYFHMIWHIFIVIASLLHLIAILYFM

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulators: CcpA, LexA
    CcpA (TF) important in Carbon catabolism  RegPrecise    transcription unit transferred from N315 data RegPrecise
    LexA (TF) important in SOS response  RegPrecise    transcription unit transferred from N315 data RegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]